Characterization of Escherichia Coli Isolates from Patients with Urinary Tract Infections from Thabet Hospital-Tulkarm, Palestine
This study aimed to analyze phylogenetic groups, the presence of class I, II, III integrons and resistance phenotype in a collection of fifty E. coli strains isolated from urine specimens obtained from suspected cases with urinary tract infections at Thabet Hospital, During May-December 2012. The an...
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An-Najah National University
2016-03-01
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| Series: | مجلة جامعة النجاح للأبحاث العلوم الطبيعية |
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| Online Access: | https://journals.najah.edu/media/journals/full_texts/2_OG9s1nS.pdf |
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| author | Ghaleb Adwan Buthainah Issa |
| author_facet | Ghaleb Adwan Buthainah Issa |
| author_sort | Ghaleb Adwan |
| collection | DOAJ |
| description | This study aimed to analyze phylogenetic groups, the presence of class I, II, III integrons and resistance phenotype in a collection of fifty E. coli strains isolated from urine specimens obtained from suspected cases with urinary tract infections at Thabet Hospital, During May-December 2012. The antibiotic susceptibility testing for these isolates was done by disk diffusion method. The phylogenetic groups and class I, II and III integrons were determined by multiplex PCR. Statistical analysis was done by using Mann-Whitney U-Test (Two-tailed), Chi-square (χ2) test or Fisher exact test. The results showed that 36 (72%) of the studied strains, belonged to group D, 13 (26%) strains to group A, and 1 (2%) strain belonged to group B1. Twenty one (42%) of E. coli strains carried class I integrons. Prevalence of class I integrons in group D and group A was 44.4% and 30.8%, respectively. There was no significant difference in the mean of antibiotic resistance score for strains belonged to group D and carried class I integrons and those belonged to group A and harbored the same integron class. Antibiotic resistance has ranged from 24% for Gentamicin to 100% for Cefazolin and Erythromycin. There is a significant difference in the mean of antibiotic resistance scores for strains belonged to group D (8.4) and group A (6.5) (P = 6.2 x 10-4). Results showed that 2 large clusters depend on resistance/sensitive of strains to fluoroquinolones. Association of Trimethoprim/Sulphamethoxazole resistance with the D group showed statistically significant difference (P < 0.05) compared to group A. In conclusion, molecular analysis of 50 E. coli urine isolates exhibited a greater prevalence of class I integrons, a greater prevalence of phylogenetic group D, which possessed a higher resistance scores than group A. Urinary tract infections caused by such strains represent a clinical problem because of limited therapeutic options. |
| format | Article |
| id | doaj-art-24e4b7ce7b904bc99ea92305eb4a609b |
| institution | DOAJ |
| issn | 1727-2114 2311-8865 |
| language | English |
| publishDate | 2016-03-01 |
| publisher | An-Najah National University |
| record_format | Article |
| series | مجلة جامعة النجاح للأبحاث العلوم الطبيعية |
| spelling | doaj-art-24e4b7ce7b904bc99ea92305eb4a609b2025-08-20T02:56:27ZengAn-Najah National Universityمجلة جامعة النجاح للأبحاث العلوم الطبيعية1727-21142311-88652016-03-01301315210.35552/anujr.a.30.1.1171Characterization of Escherichia Coli Isolates from Patients with Urinary Tract Infections from Thabet Hospital-Tulkarm, PalestineGhaleb Adwan0Buthainah Issa1Department of Biology and Biotechnology, Faculty of Sciences, An-Najah National University, Nablus, PalestineNoneThis study aimed to analyze phylogenetic groups, the presence of class I, II, III integrons and resistance phenotype in a collection of fifty E. coli strains isolated from urine specimens obtained from suspected cases with urinary tract infections at Thabet Hospital, During May-December 2012. The antibiotic susceptibility testing for these isolates was done by disk diffusion method. The phylogenetic groups and class I, II and III integrons were determined by multiplex PCR. Statistical analysis was done by using Mann-Whitney U-Test (Two-tailed), Chi-square (χ2) test or Fisher exact test. The results showed that 36 (72%) of the studied strains, belonged to group D, 13 (26%) strains to group A, and 1 (2%) strain belonged to group B1. Twenty one (42%) of E. coli strains carried class I integrons. Prevalence of class I integrons in group D and group A was 44.4% and 30.8%, respectively. There was no significant difference in the mean of antibiotic resistance score for strains belonged to group D and carried class I integrons and those belonged to group A and harbored the same integron class. Antibiotic resistance has ranged from 24% for Gentamicin to 100% for Cefazolin and Erythromycin. There is a significant difference in the mean of antibiotic resistance scores for strains belonged to group D (8.4) and group A (6.5) (P = 6.2 x 10-4). Results showed that 2 large clusters depend on resistance/sensitive of strains to fluoroquinolones. Association of Trimethoprim/Sulphamethoxazole resistance with the D group showed statistically significant difference (P < 0.05) compared to group A. In conclusion, molecular analysis of 50 E. coli urine isolates exhibited a greater prevalence of class I integrons, a greater prevalence of phylogenetic group D, which possessed a higher resistance scores than group A. Urinary tract infections caused by such strains represent a clinical problem because of limited therapeutic options.https://journals.najah.edu/media/journals/full_texts/2_OG9s1nS.pdfantibiotic resistance scorese. coliphylogenetic groupsupecintegronspalestine. |
| spellingShingle | Ghaleb Adwan Buthainah Issa Characterization of Escherichia Coli Isolates from Patients with Urinary Tract Infections from Thabet Hospital-Tulkarm, Palestine مجلة جامعة النجاح للأبحاث العلوم الطبيعية antibiotic resistance scores e. coli phylogenetic groups upec integrons palestine. |
| title | Characterization of Escherichia Coli Isolates from Patients with Urinary Tract Infections from Thabet Hospital-Tulkarm, Palestine |
| title_full | Characterization of Escherichia Coli Isolates from Patients with Urinary Tract Infections from Thabet Hospital-Tulkarm, Palestine |
| title_fullStr | Characterization of Escherichia Coli Isolates from Patients with Urinary Tract Infections from Thabet Hospital-Tulkarm, Palestine |
| title_full_unstemmed | Characterization of Escherichia Coli Isolates from Patients with Urinary Tract Infections from Thabet Hospital-Tulkarm, Palestine |
| title_short | Characterization of Escherichia Coli Isolates from Patients with Urinary Tract Infections from Thabet Hospital-Tulkarm, Palestine |
| title_sort | characterization of escherichia coli isolates from patients with urinary tract infections from thabet hospital tulkarm palestine |
| topic | antibiotic resistance scores e. coli phylogenetic groups upec integrons palestine. |
| url | https://journals.najah.edu/media/journals/full_texts/2_OG9s1nS.pdf |
| work_keys_str_mv | AT ghalebadwan characterizationofescherichiacoliisolatesfrompatientswithurinarytractinfectionsfromthabethospitaltulkarmpalestine AT buthainahissa characterizationofescherichiacoliisolatesfrompatientswithurinarytractinfectionsfromthabethospitaltulkarmpalestine |