Determination of lactose in milk by high performance liquid chromatography-evaporative light scattering detector with RCM-monosaccharide Ca2+ column
<b>Objective:</b> To achieve rapid detection of lactose content in milk, a new method for the determination of lactose in milk based on RCM-monosaccharide Ca<sup>2+</sup> column and high-performance liquid chromatography has been established. <b>Methods:</b> Milk...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Editorial Office of Food and Machinery
2023-12-01
|
| Series: | Shipin yu jixie |
| Subjects: | |
| Online Access: | http://www.ifoodmm.com/spyjxen/article/abstract/20231110 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <b>Objective:</b> To achieve rapid detection of lactose content in milk, a new method for the determination of lactose in milk based on RCM-monosaccharide Ca<sup>2+</sup> column and high-performance liquid chromatography has been established. <b>Methods:</b> Milk was precipitated with protein in a 0.2 g/mL trichloroacetic acid solution, and the resulting filtrate was diluted 100 times and filtered with a membrane before entering a high-performance liquid chromatography system. It was separated by an RCM-monosaccharide Ca<sup>2+</sup> column and detected by an evaporative light scattering detector. <b>Results:</b> The results showed that lactose had a good correlation between peak area and concentration in the linear range （20~100 mg/L）, with an <i>R</i><sup>2</sup> of 0.999 8. When the lactose spiking levels were 15, 40, and 80 mg/g, the recovery range was between 90.96% and 98.23%, the detection limit was 3.6 μg/g, and the quantitative limit was 12 μg/g, the lactose concentration was determined within 6 minutes. 11 commercial milk samples were measured by this method, and the results were consistent with the national standard method. <b>Conclusion:</b> The precision, repeatability, and recovery rate of this method met relevant regulations, and the detection results were accurate and time-consuming. It is suitable for rapid detection of lactose content in milk. |
|---|---|
| ISSN: | 1003-5788 |