Human serum albumin regulates the ornithine carbamoyltransferase production of Streptococcus pyogenes CS24 in vitro
The M12 protein is the main virulence factor of Streptococcus pyogenes as a mediator in the bacteria’s adhesion to numerous human proteins, including human serum albumin (HSA) and immunoglobulin. Based on our previous study, the interaction of the M12 protein with HSA is speculated to influence the...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-10-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
| Subjects: | |
| Online Access: | https://journals.lww.com/japtr/fulltext/2024/15040/human_serum_albumin_regulates_the_ornithine.12.aspx |
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| Summary: | The M12 protein is the main virulence factor of Streptococcus pyogenes as a mediator in the bacteria’s adhesion to numerous human proteins, including human serum albumin (HSA) and immunoglobulin. Based on our previous study, the interaction of the M12 protein with HSA is speculated to influence the synthesis of the ornithine carbamoyltransferase 12 (OCTase12), which may attach human fibronectin, preventing the bacteria from being recognized as a foreign material, thus defending itself against the human immune system. The purpose of this study was to use an immunological method to examine the impact of HSA on OCTase12 synthesis. The methods of this study were conducted with the overproduction of OCTase 12 in Escherichia coli BL21(DE3) plysS containing pEToctase12 gene fused with polyhistidine tag to purify the protein using affinity chromatography with nickel as the ligand. The purified protein was then characterized using dot blot and the influence of HSA was investigated using Western blot analyses. This investigation was conducted by cultivating the E. coli in conditions with and without HSA. The Western blot analysis using the antibody against total cell proteins of S. pyogenes CS24 indicated that OCTase12 production was increased by the presence of HSA. Therefore, this early finding showing that HSA may influence the expression of the octase 12 gene. |
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| ISSN: | 0976-2094 |