Determination of Multiple Free Nucleotides in Meat Products by High Performance Liquid Chromatography
This study developed and validated a method to detect adenosine monophosphate (AMP), inosine monphosphate (IMP), guanosine monophosphate (GMP), hypoxanthine riboside (HxR), and hypoxanthine (Hx) in meat products by high performance liquid chromatography (HPLC). The optimized procedure was as follows...
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China Food Publishing Company
2025-07-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-13-033.pdf |
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| author | JI Xiaomeng, WANG Zhen, REN Nan, LIU Mengyao, KONG Weiheng, ZHAO Wentao, WANG Jiangyue, LIU Xinyi, GUO Wenping |
| author_facet | JI Xiaomeng, WANG Zhen, REN Nan, LIU Mengyao, KONG Weiheng, ZHAO Wentao, WANG Jiangyue, LIU Xinyi, GUO Wenping |
| author_sort | JI Xiaomeng, WANG Zhen, REN Nan, LIU Mengyao, KONG Weiheng, ZHAO Wentao, WANG Jiangyue, LIU Xinyi, GUO Wenping |
| collection | DOAJ |
| description | This study developed and validated a method to detect adenosine monophosphate (AMP), inosine monphosphate (IMP), guanosine monophosphate (GMP), hypoxanthine riboside (HxR), and hypoxanthine (Hx) in meat products by high performance liquid chromatography (HPLC). The optimized procedure was as follows: samples were extracted twice with 5% perchloric acid with shaking for 30 min; the pooled extract was adjusted to pH 6.5 and made up to 50 mL with deionized water; an appropriate amount of the extract was taken and purified by liquid-liquid extraction with n-hexane followed by centrifugation; the bottom layer was harvested for analysis by HPLC. Chromatographic separation was accomplished on a C18 column using isocratic elution with a mobile phase composed of 0.01 mol/L aqueous potassium dihydrogen phosphate and methanol (98:2, V/V) at a flow rate 1.0 mL/min, and injection volume was set at 20 μL. Detection wavelength was set at 254 nm. Quantification was carried out using the external standard method. The results showed that the five nucleosides had a good linear relationship within the concentration range of 0.5–80 μg/mL, with correlation coefficients greater than 0.999. The limit of detection (LOD) was 5.0 mg/kg, the limit of quantitation (LOQ) was 15.0 mg/kg. Recoveries from spiked samples was 80.1%–109.5% with relative standard deviation (RSD) ≤ 9.6% (n = 3). In summary, this method is simple to operate, accurate, repeatable, and suitable for the detection of the five free nucleotides in the complex meat matrix. |
| format | Article |
| id | doaj-art-24840f5d3ffb4ea5a254d60180912b96 |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-24840f5d3ffb4ea5a254d60180912b962025-08-20T03:51:08ZengChina Food Publishing CompanyShipin Kexue1002-66302025-07-01461334129610.7506/spkx1002-6630-20241209-060Determination of Multiple Free Nucleotides in Meat Products by High Performance Liquid ChromatographyJI Xiaomeng, WANG Zhen, REN Nan, LIU Mengyao, KONG Weiheng, ZHAO Wentao, WANG Jiangyue, LIU Xinyi, GUO Wenping0(1. China Meat Research Center, Beijing 100068, China; 2. Science and Technology Research Center of China Customs, Beijing 100026, China)This study developed and validated a method to detect adenosine monophosphate (AMP), inosine monphosphate (IMP), guanosine monophosphate (GMP), hypoxanthine riboside (HxR), and hypoxanthine (Hx) in meat products by high performance liquid chromatography (HPLC). The optimized procedure was as follows: samples were extracted twice with 5% perchloric acid with shaking for 30 min; the pooled extract was adjusted to pH 6.5 and made up to 50 mL with deionized water; an appropriate amount of the extract was taken and purified by liquid-liquid extraction with n-hexane followed by centrifugation; the bottom layer was harvested for analysis by HPLC. Chromatographic separation was accomplished on a C18 column using isocratic elution with a mobile phase composed of 0.01 mol/L aqueous potassium dihydrogen phosphate and methanol (98:2, V/V) at a flow rate 1.0 mL/min, and injection volume was set at 20 μL. Detection wavelength was set at 254 nm. Quantification was carried out using the external standard method. The results showed that the five nucleosides had a good linear relationship within the concentration range of 0.5–80 μg/mL, with correlation coefficients greater than 0.999. The limit of detection (LOD) was 5.0 mg/kg, the limit of quantitation (LOQ) was 15.0 mg/kg. Recoveries from spiked samples was 80.1%–109.5% with relative standard deviation (RSD) ≤ 9.6% (n = 3). In summary, this method is simple to operate, accurate, repeatable, and suitable for the detection of the five free nucleotides in the complex meat matrix.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-13-033.pdfhigh performance liquid chromatography; meat products; adenosine monophosphate; guanosine monophosphate; inosine monphosphate; hypoxanthine riboside; hypoxanthine |
| spellingShingle | JI Xiaomeng, WANG Zhen, REN Nan, LIU Mengyao, KONG Weiheng, ZHAO Wentao, WANG Jiangyue, LIU Xinyi, GUO Wenping Determination of Multiple Free Nucleotides in Meat Products by High Performance Liquid Chromatography Shipin Kexue high performance liquid chromatography; meat products; adenosine monophosphate; guanosine monophosphate; inosine monphosphate; hypoxanthine riboside; hypoxanthine |
| title | Determination of Multiple Free Nucleotides in Meat Products by High Performance Liquid Chromatography |
| title_full | Determination of Multiple Free Nucleotides in Meat Products by High Performance Liquid Chromatography |
| title_fullStr | Determination of Multiple Free Nucleotides in Meat Products by High Performance Liquid Chromatography |
| title_full_unstemmed | Determination of Multiple Free Nucleotides in Meat Products by High Performance Liquid Chromatography |
| title_short | Determination of Multiple Free Nucleotides in Meat Products by High Performance Liquid Chromatography |
| title_sort | determination of multiple free nucleotides in meat products by high performance liquid chromatography |
| topic | high performance liquid chromatography; meat products; adenosine monophosphate; guanosine monophosphate; inosine monphosphate; hypoxanthine riboside; hypoxanthine |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-13-033.pdf |
| work_keys_str_mv | AT jixiaomengwangzhenrennanliumengyaokongweihengzhaowentaowangjiangyueliuxinyiguowenping determinationofmultiplefreenucleotidesinmeatproductsbyhighperformanceliquidchromatography |