Genomic analysis of Listeria monocytogenes strains from dairy products in Ethiopia
This study explored virulence genes, antibiotic resistance genes, and mobile genetic elements in 14 Listeria monocytogenes strains from milk and dairy products collected from different regions of Ethiopia. The strains were classified into two Multilocus Sequence Typing sequence types (ST2 and ST45)...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-04-01
|
| Series: | Frontiers in Bioinformatics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbinf.2025.1572241/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849725883568357376 |
|---|---|
| author | Mebrie Zemene Kinde Bizuayehu Kerisew Tegegne Eshetu Abebe Tesfaye Gessese |
| author_facet | Mebrie Zemene Kinde Bizuayehu Kerisew Tegegne Eshetu Abebe Tesfaye Gessese |
| author_sort | Mebrie Zemene Kinde |
| collection | DOAJ |
| description | This study explored virulence genes, antibiotic resistance genes, and mobile genetic elements in 14 Listeria monocytogenes strains from milk and dairy products collected from different regions of Ethiopia. The strains were classified into two Multilocus Sequence Typing sequence types (ST2 and ST45) and further grouped into clonal complexes (CC2) and different cgMLST types. Twenty-nine virulence genes were identified across all 14 strains, with lplA1 detected at higher levels in all strains except SAMN28661660. All L. monocytogenes strains also carried four antibiotic resistance genes (fosX, lin, norB, mprF), contributing to their ability to withstand multiple antimicrobial agents. Notably, no plasmids or mobile genetic elements were detected. Stress resistance genes, including stress survival islet 1 (SSI1_lmo0447), lmo 1800, and lmo1799, were identified in all strains. However, genes encoding for disinfectant resistance were not identified from all strains. LGI-2 was found in all the strains and none of the studied strains harbored LGI-1 and LGI-3. Conserved CRISPR-Cas systems were found in some strains. KEGG pathway analysis revealed that inlA and inlB genes facilitate bacterial internalization through host actin polymerization. Overall, the study provided crucial insights into the genomic features of L. monocytogenes in the Ethiopian dairy chain. It is crucial to establish continuous monitoring of L. monocytogenes in dairy products, improve sanitation, enforce stricter antibiotic usage and food safety regulations, and raise public awareness of associated risks. |
| format | Article |
| id | doaj-art-24c4b3ca81d8410fb8faee19ca434b47 |
| institution | DOAJ |
| issn | 2673-7647 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioinformatics |
| spelling | doaj-art-24c4b3ca81d8410fb8faee19ca434b472025-08-20T03:10:21ZengFrontiers Media S.A.Frontiers in Bioinformatics2673-76472025-04-01510.3389/fbinf.2025.15722411572241Genomic analysis of Listeria monocytogenes strains from dairy products in EthiopiaMebrie Zemene Kinde0Bizuayehu Kerisew1Tegegne Eshetu2Abebe Tesfaye Gessese3Department of Veterinary Biomedical Sciences, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, EthiopiaDepartment of Biology, Bahir Dar University, Bahir Dar, EthiopiaDepartment of Biomedical and Laboratory Science, College of Medicine and Health Science, University of Gondar, Gondar, EthiopiaDepartment of Veterinary Biomedical Sciences, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, EthiopiaThis study explored virulence genes, antibiotic resistance genes, and mobile genetic elements in 14 Listeria monocytogenes strains from milk and dairy products collected from different regions of Ethiopia. The strains were classified into two Multilocus Sequence Typing sequence types (ST2 and ST45) and further grouped into clonal complexes (CC2) and different cgMLST types. Twenty-nine virulence genes were identified across all 14 strains, with lplA1 detected at higher levels in all strains except SAMN28661660. All L. monocytogenes strains also carried four antibiotic resistance genes (fosX, lin, norB, mprF), contributing to their ability to withstand multiple antimicrobial agents. Notably, no plasmids or mobile genetic elements were detected. Stress resistance genes, including stress survival islet 1 (SSI1_lmo0447), lmo 1800, and lmo1799, were identified in all strains. However, genes encoding for disinfectant resistance were not identified from all strains. LGI-2 was found in all the strains and none of the studied strains harbored LGI-1 and LGI-3. Conserved CRISPR-Cas systems were found in some strains. KEGG pathway analysis revealed that inlA and inlB genes facilitate bacterial internalization through host actin polymerization. Overall, the study provided crucial insights into the genomic features of L. monocytogenes in the Ethiopian dairy chain. It is crucial to establish continuous monitoring of L. monocytogenes in dairy products, improve sanitation, enforce stricter antibiotic usage and food safety regulations, and raise public awareness of associated risks.https://www.frontiersin.org/articles/10.3389/fbinf.2025.1572241/fullantibiotic resistance genesdairy productsListeria monocytogenesmobile genetic elementsvirulence genes |
| spellingShingle | Mebrie Zemene Kinde Bizuayehu Kerisew Tegegne Eshetu Abebe Tesfaye Gessese Genomic analysis of Listeria monocytogenes strains from dairy products in Ethiopia Frontiers in Bioinformatics antibiotic resistance genes dairy products Listeria monocytogenes mobile genetic elements virulence genes |
| title | Genomic analysis of Listeria monocytogenes strains from dairy products in Ethiopia |
| title_full | Genomic analysis of Listeria monocytogenes strains from dairy products in Ethiopia |
| title_fullStr | Genomic analysis of Listeria monocytogenes strains from dairy products in Ethiopia |
| title_full_unstemmed | Genomic analysis of Listeria monocytogenes strains from dairy products in Ethiopia |
| title_short | Genomic analysis of Listeria monocytogenes strains from dairy products in Ethiopia |
| title_sort | genomic analysis of listeria monocytogenes strains from dairy products in ethiopia |
| topic | antibiotic resistance genes dairy products Listeria monocytogenes mobile genetic elements virulence genes |
| url | https://www.frontiersin.org/articles/10.3389/fbinf.2025.1572241/full |
| work_keys_str_mv | AT mebriezemenekinde genomicanalysisoflisteriamonocytogenesstrainsfromdairyproductsinethiopia AT bizuayehukerisew genomicanalysisoflisteriamonocytogenesstrainsfromdairyproductsinethiopia AT tegegneeshetu genomicanalysisoflisteriamonocytogenesstrainsfromdairyproductsinethiopia AT abebetesfayegessese genomicanalysisoflisteriamonocytogenesstrainsfromdairyproductsinethiopia |