CD14 Mediates Binding of High Doses of LPS but Is Dispensable for TNF-α Production
Activation of macrophages with lipopolysaccharide (LPS) involves a sequential engagement of serum LPS-binding protein (LBP), plasma membrane CD14, and TLR4/MD-2 signaling complex. We analyzed participation of CD14 in TNF-α production stimulated with 1–1000 ng/mL of smooth or rough LPS (sLPS or rLPS)...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/824919 |
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| author | Kinga Borzęcka Agnieszka Płóciennikowska Hanna Björkelund Andrzej Sobota Katarzyna Kwiatkowska |
| author_facet | Kinga Borzęcka Agnieszka Płóciennikowska Hanna Björkelund Andrzej Sobota Katarzyna Kwiatkowska |
| author_sort | Kinga Borzęcka |
| collection | DOAJ |
| description | Activation of macrophages with lipopolysaccharide (LPS) involves a sequential engagement of serum LPS-binding protein (LBP), plasma membrane CD14, and TLR4/MD-2 signaling complex. We analyzed participation of CD14 in TNF-α production stimulated with 1–1000 ng/mL of smooth or rough LPS (sLPS or rLPS) and in sLPS binding to RAW264 and J744 cells. CD14 was indispensable for TNF-α generation induced by a low concentration, 1 ng/mL, of sLPS and rLPS. At higher doses of both LPS forms (100–1000 ng/mL), TNF-α release required CD14 to much lower extent. Among the two forms of LPS, rLPS-induced TNF-α production was less CD14-dependent and could proceed in the absence of serum as an LBP source. On the other hand, the involvement of CD14 was crucial for the binding of 1000 ng/mL of sLPS judging from an inhibitory effect of the anti-CD14 antibody. The binding of sLPS was also strongly inhibited by dextran sulfate, a competitive ligand of scavenger receptors (SR). In the presence of dextran sulfate, sLPS-induced production of TNF-α was upregulated about 1.6-fold. The data indicate that CD14 together with SR participates in the binding of high doses of sLPS. However, CD14 contribution to TNF-α production induced by high concentrations of sLPS and rLPS can be limited. |
| format | Article |
| id | doaj-art-24c138c64e554cb4a3b9ca555176c848 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-24c138c64e554cb4a3b9ca555176c8482025-08-20T03:54:56ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/824919824919CD14 Mediates Binding of High Doses of LPS but Is Dispensable for TNF-α ProductionKinga Borzęcka0Agnieszka Płóciennikowska1Hanna Björkelund2Andrzej Sobota3Katarzyna Kwiatkowska4Department of Cell Biology, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, PolandDepartment of Cell Biology, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, PolandRidgeview Instruments AB, Skillsta 4, 740 20 Vänge, SwedenDepartment of Cell Biology, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, PolandDepartment of Cell Biology, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, PolandActivation of macrophages with lipopolysaccharide (LPS) involves a sequential engagement of serum LPS-binding protein (LBP), plasma membrane CD14, and TLR4/MD-2 signaling complex. We analyzed participation of CD14 in TNF-α production stimulated with 1–1000 ng/mL of smooth or rough LPS (sLPS or rLPS) and in sLPS binding to RAW264 and J744 cells. CD14 was indispensable for TNF-α generation induced by a low concentration, 1 ng/mL, of sLPS and rLPS. At higher doses of both LPS forms (100–1000 ng/mL), TNF-α release required CD14 to much lower extent. Among the two forms of LPS, rLPS-induced TNF-α production was less CD14-dependent and could proceed in the absence of serum as an LBP source. On the other hand, the involvement of CD14 was crucial for the binding of 1000 ng/mL of sLPS judging from an inhibitory effect of the anti-CD14 antibody. The binding of sLPS was also strongly inhibited by dextran sulfate, a competitive ligand of scavenger receptors (SR). In the presence of dextran sulfate, sLPS-induced production of TNF-α was upregulated about 1.6-fold. The data indicate that CD14 together with SR participates in the binding of high doses of sLPS. However, CD14 contribution to TNF-α production induced by high concentrations of sLPS and rLPS can be limited.http://dx.doi.org/10.1155/2013/824919 |
| spellingShingle | Kinga Borzęcka Agnieszka Płóciennikowska Hanna Björkelund Andrzej Sobota Katarzyna Kwiatkowska CD14 Mediates Binding of High Doses of LPS but Is Dispensable for TNF-α Production Mediators of Inflammation |
| title | CD14 Mediates Binding of High Doses of LPS but Is Dispensable for TNF-α Production |
| title_full | CD14 Mediates Binding of High Doses of LPS but Is Dispensable for TNF-α Production |
| title_fullStr | CD14 Mediates Binding of High Doses of LPS but Is Dispensable for TNF-α Production |
| title_full_unstemmed | CD14 Mediates Binding of High Doses of LPS but Is Dispensable for TNF-α Production |
| title_short | CD14 Mediates Binding of High Doses of LPS but Is Dispensable for TNF-α Production |
| title_sort | cd14 mediates binding of high doses of lps but is dispensable for tnf α production |
| url | http://dx.doi.org/10.1155/2013/824919 |
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