Genetic Diversity and Population Structure of Barley Cultivars Released in Turkey and Bulgaria using iPBS-retrotransposon and SCoT markers
To improve quantitative traits, it is essential to acknowledge genetic structure and diversity of the crop plants. In this study, 54 barley cultivars released from 1963 to date by different institutes in both Turkey and Bulgaria were screened with 18 iPBS and four SCoT markers to evaluate population...
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| Language: | English |
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Ankara University
2022-04-01
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| Series: | Journal of Agricultural Sciences |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1599703 |
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| author | Emre İlhan Ayşe Gül Kasapoğlu Ertuğrul Filiz Arash Hossein Pour Kamil Haliloğlu Ziya Dumlupınar Hüseyin Güngör Dragomir Valchev Darina Valcheva |
| author_facet | Emre İlhan Ayşe Gül Kasapoğlu Ertuğrul Filiz Arash Hossein Pour Kamil Haliloğlu Ziya Dumlupınar Hüseyin Güngör Dragomir Valchev Darina Valcheva |
| author_sort | Emre İlhan |
| collection | DOAJ |
| description | To improve quantitative traits, it is essential to acknowledge genetic structure and diversity of the crop plants. In this study, 54 barley cultivars released from 1963 to date by different institutes in both Turkey and Bulgaria were screened with 18 iPBS and four SCoT markers to evaluate population structure and genetic diversity. According to the results, while total polymorphic band numbers was identified as 560, the polymorphic ones were found as 530 (438 and 92 amplified bands for iPBS and SCoT markers, respectively). In addition, the average polymorphic band number was found as 24.09. While the average polymorphism information content (PIC) value was 0.48, the average PIC value was 0.48 for iPBS and 0.48 for SCoT markers. The highest PIC value was determined as 0.50. The highest effective number of alleles, Shannon’s information index, and Nei’s genetic diversity were detected from the iPBS2271 marker at 1.61, 0.52 and 0.35, respectively among the iPBS markers while the highest values were obtained from SCoT-71 marker as 1.55, 0.32 and 0.48, respectively. As a result of a distribution of the 530 amplified bands in 54 barley cultivars, structure analysis showed that the subpopulations in the barley cultivars as a value of k=5. The average expected heterozygosity and fixation indices were identified as 0.234 and 0.322, respectively. Based on DICE similarity index, Martı and Zahir cultivars were found the most similar barley cultivars with 75% genetic similarity, whereas Özdemir and Karatay 94 and Tosunpaşa and Konevi cultivars were found 73% similar. On the other hand, Bayrak and Avcı-2002 were found the most diverse cultivars with 19.9% genetic similarity. As a result, the barley cultivars released in Turkey and Bulgaria were found varying and, the genetic diversity and statistics index analysis indicated that iPBS and SCoT markers are powerful markers to perform genetic diversity analysis. |
| format | Article |
| id | doaj-art-250515f0c19e4374a64d259faed0bf00 |
| institution | OA Journals |
| issn | 1300-7580 2148-9297 |
| language | English |
| publishDate | 2022-04-01 |
| publisher | Ankara University |
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| series | Journal of Agricultural Sciences |
| spelling | doaj-art-250515f0c19e4374a64d259faed0bf002025-08-20T01:58:51ZengAnkara UniversityJournal of Agricultural Sciences1300-75802148-92972022-04-0128223925010.15832/ankutbd.88622145Genetic Diversity and Population Structure of Barley Cultivars Released in Turkey and Bulgaria using iPBS-retrotransposon and SCoT markersEmre İlhan0Ayşe Gül Kasapoğlu1Ertuğrul Filiz2Arash Hossein Pour3Kamil Haliloğlu4Ziya Dumlupınar5Hüseyin Güngör6Dragomir Valchev7Darina Valcheva8ERZURUM TEKNİK ÜNİVERSİTESİERZURUM TEKNİK ÜNİVERSİTESİDÜZCE ÜNİVERSİTESİATATÜRK ÜNİVERSİTESİATATÜRK ÜNİVERSİTESİKAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİDüzce Üniversitesi Ziraat Fakültesi Tarla Bitkileri BölümüInstitute of Agriculture , KarnobatInstitute of Agriculture , KarnobatTo improve quantitative traits, it is essential to acknowledge genetic structure and diversity of the crop plants. In this study, 54 barley cultivars released from 1963 to date by different institutes in both Turkey and Bulgaria were screened with 18 iPBS and four SCoT markers to evaluate population structure and genetic diversity. According to the results, while total polymorphic band numbers was identified as 560, the polymorphic ones were found as 530 (438 and 92 amplified bands for iPBS and SCoT markers, respectively). In addition, the average polymorphic band number was found as 24.09. While the average polymorphism information content (PIC) value was 0.48, the average PIC value was 0.48 for iPBS and 0.48 for SCoT markers. The highest PIC value was determined as 0.50. The highest effective number of alleles, Shannon’s information index, and Nei’s genetic diversity were detected from the iPBS2271 marker at 1.61, 0.52 and 0.35, respectively among the iPBS markers while the highest values were obtained from SCoT-71 marker as 1.55, 0.32 and 0.48, respectively. As a result of a distribution of the 530 amplified bands in 54 barley cultivars, structure analysis showed that the subpopulations in the barley cultivars as a value of k=5. The average expected heterozygosity and fixation indices were identified as 0.234 and 0.322, respectively. Based on DICE similarity index, Martı and Zahir cultivars were found the most similar barley cultivars with 75% genetic similarity, whereas Özdemir and Karatay 94 and Tosunpaşa and Konevi cultivars were found 73% similar. On the other hand, Bayrak and Avcı-2002 were found the most diverse cultivars with 19.9% genetic similarity. As a result, the barley cultivars released in Turkey and Bulgaria were found varying and, the genetic diversity and statistics index analysis indicated that iPBS and SCoT markers are powerful markers to perform genetic diversity analysis.https://dergipark.org.tr/tr/download/article-file/1599703barleygenetic diversityipbs-retrotransposonsscot markerstructure analysis |
| spellingShingle | Emre İlhan Ayşe Gül Kasapoğlu Ertuğrul Filiz Arash Hossein Pour Kamil Haliloğlu Ziya Dumlupınar Hüseyin Güngör Dragomir Valchev Darina Valcheva Genetic Diversity and Population Structure of Barley Cultivars Released in Turkey and Bulgaria using iPBS-retrotransposon and SCoT markers Journal of Agricultural Sciences barley genetic diversity ipbs-retrotransposons scot marker structure analysis |
| title | Genetic Diversity and Population Structure of Barley Cultivars Released in Turkey and Bulgaria using iPBS-retrotransposon and SCoT markers |
| title_full | Genetic Diversity and Population Structure of Barley Cultivars Released in Turkey and Bulgaria using iPBS-retrotransposon and SCoT markers |
| title_fullStr | Genetic Diversity and Population Structure of Barley Cultivars Released in Turkey and Bulgaria using iPBS-retrotransposon and SCoT markers |
| title_full_unstemmed | Genetic Diversity and Population Structure of Barley Cultivars Released in Turkey and Bulgaria using iPBS-retrotransposon and SCoT markers |
| title_short | Genetic Diversity and Population Structure of Barley Cultivars Released in Turkey and Bulgaria using iPBS-retrotransposon and SCoT markers |
| title_sort | genetic diversity and population structure of barley cultivars released in turkey and bulgaria using ipbs retrotransposon and scot markers |
| topic | barley genetic diversity ipbs-retrotransposons scot marker structure analysis |
| url | https://dergipark.org.tr/tr/download/article-file/1599703 |
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