Bio-based Superhydrophobic Sensor for Enhanced Anti-Fouling and Rapid Bacterial Detection in Hematology

Hematology patients are at high risk of nosocomial infections, necessitating rapid and reliable bacterial detection. Current methods, such as culture and polymerase chain reaction (PCR), are often slow, expensive, or susceptible to contamination. This research introduces a novel bio-based superhydro...

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Main Authors: Lifen LAN, Xiaoxiao LU
Format: Article
Language:English
Published: Kaunas University of Technology 2025-06-01
Series:Medžiagotyra
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Online Access:https://matsc.ktu.lt/index.php/MatSc/article/view/40980
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author Lifen LAN
Xiaoxiao LU
author_facet Lifen LAN
Xiaoxiao LU
author_sort Lifen LAN
collection DOAJ
description Hematology patients are at high risk of nosocomial infections, necessitating rapid and reliable bacterial detection. Current methods, such as culture and polymerase chain reaction (PCR), are often slow, expensive, or susceptible to contamination. This research introduces a novel bio-based superhydrophobic sensor to address these limitations. The sensor utilizes a polydimethylsiloxane (PDMS) and TiO2 coated substrate to create a superhydrophobic surface (water contact angle >150°), which significantly reduces bacterial adhesion. Detection relies on peptides that undergo a conformational change upon binding specific bacteria, releasing attached magnetic beads. These beads are detected electrochemically using an indium tin oxide (ITO) electrode modified with cadmium telluride (CdTe) quantum dots and graphene oxide (GO) composite, enhancing electron transfer efficiency by 2.3-fold compared with bare ITO. This design achieved rapid (<90 minutes) and sensitive detection. The sensor had high accuracy comparable to PCR and discriminated between Klebsiella pneumoniae (K. pneumoniae) and Pseudomonas aeruginosa (P. aeruginosa). By combining anti-fouling properties with specific bacterial recognition and enhanced electrochemical detection, this sensor offers a promising platform for reducing infection risks in hematology.
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institution Kabale University
issn 1392-1320
2029-7289
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publishDate 2025-06-01
publisher Kaunas University of Technology
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spelling doaj-art-246d64d30f64407983f5961fd6efea9d2025-08-20T03:25:15ZengKaunas University of TechnologyMedžiagotyra1392-13202029-72892025-06-0110.5755/j02.ms.4098046234Bio-based Superhydrophobic Sensor for Enhanced Anti-Fouling and Rapid Bacterial Detection in HematologyLifen LAN0https://orcid.org/0009-0008-0478-1015Xiaoxiao LU1https://orcid.org/0009-0000-1088-5293Affiliated Hospital of Inner Mongolia Medical UniversityChongqing University Cancer HospitalHematology patients are at high risk of nosocomial infections, necessitating rapid and reliable bacterial detection. Current methods, such as culture and polymerase chain reaction (PCR), are often slow, expensive, or susceptible to contamination. This research introduces a novel bio-based superhydrophobic sensor to address these limitations. The sensor utilizes a polydimethylsiloxane (PDMS) and TiO2 coated substrate to create a superhydrophobic surface (water contact angle >150°), which significantly reduces bacterial adhesion. Detection relies on peptides that undergo a conformational change upon binding specific bacteria, releasing attached magnetic beads. These beads are detected electrochemically using an indium tin oxide (ITO) electrode modified with cadmium telluride (CdTe) quantum dots and graphene oxide (GO) composite, enhancing electron transfer efficiency by 2.3-fold compared with bare ITO. This design achieved rapid (<90 minutes) and sensitive detection. The sensor had high accuracy comparable to PCR and discriminated between Klebsiella pneumoniae (K. pneumoniae) and Pseudomonas aeruginosa (P. aeruginosa). By combining anti-fouling properties with specific bacterial recognition and enhanced electrochemical detection, this sensor offers a promising platform for reducing infection risks in hematology.https://matsc.ktu.lt/index.php/MatSc/article/view/40980bio-basedsuperhydrophobichematologyanti-foulingbacterial detectionelectrochemical sensorpeptidequantum dots
spellingShingle Lifen LAN
Xiaoxiao LU
Bio-based Superhydrophobic Sensor for Enhanced Anti-Fouling and Rapid Bacterial Detection in Hematology
Medžiagotyra
bio-based
superhydrophobic
hematology
anti-fouling
bacterial detection
electrochemical sensor
peptide
quantum dots
title Bio-based Superhydrophobic Sensor for Enhanced Anti-Fouling and Rapid Bacterial Detection in Hematology
title_full Bio-based Superhydrophobic Sensor for Enhanced Anti-Fouling and Rapid Bacterial Detection in Hematology
title_fullStr Bio-based Superhydrophobic Sensor for Enhanced Anti-Fouling and Rapid Bacterial Detection in Hematology
title_full_unstemmed Bio-based Superhydrophobic Sensor for Enhanced Anti-Fouling and Rapid Bacterial Detection in Hematology
title_short Bio-based Superhydrophobic Sensor for Enhanced Anti-Fouling and Rapid Bacterial Detection in Hematology
title_sort bio based superhydrophobic sensor for enhanced anti fouling and rapid bacterial detection in hematology
topic bio-based
superhydrophobic
hematology
anti-fouling
bacterial detection
electrochemical sensor
peptide
quantum dots
url https://matsc.ktu.lt/index.php/MatSc/article/view/40980
work_keys_str_mv AT lifenlan biobasedsuperhydrophobicsensorforenhancedantifoulingandrapidbacterialdetectioninhematology
AT xiaoxiaolu biobasedsuperhydrophobicsensorforenhancedantifoulingandrapidbacterialdetectioninhematology