Immune and Angiogenic Profiling of Mesenchymal Stem Cell Functions in a Subcutaneous Microenvironment for Allogeneic Islet Transplantation

Abstract Islet transplantation offers a promising treatment for type 1 diabetes (T1D), by aiming to restore insulin production and improve glycemic control. However, T1D is compounded by impaired angiogenesis and immune dysregulation, which hinder the therapeutic potential of cell replacement strate...

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Main Authors: Jocelyn Nikita Campa‐Carranza, Simone Capuani, Ashley L. Joubert, Nathanael Hernandez, Tommaso Bo, Octavio I. Sauceda‐Villanueva, Marzia Conte, Letizia Franco, Marco Farina, Gabrielle E. Rome, Yitian Xu, Junjun Zheng, Lissenya B. Argueta, Jean A. Niles, Fotis Nikolos, Corrine Ying Xuan Chua, Shu‐Hsia Chen, Joan E. Nichols, Norma S. Kenyon, Alessandro Grattoni
Format: Article
Language:English
Published: Wiley 2025-05-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202411574
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author Jocelyn Nikita Campa‐Carranza
Simone Capuani
Ashley L. Joubert
Nathanael Hernandez
Tommaso Bo
Octavio I. Sauceda‐Villanueva
Marzia Conte
Letizia Franco
Marco Farina
Gabrielle E. Rome
Yitian Xu
Junjun Zheng
Lissenya B. Argueta
Jean A. Niles
Fotis Nikolos
Corrine Ying Xuan Chua
Shu‐Hsia Chen
Joan E. Nichols
Norma S. Kenyon
Alessandro Grattoni
author_facet Jocelyn Nikita Campa‐Carranza
Simone Capuani
Ashley L. Joubert
Nathanael Hernandez
Tommaso Bo
Octavio I. Sauceda‐Villanueva
Marzia Conte
Letizia Franco
Marco Farina
Gabrielle E. Rome
Yitian Xu
Junjun Zheng
Lissenya B. Argueta
Jean A. Niles
Fotis Nikolos
Corrine Ying Xuan Chua
Shu‐Hsia Chen
Joan E. Nichols
Norma S. Kenyon
Alessandro Grattoni
author_sort Jocelyn Nikita Campa‐Carranza
collection DOAJ
description Abstract Islet transplantation offers a promising treatment for type 1 diabetes (T1D), by aiming to restore insulin production and improve glycemic control. However, T1D is compounded by impaired angiogenesis and immune dysregulation, which hinder the therapeutic potential of cell replacement strategies. To address this, this work evaluates the proangiogenic and immunomodulatory properties of mesenchymal stem cells (MSCs) to enhance vascularization and modulate early‐stage immune rejection pathways in the context of islet allotransplantation. This work employs the Neovascularized Implantable Cell Homing and Encapsulation (NICHE) platform, a subcutaneous vascularized implant with localized immunomodulation developed by the group. This study assesses vascularization and immune regulation provided by MSCs, aiming to improve islet survival and integration in diabetic rats while considering sex as a biological variable. These findings demonstrate that MSCs significantly enhance vascularization and modulate the local microenvironment during the peri‐transplant period. Importantly, this work discovers sex‐specific differences in both processes, which influence islet engraftment and long‐term function.
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spelling doaj-art-249b7bb4be3b4141a1e27da1133712d42025-08-20T02:34:43ZengWileyAdvanced Science2198-38442025-05-011220n/an/a10.1002/advs.202411574Immune and Angiogenic Profiling of Mesenchymal Stem Cell Functions in a Subcutaneous Microenvironment for Allogeneic Islet TransplantationJocelyn Nikita Campa‐Carranza0Simone Capuani1Ashley L. Joubert2Nathanael Hernandez3Tommaso Bo4Octavio I. Sauceda‐Villanueva5Marzia Conte6Letizia Franco7Marco Farina8Gabrielle E. Rome9Yitian Xu10Junjun Zheng11Lissenya B. Argueta12Jean A. Niles13Fotis Nikolos14Corrine Ying Xuan Chua15Shu‐Hsia Chen16Joan E. Nichols17Norma S. Kenyon18Alessandro Grattoni19Department of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USADepartment of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USADepartment of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USADepartment of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USADepartment of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USADepartment of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USADepartment of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USADepartment of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USADepartment of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USADepartment of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USACenter for Immunotherapy Research Houston Methodist Research Institute Houston TX 77030 USACenter for Immunotherapy Research Houston Methodist Research Institute Houston TX 77030 USACenter for Tissue Engineering Houston Methodist Research Institute Houston TX 77030 USACenter for Tissue Engineering Houston Methodist Research Institute Houston TX 77030 USADepartment of Urology Houston Methodist Research Institute Houston TX 77030 USADepartment of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USACenter for Immunotherapy Research Houston Methodist Research Institute Houston TX 77030 USACenter for Tissue Engineering Houston Methodist Research Institute Houston TX 77030 USADiabetes Research Institute University of Miami Miami FL 33136 USADepartment of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USAAbstract Islet transplantation offers a promising treatment for type 1 diabetes (T1D), by aiming to restore insulin production and improve glycemic control. However, T1D is compounded by impaired angiogenesis and immune dysregulation, which hinder the therapeutic potential of cell replacement strategies. To address this, this work evaluates the proangiogenic and immunomodulatory properties of mesenchymal stem cells (MSCs) to enhance vascularization and modulate early‐stage immune rejection pathways in the context of islet allotransplantation. This work employs the Neovascularized Implantable Cell Homing and Encapsulation (NICHE) platform, a subcutaneous vascularized implant with localized immunomodulation developed by the group. This study assesses vascularization and immune regulation provided by MSCs, aiming to improve islet survival and integration in diabetic rats while considering sex as a biological variable. These findings demonstrate that MSCs significantly enhance vascularization and modulate the local microenvironment during the peri‐transplant period. Importantly, this work discovers sex‐specific differences in both processes, which influence islet engraftment and long‐term function.https://doi.org/10.1002/advs.202411574immunomodulationislet engraftmentmesenchymal stem cellssex‐specific differencesType 1 diabetesvascularized subcutaneous microenvironment
spellingShingle Jocelyn Nikita Campa‐Carranza
Simone Capuani
Ashley L. Joubert
Nathanael Hernandez
Tommaso Bo
Octavio I. Sauceda‐Villanueva
Marzia Conte
Letizia Franco
Marco Farina
Gabrielle E. Rome
Yitian Xu
Junjun Zheng
Lissenya B. Argueta
Jean A. Niles
Fotis Nikolos
Corrine Ying Xuan Chua
Shu‐Hsia Chen
Joan E. Nichols
Norma S. Kenyon
Alessandro Grattoni
Immune and Angiogenic Profiling of Mesenchymal Stem Cell Functions in a Subcutaneous Microenvironment for Allogeneic Islet Transplantation
Advanced Science
immunomodulation
islet engraftment
mesenchymal stem cells
sex‐specific differences
Type 1 diabetes
vascularized subcutaneous microenvironment
title Immune and Angiogenic Profiling of Mesenchymal Stem Cell Functions in a Subcutaneous Microenvironment for Allogeneic Islet Transplantation
title_full Immune and Angiogenic Profiling of Mesenchymal Stem Cell Functions in a Subcutaneous Microenvironment for Allogeneic Islet Transplantation
title_fullStr Immune and Angiogenic Profiling of Mesenchymal Stem Cell Functions in a Subcutaneous Microenvironment for Allogeneic Islet Transplantation
title_full_unstemmed Immune and Angiogenic Profiling of Mesenchymal Stem Cell Functions in a Subcutaneous Microenvironment for Allogeneic Islet Transplantation
title_short Immune and Angiogenic Profiling of Mesenchymal Stem Cell Functions in a Subcutaneous Microenvironment for Allogeneic Islet Transplantation
title_sort immune and angiogenic profiling of mesenchymal stem cell functions in a subcutaneous microenvironment for allogeneic islet transplantation
topic immunomodulation
islet engraftment
mesenchymal stem cells
sex‐specific differences
Type 1 diabetes
vascularized subcutaneous microenvironment
url https://doi.org/10.1002/advs.202411574
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