E-cadherin inhibits the proliferation and migration of human colorectal cancer cells through Hippo signaling pathway
E-cadherin (E-cad) is a crucial regulatory factor in rescue Epithelial-mesenchymal transition and is involved in the occurrence of various malignant tumor. However, the mechanisms by which E-cadherin regulates tumor metastasis in CRC remain unclear. We established sh-E-cad (silenced by short hairpi...
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| Format: | Article |
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PAGEPress Publications
2025-05-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | https://www.ejh.it/ejh/article/view/4196 |
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| author | Zhijing Wang Xiaohua Qin Shanshan Liu Yilei Wen Bikan Lan Hantao Liao Haixian Wei |
| author_facet | Zhijing Wang Xiaohua Qin Shanshan Liu Yilei Wen Bikan Lan Hantao Liao Haixian Wei |
| author_sort | Zhijing Wang |
| collection | DOAJ |
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E-cadherin (E-cad) is a crucial regulatory factor in rescue Epithelial-mesenchymal transition and is involved in the occurrence of various malignant tumor. However, the mechanisms by which E-cadherin regulates tumor metastasis in CRC remain unclear. We established sh-E-cad (silenced by short hairpin RNA) and rescue-E-cad (overexpressed by E-cad plasmid transfection) CRC cell lines to investigate the role of E-cad in CRC in vitro. Immunohistochemistry, clonogenic assays, scratch wound healing assays, CCK-8 assays, flow cytometry, Transwell assay, real time-PCR and Western blot were employed to investigate the underlying mechanisms by which E-cad involve the progression of CRC. In CRC tissues, E-cad expression was significantly reduced, while YAP expression was markedly elevated. Silencing E-cad induced a significant increase of clonogenic ability in CRC cells, which was reduced upon rescue of E-cad expression. Transwell assays indicate that low expression of E-cad enhances the cell migration, a finding corroborated by scratch wound healing experiments. CCK-8 results demonstrate that silencing E-cad promotes the proliferation of CRC cells. Importantly, we found that E-cad influences apoptosis rather than the cell cycle. Analysis of Hippo signaling pathway-related factors revealed that silencing E-cad resulted in significantly decreased expression of MST1/2 and LATS1/2, as well as reduced phosphorylation levels of YAP, while YAP expression was significantly increased. Additionally, immunofluorescence confirmed the nuclear translocation of YAP. Our study indicates that E-cad regulates the malignant progression of CRC via the Hippo signaling pathway, offering a potential new strategy for CRC treatment.
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| format | Article |
| id | doaj-art-2463c7f53aa149ec82abc60ecea6c437 |
| institution | OA Journals |
| issn | 1121-760X 2038-8306 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj-art-2463c7f53aa149ec82abc60ecea6c4372025-08-20T02:30:00ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062025-05-0169210.4081/ejh.2025.4196E-cadherin inhibits the proliferation and migration of human colorectal cancer cells through Hippo signaling pathwayZhijing Wang0Xiaohua Qin1Shanshan Liu2Yilei Wen3Bikan Lan4Hantao Liao5Haixian Wei6Department of Pathology, The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Qingxiu District, Nanning City, Guangxi Zhuang Autonomous RegionDepartment of Pathology, The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Qingxiu District, Nanning City, Guangxi Zhuang Autonomous RegionDepartment of Pathology, The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Qingxiu District, Nanning City, Guangxi Zhuang Autonomous RegionDepartment of Pathology, The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Qingxiu District, Nanning City, Guangxi Zhuang Autonomous RegionDepartment of Pathology, The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Qingxiu District, Nanning City, Guangxi Zhuang Autonomous RegionSchool of Basic Science, Guangxi University of Traditional Chinese Medicine, Qingxiu District, Nanning City, Guangxi Zhuang Autonomous RegionSchool of Basic Science, Guangxi University of Traditional Chinese Medicine, Qingxiu District, Nanning City, Guangxi Zhuang Autonomous Region E-cadherin (E-cad) is a crucial regulatory factor in rescue Epithelial-mesenchymal transition and is involved in the occurrence of various malignant tumor. However, the mechanisms by which E-cadherin regulates tumor metastasis in CRC remain unclear. We established sh-E-cad (silenced by short hairpin RNA) and rescue-E-cad (overexpressed by E-cad plasmid transfection) CRC cell lines to investigate the role of E-cad in CRC in vitro. Immunohistochemistry, clonogenic assays, scratch wound healing assays, CCK-8 assays, flow cytometry, Transwell assay, real time-PCR and Western blot were employed to investigate the underlying mechanisms by which E-cad involve the progression of CRC. In CRC tissues, E-cad expression was significantly reduced, while YAP expression was markedly elevated. Silencing E-cad induced a significant increase of clonogenic ability in CRC cells, which was reduced upon rescue of E-cad expression. Transwell assays indicate that low expression of E-cad enhances the cell migration, a finding corroborated by scratch wound healing experiments. CCK-8 results demonstrate that silencing E-cad promotes the proliferation of CRC cells. Importantly, we found that E-cad influences apoptosis rather than the cell cycle. Analysis of Hippo signaling pathway-related factors revealed that silencing E-cad resulted in significantly decreased expression of MST1/2 and LATS1/2, as well as reduced phosphorylation levels of YAP, while YAP expression was significantly increased. Additionally, immunofluorescence confirmed the nuclear translocation of YAP. Our study indicates that E-cad regulates the malignant progression of CRC via the Hippo signaling pathway, offering a potential new strategy for CRC treatment. https://www.ejh.it/ejh/article/view/4196Colorectal cancerE-cadherinHippo signaling pathwayapoptosis |
| spellingShingle | Zhijing Wang Xiaohua Qin Shanshan Liu Yilei Wen Bikan Lan Hantao Liao Haixian Wei E-cadherin inhibits the proliferation and migration of human colorectal cancer cells through Hippo signaling pathway European Journal of Histochemistry Colorectal cancer E-cadherin Hippo signaling pathway apoptosis |
| title | E-cadherin inhibits the proliferation and migration of human colorectal cancer cells through Hippo signaling pathway |
| title_full | E-cadherin inhibits the proliferation and migration of human colorectal cancer cells through Hippo signaling pathway |
| title_fullStr | E-cadherin inhibits the proliferation and migration of human colorectal cancer cells through Hippo signaling pathway |
| title_full_unstemmed | E-cadherin inhibits the proliferation and migration of human colorectal cancer cells through Hippo signaling pathway |
| title_short | E-cadherin inhibits the proliferation and migration of human colorectal cancer cells through Hippo signaling pathway |
| title_sort | e cadherin inhibits the proliferation and migration of human colorectal cancer cells through hippo signaling pathway |
| topic | Colorectal cancer E-cadherin Hippo signaling pathway apoptosis |
| url | https://www.ejh.it/ejh/article/view/4196 |
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