Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (<i>Hermetia illucens</i>) Larvae: A Comparison with Mealworm (<i>Tenebrio molitor</i>) Larvae
Insect larvae production offers the potential for large-scale synthesis of high-quality protein that can be used as feed or food. However, currently, there are limitations on the source of substrates for the insect larvae to use. One concern is the potential survival of animal pathogens within insec...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/13/12/1038 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850036134948634624 |
|---|---|
| author | Antoine Lecocq Anna Luiza Farias Alencar Christina M. Lazov Sheikh M. Rajiuddin Anette Bøtner Graham J. Belsham |
| author_facet | Antoine Lecocq Anna Luiza Farias Alencar Christina M. Lazov Sheikh M. Rajiuddin Anette Bøtner Graham J. Belsham |
| author_sort | Antoine Lecocq |
| collection | DOAJ |
| description | Insect larvae production offers the potential for large-scale synthesis of high-quality protein that can be used as feed or food. However, currently, there are limitations on the source of substrates for the insect larvae to use. One concern is the potential survival of animal pathogens within insect larvae if their feed is contaminated. In this study, the survival of a very stable virus, porcine parvovirus (PPV), within mealworm (<i>Tenebrio molitor</i>) and black soldier fly (BSF) (<i>Hermetia illucens</i>) larvae has been analyzed after oral ingestion of the virus. PPV genomic DNA could be readily detected by PCR in both species of larvae up until 9 days post ingestion (DPI), the end of the study period. Furthermore, infection of susceptible PK15 cells by PPV from homogenized mealworm larvae could be detected until at least 3 DPI, using an immunoperoxidase staining method and, up until 9 DPI, with a more sensitive real time PCR assay. Thus, PPV can remain infectious within mealworm larvae during their main growth phase through to their harvesting. However, it may be considered that PPV is exceptional in this respect since it displays unusual stability, e.g., to heat. |
| format | Article |
| id | doaj-art-24cb767f2e024e6b8041813301a51304 |
| institution | DOAJ |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-24cb767f2e024e6b8041813301a513042025-08-20T02:57:17ZengMDPI AGPathogens2076-08172024-11-011312103810.3390/pathogens13121038Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (<i>Hermetia illucens</i>) Larvae: A Comparison with Mealworm (<i>Tenebrio molitor</i>) LarvaeAntoine Lecocq0Anna Luiza Farias Alencar1Christina M. Lazov2Sheikh M. Rajiuddin3Anette Bøtner4Graham J. Belsham5Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, DenmarkDepartment of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, DenmarkDepartment of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, DenmarkDepartment of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, DenmarkDepartment of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, DenmarkDepartment of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, DenmarkInsect larvae production offers the potential for large-scale synthesis of high-quality protein that can be used as feed or food. However, currently, there are limitations on the source of substrates for the insect larvae to use. One concern is the potential survival of animal pathogens within insect larvae if their feed is contaminated. In this study, the survival of a very stable virus, porcine parvovirus (PPV), within mealworm (<i>Tenebrio molitor</i>) and black soldier fly (BSF) (<i>Hermetia illucens</i>) larvae has been analyzed after oral ingestion of the virus. PPV genomic DNA could be readily detected by PCR in both species of larvae up until 9 days post ingestion (DPI), the end of the study period. Furthermore, infection of susceptible PK15 cells by PPV from homogenized mealworm larvae could be detected until at least 3 DPI, using an immunoperoxidase staining method and, up until 9 DPI, with a more sensitive real time PCR assay. Thus, PPV can remain infectious within mealworm larvae during their main growth phase through to their harvesting. However, it may be considered that PPV is exceptional in this respect since it displays unusual stability, e.g., to heat.https://www.mdpi.com/2076-0817/13/12/1038insect larvaevirus survivalvirus ingestionvirus infectivity assays |
| spellingShingle | Antoine Lecocq Anna Luiza Farias Alencar Christina M. Lazov Sheikh M. Rajiuddin Anette Bøtner Graham J. Belsham Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (<i>Hermetia illucens</i>) Larvae: A Comparison with Mealworm (<i>Tenebrio molitor</i>) Larvae Pathogens insect larvae virus survival virus ingestion virus infectivity assays |
| title | Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (<i>Hermetia illucens</i>) Larvae: A Comparison with Mealworm (<i>Tenebrio molitor</i>) Larvae |
| title_full | Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (<i>Hermetia illucens</i>) Larvae: A Comparison with Mealworm (<i>Tenebrio molitor</i>) Larvae |
| title_fullStr | Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (<i>Hermetia illucens</i>) Larvae: A Comparison with Mealworm (<i>Tenebrio molitor</i>) Larvae |
| title_full_unstemmed | Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (<i>Hermetia illucens</i>) Larvae: A Comparison with Mealworm (<i>Tenebrio molitor</i>) Larvae |
| title_short | Use of a Novel Feeding System to Assess the Survival of a Very Stable Mammalian Virus, Porcine Parvovirus, Within Black Soldier Fly (<i>Hermetia illucens</i>) Larvae: A Comparison with Mealworm (<i>Tenebrio molitor</i>) Larvae |
| title_sort | use of a novel feeding system to assess the survival of a very stable mammalian virus porcine parvovirus within black soldier fly i hermetia illucens i larvae a comparison with mealworm i tenebrio molitor i larvae |
| topic | insect larvae virus survival virus ingestion virus infectivity assays |
| url | https://www.mdpi.com/2076-0817/13/12/1038 |
| work_keys_str_mv | AT antoinelecocq useofanovelfeedingsystemtoassessthesurvivalofaverystablemammalianvirusporcineparvoviruswithinblacksoldierflyihermetiaillucensilarvaeacomparisonwithmealwormitenebriomolitorilarvae AT annaluizafariasalencar useofanovelfeedingsystemtoassessthesurvivalofaverystablemammalianvirusporcineparvoviruswithinblacksoldierflyihermetiaillucensilarvaeacomparisonwithmealwormitenebriomolitorilarvae AT christinamlazov useofanovelfeedingsystemtoassessthesurvivalofaverystablemammalianvirusporcineparvoviruswithinblacksoldierflyihermetiaillucensilarvaeacomparisonwithmealwormitenebriomolitorilarvae AT sheikhmrajiuddin useofanovelfeedingsystemtoassessthesurvivalofaverystablemammalianvirusporcineparvoviruswithinblacksoldierflyihermetiaillucensilarvaeacomparisonwithmealwormitenebriomolitorilarvae AT anettebøtner useofanovelfeedingsystemtoassessthesurvivalofaverystablemammalianvirusporcineparvoviruswithinblacksoldierflyihermetiaillucensilarvaeacomparisonwithmealwormitenebriomolitorilarvae AT grahamjbelsham useofanovelfeedingsystemtoassessthesurvivalofaverystablemammalianvirusporcineparvoviruswithinblacksoldierflyihermetiaillucensilarvaeacomparisonwithmealwormitenebriomolitorilarvae |