Isolation, Identification, and Antimicrobial Study of Yersinia spp. Isolated from Traditional and Industrial Olivier Salad Specimens in Tehran
Background: Yersinia enterocolitica is a gram-negative bacteria and one of the most important food-borne bacteria that causes yersiniosis. Several studies have investigated various species of this gastrointestinal pathogen in food and water sources. Although Olivier salad is a ready-to-eat and easil...
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Shahid Sadoughi University of Medical Sciences and Health Services
2025-02-01
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| Series: | Journal of Nutrition and Food Security |
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| Online Access: | http://jnfs.ssu.ac.ir/article-1-1051-en.pdf |
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| author | Farnaz Eskandarzadeh Mohammad Reza Mohammadi Abbas Rahimi Foroushani Mohammad Mehdi Soltan Dallal |
| author_facet | Farnaz Eskandarzadeh Mohammad Reza Mohammadi Abbas Rahimi Foroushani Mohammad Mehdi Soltan Dallal |
| author_sort | Farnaz Eskandarzadeh |
| collection | DOAJ |
| description | Background: Yersinia enterocolitica is a gram-negative bacteria and one of the most important food-borne bacteria that causes yersiniosis. Several studies have investigated various species of this gastrointestinal pathogen in food and water sources. Although Olivier salad is a ready-to-eat and easily available cold food, it is highly susceptible to microbial contaminations. This study aims to investigate the presence of Yersinia spp., especially the pathogenic Yersinia enterocolitica in Olivier salad. Methods: In this cross-sectional descriptive study, 150 specimens (traditional and industrial Olivier salad) were collected and tested for the presence of Yersinia spp. Using the Iranian National Standard No. 4556. Obtained data were analyzed statistically using descriptive statistics in IBM SPSS. Results: Yersinia spp. contamination was found in only 6% of traditional specimens (6 out of 100). Based on biochemical reactions, four isolates of Yersinia enterocolitica and two isolates of Yersinia intermedia were identified from six Yersinia isolates. The biotype of Yersinia enterocolitica was investigated, and three strains of biotype 1A and one strain of biotype 1B were identified. The human pathogenic serotype was not found in the serotype analysis. Conclusion: The study results showed that specimens of traditionally prepared Olivier salad were contaminated with Yersinia spp. Yersinia enterocolitica is the most prevalent species as expected, and the analysis of the strains of this species revealed that it also contains other biotypes, including the highly pathogenic biotype 1B. |
| format | Article |
| id | doaj-art-2405f9badf9243fdb1cc6b41b1eea3a9 |
| institution | Kabale University |
| issn | 2476-7417 2476-7425 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Shahid Sadoughi University of Medical Sciences and Health Services |
| record_format | Article |
| series | Journal of Nutrition and Food Security |
| spelling | doaj-art-2405f9badf9243fdb1cc6b41b1eea3a92025-02-08T10:57:36ZengShahid Sadoughi University of Medical Sciences and Health ServicesJournal of Nutrition and Food Security2476-74172476-74252025-02-011013241Isolation, Identification, and Antimicrobial Study of Yersinia spp. Isolated from Traditional and Industrial Olivier Salad Specimens in TehranFarnaz Eskandarzadeh0Mohammad Reza Mohammadi1Abbas Rahimi Foroushani2Mohammad Mehdi Soltan Dallal3 Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Food Microbiology Research Centre, Tehran University of Medical Sciences, Tehran, Iran Background: Yersinia enterocolitica is a gram-negative bacteria and one of the most important food-borne bacteria that causes yersiniosis. Several studies have investigated various species of this gastrointestinal pathogen in food and water sources. Although Olivier salad is a ready-to-eat and easily available cold food, it is highly susceptible to microbial contaminations. This study aims to investigate the presence of Yersinia spp., especially the pathogenic Yersinia enterocolitica in Olivier salad. Methods: In this cross-sectional descriptive study, 150 specimens (traditional and industrial Olivier salad) were collected and tested for the presence of Yersinia spp. Using the Iranian National Standard No. 4556. Obtained data were analyzed statistically using descriptive statistics in IBM SPSS. Results: Yersinia spp. contamination was found in only 6% of traditional specimens (6 out of 100). Based on biochemical reactions, four isolates of Yersinia enterocolitica and two isolates of Yersinia intermedia were identified from six Yersinia isolates. The biotype of Yersinia enterocolitica was investigated, and three strains of biotype 1A and one strain of biotype 1B were identified. The human pathogenic serotype was not found in the serotype analysis. Conclusion: The study results showed that specimens of traditionally prepared Olivier salad were contaminated with Yersinia spp. Yersinia enterocolitica is the most prevalent species as expected, and the analysis of the strains of this species revealed that it also contains other biotypes, including the highly pathogenic biotype 1B.http://jnfs.ssu.ac.ir/article-1-1051-en.pdfolivier saladfoodborne diseasebacterial contaminationyersinia enterocolitica |
| spellingShingle | Farnaz Eskandarzadeh Mohammad Reza Mohammadi Abbas Rahimi Foroushani Mohammad Mehdi Soltan Dallal Isolation, Identification, and Antimicrobial Study of Yersinia spp. Isolated from Traditional and Industrial Olivier Salad Specimens in Tehran Journal of Nutrition and Food Security olivier salad foodborne disease bacterial contamination yersinia enterocolitica |
| title | Isolation, Identification, and Antimicrobial Study of Yersinia spp. Isolated from Traditional and Industrial Olivier Salad Specimens in Tehran |
| title_full | Isolation, Identification, and Antimicrobial Study of Yersinia spp. Isolated from Traditional and Industrial Olivier Salad Specimens in Tehran |
| title_fullStr | Isolation, Identification, and Antimicrobial Study of Yersinia spp. Isolated from Traditional and Industrial Olivier Salad Specimens in Tehran |
| title_full_unstemmed | Isolation, Identification, and Antimicrobial Study of Yersinia spp. Isolated from Traditional and Industrial Olivier Salad Specimens in Tehran |
| title_short | Isolation, Identification, and Antimicrobial Study of Yersinia spp. Isolated from Traditional and Industrial Olivier Salad Specimens in Tehran |
| title_sort | isolation identification and antimicrobial study of yersinia spp isolated from traditional and industrial olivier salad specimens in tehran |
| topic | olivier salad foodborne disease bacterial contamination yersinia enterocolitica |
| url | http://jnfs.ssu.ac.ir/article-1-1051-en.pdf |
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