Mechanism of miR-143 regulating ERK5 signaling pathway-mediated manganese-induced striatal neuronal apoptosis
Overexposure manganese can induce neurotoxicity and apoptosis, in addition, miRNAs are widely involved in the apoptotic process of neurons. Therefore, it is crucial to explore the mechanism of miRNAs in manganese-induced neuronal apoptosis and then to find potential targets. Our present study showed...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325006025 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849309570469462016 |
|---|---|
| author | Huan Mao Yidan Wei Cheng Liu Jianmin Yang Liyu Su Yuyan Cen Feng Wang Deyu Zhu Yu Deng Yan Li |
| author_facet | Huan Mao Yidan Wei Cheng Liu Jianmin Yang Liyu Su Yuyan Cen Feng Wang Deyu Zhu Yu Deng Yan Li |
| author_sort | Huan Mao |
| collection | DOAJ |
| description | Overexposure manganese can induce neurotoxicity and apoptosis, in addition, miRNAs are widely involved in the apoptotic process of neurons. Therefore, it is crucial to explore the mechanism of miRNAs in manganese-induced neuronal apoptosis and then to find potential targets. Our present study showed that miR-143 expression was increased in striatum after exposure high doses of manganese. Then, we upregulated miR-143 expression via stereotaxic, and overexpression of miR-143 promoted apoptosis in striatal neurons. Further studies showed a negative regulatory relationship between miR-143 and ERK5, miR-143 upregulation decreased ERK5 mRNA level, inhibiting ERK5 signaling pathway, increasing the expression of Bax and Caspase-3, and exacerbating the apoptosis of striatal neurons. In conclusion, these results suggest that overexpression of miR-143 inhibited the ERK5 signaling pathway and promoted manganese-induced apoptosis in striatal neurons. |
| format | Article |
| id | doaj-art-24e8ef5aec0c4c688856f00c8c6dbaa8 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-24e8ef5aec0c4c688856f00c8c6dbaa82025-08-20T03:54:07ZengElsevierEcotoxicology and Environmental Safety0147-65132025-06-0129811826610.1016/j.ecoenv.2025.118266Mechanism of miR-143 regulating ERK5 signaling pathway-mediated manganese-induced striatal neuronal apoptosisHuan Mao0Yidan Wei1Cheng Liu2Jianmin Yang3Liyu Su4Yuyan Cen5Feng Wang6Deyu Zhu7Yu Deng8Yan Li9Zunyi Medical University School of Public Health, Zunyi, Guizhou 563000, China; Chongqing Yuzhong District Center for Disease Control and Prevention, Chongqing 400010, ChinaZunyi Medical University School of Public Health, Zunyi, Guizhou 563000, ChinaZunyi Medical University School of Public Health, Zunyi, Guizhou 563000, ChinaZunyi Medical University School of Public Health, Zunyi, Guizhou 563000, ChinaZunyi Medical University School of Public Health, Zunyi, Guizhou 563000, ChinaZunyi Medical University School of Public Health, Zunyi, Guizhou 563000, ChinaZunyi Medical University School of Public Health, Zunyi, Guizhou 563000, ChinaZunyi Medical University School of Public Health, Zunyi, Guizhou 563000, ChinaDepartment of Environmental Health, School of Public Health, China Medical University, Shenyang, ChinaZunyi Medical University School of Public Health, Zunyi, Guizhou 563000, China; Key Laboratory of Maternal & Child Health and Exposure Science of Guizhou Higher Education Institutes, Zunyi, Guizhou 563000, China; Corresponding author at: School of Public Health, Zunyi Medical University, Zunyi, Guizhou 563000, China.Overexposure manganese can induce neurotoxicity and apoptosis, in addition, miRNAs are widely involved in the apoptotic process of neurons. Therefore, it is crucial to explore the mechanism of miRNAs in manganese-induced neuronal apoptosis and then to find potential targets. Our present study showed that miR-143 expression was increased in striatum after exposure high doses of manganese. Then, we upregulated miR-143 expression via stereotaxic, and overexpression of miR-143 promoted apoptosis in striatal neurons. Further studies showed a negative regulatory relationship between miR-143 and ERK5, miR-143 upregulation decreased ERK5 mRNA level, inhibiting ERK5 signaling pathway, increasing the expression of Bax and Caspase-3, and exacerbating the apoptosis of striatal neurons. In conclusion, these results suggest that overexpression of miR-143 inhibited the ERK5 signaling pathway and promoted manganese-induced apoptosis in striatal neurons.http://www.sciencedirect.com/science/article/pii/S0147651325006025ManganesemiR-143ERK5 signaling pathwayStriatalApoptosis |
| spellingShingle | Huan Mao Yidan Wei Cheng Liu Jianmin Yang Liyu Su Yuyan Cen Feng Wang Deyu Zhu Yu Deng Yan Li Mechanism of miR-143 regulating ERK5 signaling pathway-mediated manganese-induced striatal neuronal apoptosis Ecotoxicology and Environmental Safety Manganese miR-143 ERK5 signaling pathway Striatal Apoptosis |
| title | Mechanism of miR-143 regulating ERK5 signaling pathway-mediated manganese-induced striatal neuronal apoptosis |
| title_full | Mechanism of miR-143 regulating ERK5 signaling pathway-mediated manganese-induced striatal neuronal apoptosis |
| title_fullStr | Mechanism of miR-143 regulating ERK5 signaling pathway-mediated manganese-induced striatal neuronal apoptosis |
| title_full_unstemmed | Mechanism of miR-143 regulating ERK5 signaling pathway-mediated manganese-induced striatal neuronal apoptosis |
| title_short | Mechanism of miR-143 regulating ERK5 signaling pathway-mediated manganese-induced striatal neuronal apoptosis |
| title_sort | mechanism of mir 143 regulating erk5 signaling pathway mediated manganese induced striatal neuronal apoptosis |
| topic | Manganese miR-143 ERK5 signaling pathway Striatal Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325006025 |
| work_keys_str_mv | AT huanmao mechanismofmir143regulatingerk5signalingpathwaymediatedmanganeseinducedstriatalneuronalapoptosis AT yidanwei mechanismofmir143regulatingerk5signalingpathwaymediatedmanganeseinducedstriatalneuronalapoptosis AT chengliu mechanismofmir143regulatingerk5signalingpathwaymediatedmanganeseinducedstriatalneuronalapoptosis AT jianminyang mechanismofmir143regulatingerk5signalingpathwaymediatedmanganeseinducedstriatalneuronalapoptosis AT liyusu mechanismofmir143regulatingerk5signalingpathwaymediatedmanganeseinducedstriatalneuronalapoptosis AT yuyancen mechanismofmir143regulatingerk5signalingpathwaymediatedmanganeseinducedstriatalneuronalapoptosis AT fengwang mechanismofmir143regulatingerk5signalingpathwaymediatedmanganeseinducedstriatalneuronalapoptosis AT deyuzhu mechanismofmir143regulatingerk5signalingpathwaymediatedmanganeseinducedstriatalneuronalapoptosis AT yudeng mechanismofmir143regulatingerk5signalingpathwaymediatedmanganeseinducedstriatalneuronalapoptosis AT yanli mechanismofmir143regulatingerk5signalingpathwaymediatedmanganeseinducedstriatalneuronalapoptosis |