Comprehensive physiological, transcriptomic, and metabolomic analyses revealed the regulation mechanism of evergreen and cold resistance of Pinus koraiensis needles
Abstract As a significant fruit and timber tree species among conifers, Pinus koraiensis remains it evergreen status throughout the harsh winters of the north, a testament to its intricate and prolonged evolutionary adaptation. This study delves into the annual trends of physiological indicators, ge...
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2024-12-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-05924-6 |
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| author | Yan Li Xin Li Dan Peng Jiaxin Luo Shuai Zhu Haibo Du Xiaoning Li Jiafeng Zhang Jun Meng Xiaona Pei Xiyang Zhao |
| author_facet | Yan Li Xin Li Dan Peng Jiaxin Luo Shuai Zhu Haibo Du Xiaoning Li Jiafeng Zhang Jun Meng Xiaona Pei Xiyang Zhao |
| author_sort | Yan Li |
| collection | DOAJ |
| description | Abstract As a significant fruit and timber tree species among conifers, Pinus koraiensis remains it evergreen status throughout the harsh winters of the north, a testament to its intricate and prolonged evolutionary adaptation. This study delves into the annual trends of physiological indicators, gene expression levels, and metabolite accumulation to dissect the seasonal adaptability of P. koraiensis needles. Chlorophyll content reaches its zenith primarily between July and September, whereas carotenoids persist until spring. Additionally, notable seasonal variations are observed in the levels of soluble sugar and protein. Transcriptome data is categorized into four distinct stages: spring (S2), summer (S3-S4), autumn (S5), and winter (S6-S1). The differential expression of transcription factor genes, including bHLH, MYB-related, AP2/ERF, C3H, and NAC, provides insights into the needles’ seasonal adaptations. Analysis of chlorophyll and carotenoid metabolism, sugar metabolism, and the MAPK signaling pathway identifies PSY5 (Cluster-50735.3), AMY13 (Cluster-37114.0), pgm1 (Cluster-46022.0), and MEKK1-1 (Cluster-33069.0) may as potential key genes involved in sustaining the needle’s evergreen nature and cold resistance. Ultimately, a comprehensive annual adaptability map for P. koraiensis is proposed, enhancing understanding of its responses to seasonal variations. |
| format | Article |
| id | doaj-art-2423d5364e1e4318adaa8ec9c8790587 |
| institution | OA Journals |
| issn | 1471-2229 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj-art-2423d5364e1e4318adaa8ec9c87905872025-08-20T02:31:54ZengBMCBMC Plant Biology1471-22292024-12-0124111710.1186/s12870-024-05924-6Comprehensive physiological, transcriptomic, and metabolomic analyses revealed the regulation mechanism of evergreen and cold resistance of Pinus koraiensis needlesYan Li0Xin Li1Dan Peng2Jiaxin Luo3Shuai Zhu4Haibo Du5Xiaoning Li6Jiafeng Zhang7Jun Meng8Xiaona Pei9Xiyang Zhao10Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural UniversityJilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural UniversityJilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural UniversityJilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural UniversityJilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural UniversityBaicheng Forestry Science Research InstituteBaicheng Forestry Science Research InstituteYongji County Forest Seed StationJilin Forest Seedling Management StationCollege of Horticulture, Jilin Agricultural UniversityJilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural UniversityAbstract As a significant fruit and timber tree species among conifers, Pinus koraiensis remains it evergreen status throughout the harsh winters of the north, a testament to its intricate and prolonged evolutionary adaptation. This study delves into the annual trends of physiological indicators, gene expression levels, and metabolite accumulation to dissect the seasonal adaptability of P. koraiensis needles. Chlorophyll content reaches its zenith primarily between July and September, whereas carotenoids persist until spring. Additionally, notable seasonal variations are observed in the levels of soluble sugar and protein. Transcriptome data is categorized into four distinct stages: spring (S2), summer (S3-S4), autumn (S5), and winter (S6-S1). The differential expression of transcription factor genes, including bHLH, MYB-related, AP2/ERF, C3H, and NAC, provides insights into the needles’ seasonal adaptations. Analysis of chlorophyll and carotenoid metabolism, sugar metabolism, and the MAPK signaling pathway identifies PSY5 (Cluster-50735.3), AMY13 (Cluster-37114.0), pgm1 (Cluster-46022.0), and MEKK1-1 (Cluster-33069.0) may as potential key genes involved in sustaining the needle’s evergreen nature and cold resistance. Ultimately, a comprehensive annual adaptability map for P. koraiensis is proposed, enhancing understanding of its responses to seasonal variations.https://doi.org/10.1186/s12870-024-05924-6Pinus koraiensis needlesSeasonal adaptationPhysiologyTranscriptomeMetabolome |
| spellingShingle | Yan Li Xin Li Dan Peng Jiaxin Luo Shuai Zhu Haibo Du Xiaoning Li Jiafeng Zhang Jun Meng Xiaona Pei Xiyang Zhao Comprehensive physiological, transcriptomic, and metabolomic analyses revealed the regulation mechanism of evergreen and cold resistance of Pinus koraiensis needles BMC Plant Biology Pinus koraiensis needles Seasonal adaptation Physiology Transcriptome Metabolome |
| title | Comprehensive physiological, transcriptomic, and metabolomic analyses revealed the regulation mechanism of evergreen and cold resistance of Pinus koraiensis needles |
| title_full | Comprehensive physiological, transcriptomic, and metabolomic analyses revealed the regulation mechanism of evergreen and cold resistance of Pinus koraiensis needles |
| title_fullStr | Comprehensive physiological, transcriptomic, and metabolomic analyses revealed the regulation mechanism of evergreen and cold resistance of Pinus koraiensis needles |
| title_full_unstemmed | Comprehensive physiological, transcriptomic, and metabolomic analyses revealed the regulation mechanism of evergreen and cold resistance of Pinus koraiensis needles |
| title_short | Comprehensive physiological, transcriptomic, and metabolomic analyses revealed the regulation mechanism of evergreen and cold resistance of Pinus koraiensis needles |
| title_sort | comprehensive physiological transcriptomic and metabolomic analyses revealed the regulation mechanism of evergreen and cold resistance of pinus koraiensis needles |
| topic | Pinus koraiensis needles Seasonal adaptation Physiology Transcriptome Metabolome |
| url | https://doi.org/10.1186/s12870-024-05924-6 |
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