Classical and Modern Models for Biofilm Studies: A Comprehensive Review
Biofilms are structured microbial communities that adhere to various abiotic and biotic surfaces, where organisms are encased in an exo-polysaccharide matrix. Organisms within biofilms use various mechanisms that help them resist external challenges, such as antibiotics, rendering them more resistan...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/13/12/1228 |
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| author | Zhihe Yang Sadaf Aiman Khan Laurence J. Walsh Zyta M. Ziora Chaminda Jayampath Seneviratne |
| author_facet | Zhihe Yang Sadaf Aiman Khan Laurence J. Walsh Zyta M. Ziora Chaminda Jayampath Seneviratne |
| author_sort | Zhihe Yang |
| collection | DOAJ |
| description | Biofilms are structured microbial communities that adhere to various abiotic and biotic surfaces, where organisms are encased in an exo-polysaccharide matrix. Organisms within biofilms use various mechanisms that help them resist external challenges, such as antibiotics, rendering them more resistant to drugs. Therefore, researchers have attempted to develop suitable laboratory models to study the physical features of biofilms, their resistance mechanisms against antimicrobial agents, and their gene and protein expression profiles. However, current laboratory models suffer from various limitations. In this comprehensive review, we have summarized the various designs that have been used for laboratory biofilm models, presenting their strengths and limitations. Additionally, we have provided insight into improving these models to more closely simulate real-life scenarios, using newly developed techniques in additive manufacturing, synthetic biology, and bioengineering. |
| format | Article |
| id | doaj-art-2463a91b851f43ea94e1f9382c8fbd7c |
| institution | DOAJ |
| issn | 2079-6382 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-2463a91b851f43ea94e1f9382c8fbd7c2025-08-20T02:53:41ZengMDPI AGAntibiotics2079-63822024-12-011312122810.3390/antibiotics13121228Classical and Modern Models for Biofilm Studies: A Comprehensive ReviewZhihe Yang0Sadaf Aiman Khan1Laurence J. Walsh2Zyta M. Ziora3Chaminda Jayampath Seneviratne4School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, QLD 4072, AustraliaOral Health Centre, School of Dentistry, University of Queensland, Herston, QLD 4006, AustraliaOral Health Centre, School of Dentistry, University of Queensland, Herston, QLD 4006, AustraliaInstitute for Molecular Bioscience, University of Queensland, St. Lucia, QLD 4072, AustraliaOral Health Centre, School of Dentistry, University of Queensland, Herston, QLD 4006, AustraliaBiofilms are structured microbial communities that adhere to various abiotic and biotic surfaces, where organisms are encased in an exo-polysaccharide matrix. Organisms within biofilms use various mechanisms that help them resist external challenges, such as antibiotics, rendering them more resistant to drugs. Therefore, researchers have attempted to develop suitable laboratory models to study the physical features of biofilms, their resistance mechanisms against antimicrobial agents, and their gene and protein expression profiles. However, current laboratory models suffer from various limitations. In this comprehensive review, we have summarized the various designs that have been used for laboratory biofilm models, presenting their strengths and limitations. Additionally, we have provided insight into improving these models to more closely simulate real-life scenarios, using newly developed techniques in additive manufacturing, synthetic biology, and bioengineering.https://www.mdpi.com/2079-6382/13/12/1228biofilmslaboratory modelsmicro-environments |
| spellingShingle | Zhihe Yang Sadaf Aiman Khan Laurence J. Walsh Zyta M. Ziora Chaminda Jayampath Seneviratne Classical and Modern Models for Biofilm Studies: A Comprehensive Review Antibiotics biofilms laboratory models micro-environments |
| title | Classical and Modern Models for Biofilm Studies: A Comprehensive Review |
| title_full | Classical and Modern Models for Biofilm Studies: A Comprehensive Review |
| title_fullStr | Classical and Modern Models for Biofilm Studies: A Comprehensive Review |
| title_full_unstemmed | Classical and Modern Models for Biofilm Studies: A Comprehensive Review |
| title_short | Classical and Modern Models for Biofilm Studies: A Comprehensive Review |
| title_sort | classical and modern models for biofilm studies a comprehensive review |
| topic | biofilms laboratory models micro-environments |
| url | https://www.mdpi.com/2079-6382/13/12/1228 |
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