Effect of Different Partial Pressures on H<sub>2</sub> Production with <i>Parageobacillus thermoglucosidasius</i> DSM 6285
The ability of <i>Parageobacillus thermoglucosidasius</i> to produce H<sub>2</sub> from CO via the water–gas shift (WGS) reaction makes it a compelling microorganism for biofuels research. Optimizing this process requires evaluating parameters such as pressure. This study aim...
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MDPI AG
2024-11-01
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| Series: | Fermentation |
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| author | Magda Stephania Ardila Habibu Aliyu Pieter de Maayer Anke Neumann |
| author_facet | Magda Stephania Ardila Habibu Aliyu Pieter de Maayer Anke Neumann |
| author_sort | Magda Stephania Ardila |
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| description | The ability of <i>Parageobacillus thermoglucosidasius</i> to produce H<sub>2</sub> from CO via the water–gas shift (WGS) reaction makes it a compelling microorganism for biofuels research. Optimizing this process requires evaluating parameters such as pressure. This study aimed to understand how H<sub>2</sub> production is affected by increasing CO, N<sub>2</sub>, and H<sub>2</sub> partial pressures to 1.0, 2.0, and 3.0 bar. Increasing CO partial pressure can improve the solubility of the gas in the liquid phase. However, raising CO partial pressure to 3.0 bar had an inhibitory effect, delaying and reducing H<sub>2</sub> production. By contrast, increasing N<sub>2</sub> and H<sub>2</sub> partial pressures to 3.0 bar had positive effects, reaching a H<sub>2</sub> production of 9.2 mmol and 130 mmol, respectively. Analysis of the electron balance at the end of the fermentation process showed that the selectivity toward H<sub>2</sub> production reached 95%, with the remainder of electrons deriving from CO and glucose directed at organic acid production, mainly acetate, followed by formate. |
| format | Article |
| id | doaj-art-24e45b866eec4ff1b35aec86bd3c2351 |
| institution | OA Journals |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Fermentation |
| spelling | doaj-art-24e45b866eec4ff1b35aec86bd3c23512025-08-20T02:28:10ZengMDPI AGFermentation2311-56372024-11-01101159210.3390/fermentation10110592Effect of Different Partial Pressures on H<sub>2</sub> Production with <i>Parageobacillus thermoglucosidasius</i> DSM 6285Magda Stephania Ardila0Habibu Aliyu1Pieter de Maayer2Anke Neumann3Institute of Process Engineering in Life Science, Section II: Electrobiotechnology, Karlsruhe Institute of Technology, 76131 Karlsruhe, GermanyInstitute for Biological Interfaces, Section V: Biotechnology and Microbial Genetics, Karlsruhe Institute of Technology, 76131 Karlsruhe, GermanySchool of Molecular and Cell Biology, Faculty of Science, University of the Witwatersrand, Johannesburg 2050, South AfricaInstitute of Process Engineering in Life Science, Section II: Electrobiotechnology, Karlsruhe Institute of Technology, 76131 Karlsruhe, GermanyThe ability of <i>Parageobacillus thermoglucosidasius</i> to produce H<sub>2</sub> from CO via the water–gas shift (WGS) reaction makes it a compelling microorganism for biofuels research. Optimizing this process requires evaluating parameters such as pressure. This study aimed to understand how H<sub>2</sub> production is affected by increasing CO, N<sub>2</sub>, and H<sub>2</sub> partial pressures to 1.0, 2.0, and 3.0 bar. Increasing CO partial pressure can improve the solubility of the gas in the liquid phase. However, raising CO partial pressure to 3.0 bar had an inhibitory effect, delaying and reducing H<sub>2</sub> production. By contrast, increasing N<sub>2</sub> and H<sub>2</sub> partial pressures to 3.0 bar had positive effects, reaching a H<sub>2</sub> production of 9.2 mmol and 130 mmol, respectively. Analysis of the electron balance at the end of the fermentation process showed that the selectivity toward H<sub>2</sub> production reached 95%, with the remainder of electrons deriving from CO and glucose directed at organic acid production, mainly acetate, followed by formate.https://www.mdpi.com/2311-5637/10/11/592<i>Parageobacillus thermoglucosidasius</i>hydrogenwater–gas shift reactionCO-dehydrogenasepartial pressure |
| spellingShingle | Magda Stephania Ardila Habibu Aliyu Pieter de Maayer Anke Neumann Effect of Different Partial Pressures on H<sub>2</sub> Production with <i>Parageobacillus thermoglucosidasius</i> DSM 6285 Fermentation <i>Parageobacillus thermoglucosidasius</i> hydrogen water–gas shift reaction CO-dehydrogenase partial pressure |
| title | Effect of Different Partial Pressures on H<sub>2</sub> Production with <i>Parageobacillus thermoglucosidasius</i> DSM 6285 |
| title_full | Effect of Different Partial Pressures on H<sub>2</sub> Production with <i>Parageobacillus thermoglucosidasius</i> DSM 6285 |
| title_fullStr | Effect of Different Partial Pressures on H<sub>2</sub> Production with <i>Parageobacillus thermoglucosidasius</i> DSM 6285 |
| title_full_unstemmed | Effect of Different Partial Pressures on H<sub>2</sub> Production with <i>Parageobacillus thermoglucosidasius</i> DSM 6285 |
| title_short | Effect of Different Partial Pressures on H<sub>2</sub> Production with <i>Parageobacillus thermoglucosidasius</i> DSM 6285 |
| title_sort | effect of different partial pressures on h sub 2 sub production with i parageobacillus thermoglucosidasius i dsm 6285 |
| topic | <i>Parageobacillus thermoglucosidasius</i> hydrogen water–gas shift reaction CO-dehydrogenase partial pressure |
| url | https://www.mdpi.com/2311-5637/10/11/592 |
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