Comparison of the Effectiveness of Two Different Pregnancy Detection Methods in Awassi Ewes

Aim of Study: This study aimed to compare several methods of pregnancy diagnosis in local Iraqi ewes including progesterone (P4) assay and the Ultrasongraphy (ULR) to identify the Sensitivty(Se), Specificity(Sp) and Accuracy(Acc) of the methods of diagnosis. Materials and Methods: This study was c...

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Main Authors: Ibrahim, Baraa Mahmood;, Al Rawi, Hani Muneb
Format: Article
Language:English
Published: College of Veterinary Medicine, University of Fallujah 2025-07-01
Series:مجلة الانبار للعلوم البيطرية
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Online Access:https://journals.lww.com/ajvs/_layouts/15/oaks.journals/downloadpdf.aspx?trckng_src_pg=ArticleViewer&an=02108118-202501000-00006
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author Ibrahim, Baraa Mahmood;
Al Rawi, Hani Muneb
author_facet Ibrahim, Baraa Mahmood;
Al Rawi, Hani Muneb
author_sort Ibrahim, Baraa Mahmood;
collection DOAJ
description Aim of Study: This study aimed to compare several methods of pregnancy diagnosis in local Iraqi ewes including progesterone (P4) assay and the Ultrasongraphy (ULR) to identify the Sensitivty(Se), Specificity(Sp) and Accuracy(Acc) of the methods of diagnosis. Materials and Methods: This study was carried Sixty of local Iraqi ewes with 3-5 years old. The experimental animals were subjected to an estrus synchronization. Blood samples were collected at 25 days of insemination, in order to measurement the level of P4 the first method. Pregnancy was diagnosed with Transrectal ULR (TRU) (second method) via TRU and transabdominal (TAU) –ULR, respectively. Results: The result showed that the Se, Sp, Acc of the P4 measurement at 25 days post-examination using the COBAS test were 95.1%, 42.1%, and 78.3% with (P < 0.05), respectively. The Se, Sp, and Acc at 30 days of examination by ULR were 85.4%, 78.9%, and 83.3% respectively, with (P < 0.05). The Se, Sp, and Acc at 60 days of examination by ultrasography were 100%, 100%, and 100% respectively, with (P < 0.05). The findings demonstrated that Se and Acc for P4 assay in day 25 were higher than ULR at day 30, but the Sp was lower than ULR method. Conclusion: It can be concluded that diagnosing pregnancy Depending on P4 assay at day 25 in sheep by measuring P4 concentration on day 25 after insemination and performing TR_ULR on day 30 and TA-ULR on day 60, are very effective methods for identifying pregnant sheep. Furthermore, the parameters for evaluating these methods (Se, Sp, Acc) improved as the pregnancy progressed, reaching their maximum on day 60 after insemination.
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publisher College of Veterinary Medicine, University of Fallujah
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spelling doaj-art-2501d9f9e27f45f2811cfc1f2fb70f942025-08-20T03:34:13ZengCollege of Veterinary Medicine, University of Fallujahمجلة الانبار للعلوم البيطرية1999-65272707-06032025-07-01181242710.4103/AJVS.AJVS_9_25Comparison of the Effectiveness of Two Different Pregnancy Detection Methods in Awassi EwesIbrahim, Baraa Mahmood; 0Al Rawi, Hani Muneb1Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Fallujah, Fallujah, IraqDepartment of Surgery and Obstetrics, College of Veterinary Medicine, University of Fallujah, Fallujah, IraqAim of Study: This study aimed to compare several methods of pregnancy diagnosis in local Iraqi ewes including progesterone (P4) assay and the Ultrasongraphy (ULR) to identify the Sensitivty(Se), Specificity(Sp) and Accuracy(Acc) of the methods of diagnosis. Materials and Methods: This study was carried Sixty of local Iraqi ewes with 3-5 years old. The experimental animals were subjected to an estrus synchronization. Blood samples were collected at 25 days of insemination, in order to measurement the level of P4 the first method. Pregnancy was diagnosed with Transrectal ULR (TRU) (second method) via TRU and transabdominal (TAU) –ULR, respectively. Results: The result showed that the Se, Sp, Acc of the P4 measurement at 25 days post-examination using the COBAS test were 95.1%, 42.1%, and 78.3% with (P < 0.05), respectively. The Se, Sp, and Acc at 30 days of examination by ULR were 85.4%, 78.9%, and 83.3% respectively, with (P < 0.05). The Se, Sp, and Acc at 60 days of examination by ultrasography were 100%, 100%, and 100% respectively, with (P < 0.05). The findings demonstrated that Se and Acc for P4 assay in day 25 were higher than ULR at day 30, but the Sp was lower than ULR method. Conclusion: It can be concluded that diagnosing pregnancy Depending on P4 assay at day 25 in sheep by measuring P4 concentration on day 25 after insemination and performing TR_ULR on day 30 and TA-ULR on day 60, are very effective methods for identifying pregnant sheep. Furthermore, the parameters for evaluating these methods (Se, Sp, Acc) improved as the pregnancy progressed, reaching their maximum on day 60 after insemination.https://journals.lww.com/ajvs/_layouts/15/oaks.journals/downloadpdf.aspx?trckng_src_pg=ArticleViewer&an=02108118-202501000-00006hormonespregnancy diagnosisprogesterone
spellingShingle Ibrahim, Baraa Mahmood;
Al Rawi, Hani Muneb
Comparison of the Effectiveness of Two Different Pregnancy Detection Methods in Awassi Ewes
مجلة الانبار للعلوم البيطرية
hormones
pregnancy diagnosis
progesterone
title Comparison of the Effectiveness of Two Different Pregnancy Detection Methods in Awassi Ewes
title_full Comparison of the Effectiveness of Two Different Pregnancy Detection Methods in Awassi Ewes
title_fullStr Comparison of the Effectiveness of Two Different Pregnancy Detection Methods in Awassi Ewes
title_full_unstemmed Comparison of the Effectiveness of Two Different Pregnancy Detection Methods in Awassi Ewes
title_short Comparison of the Effectiveness of Two Different Pregnancy Detection Methods in Awassi Ewes
title_sort comparison of the effectiveness of two different pregnancy detection methods in awassi ewes
topic hormones
pregnancy diagnosis
progesterone
url https://journals.lww.com/ajvs/_layouts/15/oaks.journals/downloadpdf.aspx?trckng_src_pg=ArticleViewer&an=02108118-202501000-00006
work_keys_str_mv AT ibrahimbaraamahmood comparisonoftheeffectivenessoftwodifferentpregnancydetectionmethodsinawassiewes
AT alrawihanimuneb comparisonoftheeffectivenessoftwodifferentpregnancydetectionmethodsinawassiewes