Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice
The inflammatory and autoimmune events preceding clinical symptoms in rheumatoid arthritis (RA) and other autoimmune diseases are difficult to study in human patients. Therefore, animal models that share immunologic and clinical features with human RA, such as pristane-induced arthritis (PIA), are v...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/2641098 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850227804039282688 |
|---|---|
| author | Cristiano Rossato Layra Lucy Albuquerque Iana Suly Santos Katz Andrea Borrego Wafa Hanna Koury Cabrera Mônica Spadafora-Ferreira Orlando Garcia Ribeiro Nancy Starobinas Olga Martinez Ibañez Marcelo De Franco José Ricardo Jensen |
| author_facet | Cristiano Rossato Layra Lucy Albuquerque Iana Suly Santos Katz Andrea Borrego Wafa Hanna Koury Cabrera Mônica Spadafora-Ferreira Orlando Garcia Ribeiro Nancy Starobinas Olga Martinez Ibañez Marcelo De Franco José Ricardo Jensen |
| author_sort | Cristiano Rossato |
| collection | DOAJ |
| description | The inflammatory and autoimmune events preceding clinical symptoms in rheumatoid arthritis (RA) and other autoimmune diseases are difficult to study in human patients. Therefore, animal models that share immunologic and clinical features with human RA, such as pristane-induced arthritis (PIA), are valuable tools for assessing the primordial events related to arthritis susceptibility. PIA-resistant HIII and susceptible LIII mice were injected i.p. with pristane, and peritoneal lavage fluid was harvested in the early (7 days) and late (35 days) preclinical phases of PIA. Chemokine and cytokine levels were measured in lavage supernatant with ELISA, peritoneal inflammatory leukocytes were immunophenotyped by flow cytometry, and gene expression was determined by qRT-PCR. Leukocyte recruitment was quantitatively and qualitatively divergent in the peritoneum of HIII and LIII mice, with an early increase of CC chemokines (CCL2/CCL3/CCL5/CCL12/CCL22) in the susceptible LIII strain. Also, cytokines such as IL-12p40, IL-23, and IL-18 were elevated in LIII mice while IL-6 was increased in HIII animals. The results show that an early peritoneal CC chemokine response is an important feature of arthritis susceptibility and defines potential biomarkers in this model. |
| format | Article |
| id | doaj-art-24250f49b2bf471c8e061da076af454e |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-24250f49b2bf471c8e061da076af454e2025-08-20T02:04:43ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/26410982641098Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in MiceCristiano Rossato0Layra Lucy Albuquerque1Iana Suly Santos Katz2Andrea Borrego3Wafa Hanna Koury Cabrera4Mônica Spadafora-Ferreira5Orlando Garcia Ribeiro6Nancy Starobinas7Olga Martinez Ibañez8Marcelo De Franco9José Ricardo Jensen10Immunogenetics Laboratory, Butantan Institute, Avenida Vital Brasil, 1500, São Paulo 05503-900, BrazilUnião Educacional do Norte, Rio Branco 69915-901, BrazilDiagnostics Section, Pasteur Institute, Avenida Paulista, 393, São Paulo 01311-000, BrazilImmunogenetics Laboratory, Butantan Institute, Avenida Vital Brasil, 1500, São Paulo 05503-900, BrazilImmunogenetics Laboratory, Butantan Institute, Avenida Vital Brasil, 1500, São Paulo 05503-900, BrazilImmunogenetics Laboratory, Butantan Institute, Avenida Vital Brasil, 1500, São Paulo 05503-900, BrazilImmunogenetics Laboratory, Butantan Institute, Avenida Vital Brasil, 1500, São Paulo 05503-900, BrazilImmunogenetics Laboratory, Butantan Institute, Avenida Vital Brasil, 1500, São Paulo 05503-900, BrazilImmunogenetics Laboratory, Butantan Institute, Avenida Vital Brasil, 1500, São Paulo 05503-900, BrazilImmunogenetics Laboratory, Butantan Institute, Avenida Vital Brasil, 1500, São Paulo 05503-900, BrazilImmunogenetics Laboratory, Butantan Institute, Avenida Vital Brasil, 1500, São Paulo 05503-900, BrazilThe inflammatory and autoimmune events preceding clinical symptoms in rheumatoid arthritis (RA) and other autoimmune diseases are difficult to study in human patients. Therefore, animal models that share immunologic and clinical features with human RA, such as pristane-induced arthritis (PIA), are valuable tools for assessing the primordial events related to arthritis susceptibility. PIA-resistant HIII and susceptible LIII mice were injected i.p. with pristane, and peritoneal lavage fluid was harvested in the early (7 days) and late (35 days) preclinical phases of PIA. Chemokine and cytokine levels were measured in lavage supernatant with ELISA, peritoneal inflammatory leukocytes were immunophenotyped by flow cytometry, and gene expression was determined by qRT-PCR. Leukocyte recruitment was quantitatively and qualitatively divergent in the peritoneum of HIII and LIII mice, with an early increase of CC chemokines (CCL2/CCL3/CCL5/CCL12/CCL22) in the susceptible LIII strain. Also, cytokines such as IL-12p40, IL-23, and IL-18 were elevated in LIII mice while IL-6 was increased in HIII animals. The results show that an early peritoneal CC chemokine response is an important feature of arthritis susceptibility and defines potential biomarkers in this model.http://dx.doi.org/10.1155/2019/2641098 |
| spellingShingle | Cristiano Rossato Layra Lucy Albuquerque Iana Suly Santos Katz Andrea Borrego Wafa Hanna Koury Cabrera Mônica Spadafora-Ferreira Orlando Garcia Ribeiro Nancy Starobinas Olga Martinez Ibañez Marcelo De Franco José Ricardo Jensen Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice Journal of Immunology Research |
| title | Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice |
| title_full | Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice |
| title_fullStr | Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice |
| title_full_unstemmed | Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice |
| title_short | Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice |
| title_sort | early peritoneal cc chemokine production correlates with divergent inflammatory phenotypes and susceptibility to experimental arthritis in mice |
| url | http://dx.doi.org/10.1155/2019/2641098 |
| work_keys_str_mv | AT cristianorossato earlyperitonealccchemokineproductioncorrelateswithdivergentinflammatoryphenotypesandsusceptibilitytoexperimentalarthritisinmice AT layralucyalbuquerque earlyperitonealccchemokineproductioncorrelateswithdivergentinflammatoryphenotypesandsusceptibilitytoexperimentalarthritisinmice AT ianasulysantoskatz earlyperitonealccchemokineproductioncorrelateswithdivergentinflammatoryphenotypesandsusceptibilitytoexperimentalarthritisinmice AT andreaborrego earlyperitonealccchemokineproductioncorrelateswithdivergentinflammatoryphenotypesandsusceptibilitytoexperimentalarthritisinmice AT wafahannakourycabrera earlyperitonealccchemokineproductioncorrelateswithdivergentinflammatoryphenotypesandsusceptibilitytoexperimentalarthritisinmice AT monicaspadaforaferreira earlyperitonealccchemokineproductioncorrelateswithdivergentinflammatoryphenotypesandsusceptibilitytoexperimentalarthritisinmice AT orlandogarciaribeiro earlyperitonealccchemokineproductioncorrelateswithdivergentinflammatoryphenotypesandsusceptibilitytoexperimentalarthritisinmice AT nancystarobinas earlyperitonealccchemokineproductioncorrelateswithdivergentinflammatoryphenotypesandsusceptibilitytoexperimentalarthritisinmice AT olgamartinezibanez earlyperitonealccchemokineproductioncorrelateswithdivergentinflammatoryphenotypesandsusceptibilitytoexperimentalarthritisinmice AT marcelodefranco earlyperitonealccchemokineproductioncorrelateswithdivergentinflammatoryphenotypesandsusceptibilitytoexperimentalarthritisinmice AT josericardojensen earlyperitonealccchemokineproductioncorrelateswithdivergentinflammatoryphenotypesandsusceptibilitytoexperimentalarthritisinmice |