The effects of photosynthetic rate on respiration in light, starch/sucrose partitioning, and other metabolic fluxes within photosynthesis
Abstract In the future, plants may encounter increased light and elevated CO2 levels. How consequent alterations in photosynthetic rates will impact fluxes in photosynthetic carbon metabolism remains uncertain. Respiration in light (R L ) is pivotal in plant carbon balance and a key parameter in pho...
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Nature Portfolio
2025-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-88574-4 |
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| author | Yuan Xu Joshua A. M. Kaste Sean E. Weise Yair Shachar-Hill Thomas D. Sharkey |
| author_facet | Yuan Xu Joshua A. M. Kaste Sean E. Weise Yair Shachar-Hill Thomas D. Sharkey |
| author_sort | Yuan Xu |
| collection | DOAJ |
| description | Abstract In the future, plants may encounter increased light and elevated CO2 levels. How consequent alterations in photosynthetic rates will impact fluxes in photosynthetic carbon metabolism remains uncertain. Respiration in light (R L ) is pivotal in plant carbon balance and a key parameter in photosynthesis models. Understanding the dynamics of photosynthetic metabolism and R L under varying environmental conditions is essential for optimizing plant growth and agricultural productivity. However, measuring R L under high light and high CO2 (HLHC) conditions poses challenges using traditional gas exchange methods. In this study, we employed isotopically nonstationary metabolic flux analysis (INST-MFA) to estimate R L and investigate photosynthetic carbon flux, unveiling nuanced adjustments in Camelina sativa under HLHC. Despite numerous flux alterations in HLHC, R L remained stable. HLHC affects several factors influencing R L , such as starch and sucrose partitioning, v o /v c ratio, triose phosphate partitioning, and hexose kinase activity. Analysis of A/C i curve operational points reveals that HLHC’s major changes primarily stem from CO2 suppressing photorespiration. Integration of these fluxes into a simplified model predicts changes in CBC labeling under HLHC. This study extends our prior discovery that incomplete CBC labeling is due to unlabeled carbon reimported during R L , offering insights into manipulating labeling through adjustments in photosynthetic rates. |
| format | Article |
| id | doaj-art-24faac85aa034d21bc7413d2978b53ab |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-24faac85aa034d21bc7413d2978b53ab2025-08-20T02:56:06ZengNature PortfolioScientific Reports2045-23222025-03-0115111410.1038/s41598-025-88574-4The effects of photosynthetic rate on respiration in light, starch/sucrose partitioning, and other metabolic fluxes within photosynthesisYuan Xu0Joshua A. M. Kaste1Sean E. Weise2Yair Shachar-Hill3Thomas D. Sharkey4MSU-DOE Plant Research Laboratory, Michigan State UniversityDepartment of Biochemistry and Molecular Biology, Michigan State UniversityDepartment of Biochemistry and Molecular Biology, Michigan State UniversityDepartment of Plant Biology, Michigan State UniversityMSU-DOE Plant Research Laboratory, Michigan State UniversityAbstract In the future, plants may encounter increased light and elevated CO2 levels. How consequent alterations in photosynthetic rates will impact fluxes in photosynthetic carbon metabolism remains uncertain. Respiration in light (R L ) is pivotal in plant carbon balance and a key parameter in photosynthesis models. Understanding the dynamics of photosynthetic metabolism and R L under varying environmental conditions is essential for optimizing plant growth and agricultural productivity. However, measuring R L under high light and high CO2 (HLHC) conditions poses challenges using traditional gas exchange methods. In this study, we employed isotopically nonstationary metabolic flux analysis (INST-MFA) to estimate R L and investigate photosynthetic carbon flux, unveiling nuanced adjustments in Camelina sativa under HLHC. Despite numerous flux alterations in HLHC, R L remained stable. HLHC affects several factors influencing R L , such as starch and sucrose partitioning, v o /v c ratio, triose phosphate partitioning, and hexose kinase activity. Analysis of A/C i curve operational points reveals that HLHC’s major changes primarily stem from CO2 suppressing photorespiration. Integration of these fluxes into a simplified model predicts changes in CBC labeling under HLHC. This study extends our prior discovery that incomplete CBC labeling is due to unlabeled carbon reimported during R L , offering insights into manipulating labeling through adjustments in photosynthetic rates.https://doi.org/10.1038/s41598-025-88574-4Elevated CO2High lightMetabolic flux analysisPhotosynthesisPlant central metabolismStarch/sucrose partitioning |
| spellingShingle | Yuan Xu Joshua A. M. Kaste Sean E. Weise Yair Shachar-Hill Thomas D. Sharkey The effects of photosynthetic rate on respiration in light, starch/sucrose partitioning, and other metabolic fluxes within photosynthesis Scientific Reports Elevated CO2 High light Metabolic flux analysis Photosynthesis Plant central metabolism Starch/sucrose partitioning |
| title | The effects of photosynthetic rate on respiration in light, starch/sucrose partitioning, and other metabolic fluxes within photosynthesis |
| title_full | The effects of photosynthetic rate on respiration in light, starch/sucrose partitioning, and other metabolic fluxes within photosynthesis |
| title_fullStr | The effects of photosynthetic rate on respiration in light, starch/sucrose partitioning, and other metabolic fluxes within photosynthesis |
| title_full_unstemmed | The effects of photosynthetic rate on respiration in light, starch/sucrose partitioning, and other metabolic fluxes within photosynthesis |
| title_short | The effects of photosynthetic rate on respiration in light, starch/sucrose partitioning, and other metabolic fluxes within photosynthesis |
| title_sort | effects of photosynthetic rate on respiration in light starch sucrose partitioning and other metabolic fluxes within photosynthesis |
| topic | Elevated CO2 High light Metabolic flux analysis Photosynthesis Plant central metabolism Starch/sucrose partitioning |
| url | https://doi.org/10.1038/s41598-025-88574-4 |
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