Enhancing Soil Phosphorus and Potassium Availability in Tea Plantation: The Role of Biochar, PGPR, and Phosphorus- and Potassium-Bearing Minerals

The co-application of biochar, plant growth-promoting rhizobacteria (PGPR), and phosphorus- and potassium-bearing minerals has emerged as a promising strategy for improving soil nutrient availability. However, the synergistic effects and impact factors that facilitate this optimization are yet to be...

Full description

Saved in:
Bibliographic Details
Main Authors: Wen Wei, Kunyu Li, Changjun Li, Siyu Wang, Lulu Li, Jinchuan Xie, Ting Li, Zijun Zhou, Shirong Zhang, Yulin Pu, Yongxia Jia, Xiaojing Liu, Xiaoxun Xu, Guiyin Wang
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Agronomy
Subjects:
Online Access:https://www.mdpi.com/2073-4395/15/6/1287
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849435445777137664
author Wen Wei
Kunyu Li
Changjun Li
Siyu Wang
Lulu Li
Jinchuan Xie
Ting Li
Zijun Zhou
Shirong Zhang
Yulin Pu
Yongxia Jia
Xiaojing Liu
Xiaoxun Xu
Guiyin Wang
author_facet Wen Wei
Kunyu Li
Changjun Li
Siyu Wang
Lulu Li
Jinchuan Xie
Ting Li
Zijun Zhou
Shirong Zhang
Yulin Pu
Yongxia Jia
Xiaojing Liu
Xiaoxun Xu
Guiyin Wang
author_sort Wen Wei
collection DOAJ
description The co-application of biochar, plant growth-promoting rhizobacteria (PGPR), and phosphorus- and potassium-bearing minerals has emerged as a promising strategy for improving soil nutrient availability. However, the synergistic effects and impact factors that facilitate this optimization are yet to be fully elucidated. To address this knowledge gap, we conducted a pot experiment to evaluate the effects of these amendments on tea yield and phosphorus (P)/potassium (K) availability, while employing Random Forest (RF) and Partial Least Squares Structural Equation Modeling (PLS-SEM) to reveal the underlying mechanisms driving these improvements. The results demonstrated that the tripartite combination significantly enhanced tea yield, leaf P/K concentrations, and soil available P (AP)/available K (AK) levels compared to individual applications or pairwise combinations. Analytical modeling identified <i>Chloroflexi</i> bacteria containing <i>pqqc</i> functional genes as key drivers of AP enhancement. The AP was further modulated by β-glucosidase activity, NaHCO<sub>3</sub>-P, and AK levels. Critical determinants of AK dynamics included phosphorus-solubilizing bacterial populations, catalase activity, and fundamental soil chemical properties. In summary, our research conclusively shows that the co-application of phosphorus- and potassium-bearing minerals, PGPR, and biochar represents an effective approach to enhancing P and K accessibility in soil, thereby offering a viable alternative to conventional P and K fertilizers in tea cultivation.
format Article
id doaj-art-24e192b3b9404452b63bed45461995e9
institution Kabale University
issn 2073-4395
language English
publishDate 2025-05-01
publisher MDPI AG
record_format Article
series Agronomy
spelling doaj-art-24e192b3b9404452b63bed45461995e92025-08-20T03:26:16ZengMDPI AGAgronomy2073-43952025-05-01156128710.3390/agronomy15061287Enhancing Soil Phosphorus and Potassium Availability in Tea Plantation: The Role of Biochar, PGPR, and Phosphorus- and Potassium-Bearing MineralsWen Wei0Kunyu Li1Changjun Li2Siyu Wang3Lulu Li4Jinchuan Xie5Ting Li6Zijun Zhou7Shirong Zhang8Yulin Pu9Yongxia Jia10Xiaojing Liu11Xiaoxun Xu12Guiyin Wang13College of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaSoil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaCollege of Environmental Science, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Environmental Science, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Environmental Science, Sichuan Agricultural University, Chengdu 611130, ChinaThe co-application of biochar, plant growth-promoting rhizobacteria (PGPR), and phosphorus- and potassium-bearing minerals has emerged as a promising strategy for improving soil nutrient availability. However, the synergistic effects and impact factors that facilitate this optimization are yet to be fully elucidated. To address this knowledge gap, we conducted a pot experiment to evaluate the effects of these amendments on tea yield and phosphorus (P)/potassium (K) availability, while employing Random Forest (RF) and Partial Least Squares Structural Equation Modeling (PLS-SEM) to reveal the underlying mechanisms driving these improvements. The results demonstrated that the tripartite combination significantly enhanced tea yield, leaf P/K concentrations, and soil available P (AP)/available K (AK) levels compared to individual applications or pairwise combinations. Analytical modeling identified <i>Chloroflexi</i> bacteria containing <i>pqqc</i> functional genes as key drivers of AP enhancement. The AP was further modulated by β-glucosidase activity, NaHCO<sub>3</sub>-P, and AK levels. Critical determinants of AK dynamics included phosphorus-solubilizing bacterial populations, catalase activity, and fundamental soil chemical properties. In summary, our research conclusively shows that the co-application of phosphorus- and potassium-bearing minerals, PGPR, and biochar represents an effective approach to enhancing P and K accessibility in soil, thereby offering a viable alternative to conventional P and K fertilizers in tea cultivation.https://www.mdpi.com/2073-4395/15/6/1287nutrient availabilitytea yieldrandom forestpartial least squares structural equation modelinginfluencing factors
spellingShingle Wen Wei
Kunyu Li
Changjun Li
Siyu Wang
Lulu Li
Jinchuan Xie
Ting Li
Zijun Zhou
Shirong Zhang
Yulin Pu
Yongxia Jia
Xiaojing Liu
Xiaoxun Xu
Guiyin Wang
Enhancing Soil Phosphorus and Potassium Availability in Tea Plantation: The Role of Biochar, PGPR, and Phosphorus- and Potassium-Bearing Minerals
Agronomy
nutrient availability
tea yield
random forest
partial least squares structural equation modeling
influencing factors
title Enhancing Soil Phosphorus and Potassium Availability in Tea Plantation: The Role of Biochar, PGPR, and Phosphorus- and Potassium-Bearing Minerals
title_full Enhancing Soil Phosphorus and Potassium Availability in Tea Plantation: The Role of Biochar, PGPR, and Phosphorus- and Potassium-Bearing Minerals
title_fullStr Enhancing Soil Phosphorus and Potassium Availability in Tea Plantation: The Role of Biochar, PGPR, and Phosphorus- and Potassium-Bearing Minerals
title_full_unstemmed Enhancing Soil Phosphorus and Potassium Availability in Tea Plantation: The Role of Biochar, PGPR, and Phosphorus- and Potassium-Bearing Minerals
title_short Enhancing Soil Phosphorus and Potassium Availability in Tea Plantation: The Role of Biochar, PGPR, and Phosphorus- and Potassium-Bearing Minerals
title_sort enhancing soil phosphorus and potassium availability in tea plantation the role of biochar pgpr and phosphorus and potassium bearing minerals
topic nutrient availability
tea yield
random forest
partial least squares structural equation modeling
influencing factors
url https://www.mdpi.com/2073-4395/15/6/1287
work_keys_str_mv AT wenwei enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals
AT kunyuli enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals
AT changjunli enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals
AT siyuwang enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals
AT lululi enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals
AT jinchuanxie enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals
AT tingli enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals
AT zijunzhou enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals
AT shirongzhang enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals
AT yulinpu enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals
AT yongxiajia enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals
AT xiaojingliu enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals
AT xiaoxunxu enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals
AT guiyinwang enhancingsoilphosphorusandpotassiumavailabilityinteaplantationtheroleofbiocharpgprandphosphorusandpotassiumbearingminerals