Acute Phase Protein Response and Polymorphonuclear Leukocyte Cathepsin G Release After Slow Interleukin-1 Stimulation in the Rat
In this work we have studied the acute phase protein response and degranulation of polymorphonuclear leukocytes in vivo in the rat after a slow interleukin-1β stimulation. A total dose of 1 μg, 2 μg, 4 μg and 0 μg (controls with only vehicle) of interleukin-1β was released from osmotic minipumps ove...
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| Format: | Article |
| Language: | English |
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Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000608 |
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| _version_ | 1850228024328323072 |
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| author | Peter Björk Thorarinn Gudmundsson Kjell Ohlsson |
| author_facet | Peter Björk Thorarinn Gudmundsson Kjell Ohlsson |
| author_sort | Peter Björk |
| collection | DOAJ |
| description | In this work we have studied the acute phase protein response and
degranulation of polymorphonuclear leukocytes in vivo in the rat
after a slow interleukin-1β stimulation. A total dose of 1 μg, 2 μg,
4 μg and 0 μg (controls with only vehicle) of interleukin-1β was
released from osmotic minipumps over a period of 7 days. The pumps
were implanted subcutaneously. A cystic formation was formed around
the pumps that contained interleukin-1β whereas no tissue reaction
was seen around pumps containing only vehicle. Besides flbroblasts
the cyst wall contained numerous polymorphonuclear leukocytes which
were positively stained for cathespin G. α2-macroglobulin,
α1-inhtbitor-3, α1-proteinase inhibitor, albumin and C3 were
measured by electroimmunoassay and all showed plasma concentration
patterns that were dose-dependent to the amount of interleuktn-1β
released. Fibrinogen in plasma was elevated in the control group but
showed decreased plasma values with higher doses of interleukin-1β
released. All animals showed increased plasma levels of cathespin G
but the lowest levels for cathespin G were seen for the highest
interleukin-1β dose released. It was clearly seen that a slow
continuous release of interleukin-1β in vivo caused an inflammatory
reaction. Plasma levels for the proteins analysed all showed a
similar pattern, namely an initial increase or decrease of plasma
concentration followed by a tendency to normalization of plasma
values. It was concluded that a long-term interleukin-1β release
could not sustain the acute phase protein response elicited by the
initial interleukin-1β release. |
| format | Article |
| id | doaj-art-24aa725a0f3244dfb7e6f007d2bef116 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1994-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-24aa725a0f3244dfb7e6f007d2bef1162025-08-20T02:04:40ZengWileyMediators of Inflammation0962-93511466-18611994-01-013642543110.1155/S0962935194000608Acute Phase Protein Response and Polymorphonuclear Leukocyte Cathepsin G Release After Slow Interleukin-1 Stimulation in the RatPeter Björk0Thorarinn Gudmundsson1Kjell Ohlsson2Department of Surgical Pathophysiology, University of Lund, Malmö General Hospital, Malmö S-214 01, SwedenDepartment of Surgical Pathophysiology, University of Lund, Malmö General Hospital, Malmö S-214 01, SwedenDepartment of Surgical Pathophysiology, University of Lund, Malmö General Hospital, Malmö S-214 01, SwedenIn this work we have studied the acute phase protein response and degranulation of polymorphonuclear leukocytes in vivo in the rat after a slow interleukin-1β stimulation. A total dose of 1 μg, 2 μg, 4 μg and 0 μg (controls with only vehicle) of interleukin-1β was released from osmotic minipumps over a period of 7 days. The pumps were implanted subcutaneously. A cystic formation was formed around the pumps that contained interleukin-1β whereas no tissue reaction was seen around pumps containing only vehicle. Besides flbroblasts the cyst wall contained numerous polymorphonuclear leukocytes which were positively stained for cathespin G. α2-macroglobulin, α1-inhtbitor-3, α1-proteinase inhibitor, albumin and C3 were measured by electroimmunoassay and all showed plasma concentration patterns that were dose-dependent to the amount of interleuktn-1β released. Fibrinogen in plasma was elevated in the control group but showed decreased plasma values with higher doses of interleukin-1β released. All animals showed increased plasma levels of cathespin G but the lowest levels for cathespin G were seen for the highest interleukin-1β dose released. It was clearly seen that a slow continuous release of interleukin-1β in vivo caused an inflammatory reaction. Plasma levels for the proteins analysed all showed a similar pattern, namely an initial increase or decrease of plasma concentration followed by a tendency to normalization of plasma values. It was concluded that a long-term interleukin-1β release could not sustain the acute phase protein response elicited by the initial interleukin-1β release.http://dx.doi.org/10.1155/S0962935194000608 |
| spellingShingle | Peter Björk Thorarinn Gudmundsson Kjell Ohlsson Acute Phase Protein Response and Polymorphonuclear Leukocyte Cathepsin G Release After Slow Interleukin-1 Stimulation in the Rat Mediators of Inflammation |
| title | Acute Phase Protein Response and Polymorphonuclear Leukocyte
Cathepsin G Release After Slow Interleukin-1 Stimulation in the Rat |
| title_full | Acute Phase Protein Response and Polymorphonuclear Leukocyte
Cathepsin G Release After Slow Interleukin-1 Stimulation in the Rat |
| title_fullStr | Acute Phase Protein Response and Polymorphonuclear Leukocyte
Cathepsin G Release After Slow Interleukin-1 Stimulation in the Rat |
| title_full_unstemmed | Acute Phase Protein Response and Polymorphonuclear Leukocyte
Cathepsin G Release After Slow Interleukin-1 Stimulation in the Rat |
| title_short | Acute Phase Protein Response and Polymorphonuclear Leukocyte
Cathepsin G Release After Slow Interleukin-1 Stimulation in the Rat |
| title_sort | acute phase protein response and polymorphonuclear leukocyte cathepsin g release after slow interleukin 1 stimulation in the rat |
| url | http://dx.doi.org/10.1155/S0962935194000608 |
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