Acceleration of Functional Maturation and Differentiation of Neonatal Porcine Islet Cell Monolayers Shortly In Vitro Cocultured with Microencapsulated Sertoli Cells
The limited availability of cadaveric human donor pancreata as well as the incomplete success of the Edmonton protocol for human islet allografts fasten search for new sources of insulin the producing cells for substitution cell therapy of insulin-dependent diabetes mellitus (T1DM). Starting from is...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.4061/2010/587213 |
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| author | Francesca Mancuso Mario Calvitti Giovanni Luca Claudio Nastruzzi Tiziano Baroni Stefania Mazzitelli Ennio Becchetti Iva Arato Carlo Boselli Monique D. Ngo Nselel Riccardo Calafiore |
| author_facet | Francesca Mancuso Mario Calvitti Giovanni Luca Claudio Nastruzzi Tiziano Baroni Stefania Mazzitelli Ennio Becchetti Iva Arato Carlo Boselli Monique D. Ngo Nselel Riccardo Calafiore |
| author_sort | Francesca Mancuso |
| collection | DOAJ |
| description | The limited availability of cadaveric human donor pancreata as well as the incomplete success of the Edmonton protocol for human islet allografts fasten search for new sources of insulin the producing cells for substitution cell therapy of insulin-dependent diabetes mellitus (T1DM). Starting from isolated neonatal porcine pancreatic islets (NPIs), we have obtained cell monolayers that were exposed to microencapsulated monolayered Sertoli cells (ESCs) for different time periods (7, 14, 21 days). To assess the development of the cocultured cell monolayers, we have studied either endocrine cell phenotype differentiation markers or c-kit, a hematopoietic stem cell marker, has recently been involved with growth and differentiation of β-cell subpopulations in human as well as rodent animal models. ESC which were found to either accelerate maturation and differentiation of the NPIs β-cell phenotype or identify an islet cell subpopulation that was marked positively for c-kit. The insulin/c-kit positive cells might represent a new, still unknown functionally immature β-cell like element in the porcine pancreas. Acceleration of maturation and differentiation of our NPI cell monolayers might generate a potential new opportunity to develop insulin-producing cells that may suite experimental trials for cell therapy of T1DM. |
| format | Article |
| id | doaj-art-24223df4bb2e4f10b37b9a20ca11ce29 |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-24223df4bb2e4f10b37b9a20ca11ce292025-08-20T03:54:52ZengWileyStem Cells International1687-96782010-01-01201010.4061/2010/587213587213Acceleration of Functional Maturation and Differentiation of Neonatal Porcine Islet Cell Monolayers Shortly In Vitro Cocultured with Microencapsulated Sertoli CellsFrancesca Mancuso0Mario Calvitti1Giovanni Luca2Claudio Nastruzzi3Tiziano Baroni4Stefania Mazzitelli5Ennio Becchetti6Iva Arato7Carlo Boselli8Monique D. Ngo Nselel9Riccardo Calafiore10Section of Internal Medicine and Endocrine and Metabolic Sciences, Department of Internal Medicine, University of Perugia, 06126 Perugia, ItalyDepartment of Experimental Medicine and Biochemical Sciences, University of Perugia, 06126 Perugia, ItalySection of Internal Medicine and Endocrine and Metabolic Sciences, Department of Internal Medicine, University of Perugia, 06126 Perugia, ItalyDepartment of Chemistry and Technology of the Drug, School of Pharmacy, University of Perugia, 06126 Perugia, ItalyDepartment of Experimental Medicine and Biochemical Sciences, University of Perugia, 06126 Perugia, ItalyDepartment of Chemistry and Technology of the Drug, School of Pharmacy, University of Perugia, 06126 Perugia, ItalyDepartment of Experimental Medicine and Biochemical Sciences, University of Perugia, 06126 Perugia, ItalySection of Internal Medicine and Endocrine and Metabolic Sciences, Department of Internal Medicine, University of Perugia, 06126 Perugia, ItalyDepartment of Surgery, University of Perugia, 06126 Perugia, ItalySection of Internal Medicine and Endocrine and Metabolic Sciences, Department of Internal Medicine, University of Perugia, 06126 Perugia, ItalySection of Internal Medicine and Endocrine and Metabolic Sciences, Department of Internal Medicine, University of Perugia, 06126 Perugia, ItalyThe limited availability of cadaveric human donor pancreata as well as the incomplete success of the Edmonton protocol for human islet allografts fasten search for new sources of insulin the producing cells for substitution cell therapy of insulin-dependent diabetes mellitus (T1DM). Starting from isolated neonatal porcine pancreatic islets (NPIs), we have obtained cell monolayers that were exposed to microencapsulated monolayered Sertoli cells (ESCs) for different time periods (7, 14, 21 days). To assess the development of the cocultured cell monolayers, we have studied either endocrine cell phenotype differentiation markers or c-kit, a hematopoietic stem cell marker, has recently been involved with growth and differentiation of β-cell subpopulations in human as well as rodent animal models. ESC which were found to either accelerate maturation and differentiation of the NPIs β-cell phenotype or identify an islet cell subpopulation that was marked positively for c-kit. The insulin/c-kit positive cells might represent a new, still unknown functionally immature β-cell like element in the porcine pancreas. Acceleration of maturation and differentiation of our NPI cell monolayers might generate a potential new opportunity to develop insulin-producing cells that may suite experimental trials for cell therapy of T1DM.http://dx.doi.org/10.4061/2010/587213 |
| spellingShingle | Francesca Mancuso Mario Calvitti Giovanni Luca Claudio Nastruzzi Tiziano Baroni Stefania Mazzitelli Ennio Becchetti Iva Arato Carlo Boselli Monique D. Ngo Nselel Riccardo Calafiore Acceleration of Functional Maturation and Differentiation of Neonatal Porcine Islet Cell Monolayers Shortly In Vitro Cocultured with Microencapsulated Sertoli Cells Stem Cells International |
| title | Acceleration of Functional Maturation and Differentiation of Neonatal Porcine Islet Cell Monolayers Shortly In Vitro Cocultured with Microencapsulated Sertoli Cells |
| title_full | Acceleration of Functional Maturation and Differentiation of Neonatal Porcine Islet Cell Monolayers Shortly In Vitro Cocultured with Microencapsulated Sertoli Cells |
| title_fullStr | Acceleration of Functional Maturation and Differentiation of Neonatal Porcine Islet Cell Monolayers Shortly In Vitro Cocultured with Microencapsulated Sertoli Cells |
| title_full_unstemmed | Acceleration of Functional Maturation and Differentiation of Neonatal Porcine Islet Cell Monolayers Shortly In Vitro Cocultured with Microencapsulated Sertoli Cells |
| title_short | Acceleration of Functional Maturation and Differentiation of Neonatal Porcine Islet Cell Monolayers Shortly In Vitro Cocultured with Microencapsulated Sertoli Cells |
| title_sort | acceleration of functional maturation and differentiation of neonatal porcine islet cell monolayers shortly in vitro cocultured with microencapsulated sertoli cells |
| url | http://dx.doi.org/10.4061/2010/587213 |
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