Extraction and purification of L-Asparaginase II from local isolate of Proteus vulgaris
Forty one isolates of genus Proteus were collected from 140 clinical specimens such as urine, stool, wound, burn, and ear swabs from patients of both sex. These isolates were identified to three Proteus spp. P. mirabilis, P. vulgaris and P. penneri .The ability of these bacteria to produce L-aspa...
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University of Baghdad, College of Science for Women
2011-03-01
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| Series: | مجلة بغداد للعلوم |
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| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2524 |
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| description | Forty one isolates of genus Proteus were collected from 140 clinical specimens such as urine, stool, wound, burn, and ear swabs from patients of both sex. These isolates were identified to three Proteus spp. P. mirabilis, P. vulgaris and P. penneri .The ability of these bacteria to produce L-asparaginase II by using semi quantitative and quantitative methods was determined. P. vulgaris Pv.U.92 was distinguished for high level of L-asparaginase II production with specific activity 1.97 U/mg. Optimum conditions for enzyme production were determined; D medium with 0.3% of L-asparagine at pH 7.5 with temperature degree 35°C for incubation.
Ultrasonication was used to destroy the P. vulgaris Pv.U.92 cells then ASNase II was extracted and purified throughout several purification steps including precipitation with (NH4)2SO4(60-80%), DEAE-cellulose ion exchanger chromatography followed by Sephacryl S-300 filtration. The specific activity was 155.6 U/ mg and the purification fold was 27.3 with 10.4% yield. |
| format | Article |
| id | doaj-art-2410b8ad81a54aeaa96be6d6523ee33f |
| institution | Kabale University |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2011-03-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-2410b8ad81a54aeaa96be6d6523ee33f2025-08-20T03:33:14ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862011-03-018110.21123/bsj.8.1.509-518Extraction and purification of L-Asparaginase II from local isolate of Proteus vulgarisBaghdad Science JournalForty one isolates of genus Proteus were collected from 140 clinical specimens such as urine, stool, wound, burn, and ear swabs from patients of both sex. These isolates were identified to three Proteus spp. P. mirabilis, P. vulgaris and P. penneri .The ability of these bacteria to produce L-asparaginase II by using semi quantitative and quantitative methods was determined. P. vulgaris Pv.U.92 was distinguished for high level of L-asparaginase II production with specific activity 1.97 U/mg. Optimum conditions for enzyme production were determined; D medium with 0.3% of L-asparagine at pH 7.5 with temperature degree 35°C for incubation. Ultrasonication was used to destroy the P. vulgaris Pv.U.92 cells then ASNase II was extracted and purified throughout several purification steps including precipitation with (NH4)2SO4(60-80%), DEAE-cellulose ion exchanger chromatography followed by Sephacryl S-300 filtration. The specific activity was 155.6 U/ mg and the purification fold was 27.3 with 10.4% yield.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2524L-Asparaginase II, P. vulgaris, DEAE-cellulose, Sephacryl S-300. |
| spellingShingle | Baghdad Science Journal Extraction and purification of L-Asparaginase II from local isolate of Proteus vulgaris مجلة بغداد للعلوم L-Asparaginase II, P. vulgaris, DEAE-cellulose, Sephacryl S-300. |
| title | Extraction and purification of L-Asparaginase II from local isolate of Proteus vulgaris |
| title_full | Extraction and purification of L-Asparaginase II from local isolate of Proteus vulgaris |
| title_fullStr | Extraction and purification of L-Asparaginase II from local isolate of Proteus vulgaris |
| title_full_unstemmed | Extraction and purification of L-Asparaginase II from local isolate of Proteus vulgaris |
| title_short | Extraction and purification of L-Asparaginase II from local isolate of Proteus vulgaris |
| title_sort | extraction and purification of l asparaginase ii from local isolate of proteus vulgaris |
| topic | L-Asparaginase II, P. vulgaris, DEAE-cellulose, Sephacryl S-300. |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2524 |
| work_keys_str_mv | AT baghdadsciencejournal extractionandpurificationoflasparaginaseiifromlocalisolateofproteusvulgaris |