Biocatalytic Potential of a <i>Raoultella terrigena</i>-Derived Lipolytic Enzyme for High-Performance Detergents
Dump sites harbour microorganisms with potential for environmentally friendly industrial applications. This study assessed the lipolytic activity of municipal dumpsite-associated bacteria and evaluated the stability of the most potent isolate’s lipolytic enzyme against laundry detergents. It also ex...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/4/225 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Dump sites harbour microorganisms with potential for environmentally friendly industrial applications. This study assessed the lipolytic activity of municipal dumpsite-associated bacteria and evaluated the stability of the most potent isolate’s lipolytic enzyme against laundry detergents. It also examined the crude lipase’s ability to remove stains from cotton fabric. Among twelve bacteria isolated, five demonstrated notable halo zones on tributyrin agar plates. The diameters (mm) were MN38 (11 ± 1.4), MN1310 (8.5 ± 0.7), MN28 (6.5 ± 0.71), MN18 (7.0 ± 1.4), and MN310 (8.15 ± 0.21). Quantitative analysis revealed that MN38 exhibited the highest lipase activity (14.76 ± 0.27 U/mL), while MN1310 showed the lowest (6.40 ± 0.85 U/mL). Nucleotide sequence analysis identified the isolates as <i>Raoultella terrigena</i> veli18 (MN38), <i>Stenotrophomonas maltophilia</i> veli96 (MN1310), <i>Viridibacillus</i> sp. veli10 (MN28), <i>Stenotrophomonas</i> sp. veli19 (MN18), and <i>Klebsiella</i> sp. veli70 (MN310). The crude lipase from <i>R. terrigena</i> veli18 maintained 73.33%, 52.67%, 55.0%, and 54.0% of its original activity after 60 min of exposure to Sunlight, Surf, Maq, and Omo, respectively. Adding crude lipase to enzyme-free laundry detergents significantly enhanced their cleaning efficacy, completely removing oil stains from cotton fabric. This performance of <i>R. terrigena</i> veli18 crude lipase highlights its potential as an effective detergent bio-additive. |
|---|---|
| ISSN: | 2311-5637 |