Effect Of Methanolic Leaves Extract of Telfairia occidentalis on 2,4-Dinitrophenylhydrazine Induced Anaemic Rats

Anaemia constitutes a serious health problem in many tropical countries including Nigeria because of the high prevalence of malaria and other parasitic infections which possibly leads to a decrease in hemoglobin. Fluted pumpkin has been reported to be very good in building the constituents of the b...

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Main Authors: Suleiman, Z.A., Salahuddeen, Y., Sabiu, U.A.
Format: Article
Language:English
Published: Umaru Musa Yar'adua University, Katsina, Nigeria 2022-06-01
Series:UMYU Journal of Microbiology Research
Subjects:
Online Access:https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/34
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author Suleiman, Z.A.
Salahuddeen, Y.
Sabiu, U.A.
author_facet Suleiman, Z.A.
Salahuddeen, Y.
Sabiu, U.A.
author_sort Suleiman, Z.A.
collection DOAJ
description Anaemia constitutes a serious health problem in many tropical countries including Nigeria because of the high prevalence of malaria and other parasitic infections which possibly leads to a decrease in hemoglobin. Fluted pumpkin has been reported to be very good in building the constituents of the blood and also replacing them. This study investigated the effects of the methanolic leaves extract of fluted pumpkin on some hematological parameters in 2, 4-dinitrophenylhydrazine-induced anaemic rats. Thirty Wister rats of both sexes were separated into five groups of six rats each. Group 1 serves as normal control, while groups 2-5 were induced with anaemia using 40mg/kg body weight of 2,4- dinitrophenylhydrazine. Group 2 serves as the negative control without treatment, group 3 were given 20 mg/kg (b.w) of standard hematinic drug astymin, and group 4 and 5 were administered with 200 and 400 mg/kg w/w methanolic leaves extract of Telfairia occidentals respectively. All rats were fed with grower’s mash and water ad libitum for 21 days. The rats were sacrificed and blood samples were collected for hematological analysis. All analysis was done using standard methods. The result of this study demonstrated that oral administration of 200 mg/kg and 400mg/kg of the extract increased the haematological parameters under investigation. A significant (p<0.05) increase in haematinic activity was observed in group 3 and 5,   and it was concluded that methanolic leaves extract of fluted pumpkin could be effective in ameliorating anaemia.
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publishDate 2022-06-01
publisher Umaru Musa Yar'adua University, Katsina, Nigeria
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spelling doaj-art-23fc5996282946da805b0bdaecc03ede2025-08-20T02:06:44ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222022-06-017110.47430/ujmr.2271.01134Effect Of Methanolic Leaves Extract of Telfairia occidentalis on 2,4-Dinitrophenylhydrazine Induced Anaemic RatsSuleiman, Z.A.0Salahuddeen, Y.1Sabiu, U.A.2Department of Biochemistry, Umaru Musa Yar’adua University, Katsina, Nigeria.Department of Biochemistry, Umaru Musa Yar’adua University, Katsina, Nigeria.Department of Biochemistry and Molecular Biology, Federal University Dutsin-Ma, Katsina. Anaemia constitutes a serious health problem in many tropical countries including Nigeria because of the high prevalence of malaria and other parasitic infections which possibly leads to a decrease in hemoglobin. Fluted pumpkin has been reported to be very good in building the constituents of the blood and also replacing them. This study investigated the effects of the methanolic leaves extract of fluted pumpkin on some hematological parameters in 2, 4-dinitrophenylhydrazine-induced anaemic rats. Thirty Wister rats of both sexes were separated into five groups of six rats each. Group 1 serves as normal control, while groups 2-5 were induced with anaemia using 40mg/kg body weight of 2,4- dinitrophenylhydrazine. Group 2 serves as the negative control without treatment, group 3 were given 20 mg/kg (b.w) of standard hematinic drug astymin, and group 4 and 5 were administered with 200 and 400 mg/kg w/w methanolic leaves extract of Telfairia occidentals respectively. All rats were fed with grower’s mash and water ad libitum for 21 days. The rats were sacrificed and blood samples were collected for hematological analysis. All analysis was done using standard methods. The result of this study demonstrated that oral administration of 200 mg/kg and 400mg/kg of the extract increased the haematological parameters under investigation. A significant (p<0.05) increase in haematinic activity was observed in group 3 and 5,   and it was concluded that methanolic leaves extract of fluted pumpkin could be effective in ameliorating anaemia. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/34AnemiaTelfairia occidentalis2, 4-dinitrophenylhydrazineHaematological parameters
spellingShingle Suleiman, Z.A.
Salahuddeen, Y.
Sabiu, U.A.
Effect Of Methanolic Leaves Extract of Telfairia occidentalis on 2,4-Dinitrophenylhydrazine Induced Anaemic Rats
UMYU Journal of Microbiology Research
Anemia
Telfairia occidentalis
2, 4-dinitrophenylhydrazine
Haematological parameters
title Effect Of Methanolic Leaves Extract of Telfairia occidentalis on 2,4-Dinitrophenylhydrazine Induced Anaemic Rats
title_full Effect Of Methanolic Leaves Extract of Telfairia occidentalis on 2,4-Dinitrophenylhydrazine Induced Anaemic Rats
title_fullStr Effect Of Methanolic Leaves Extract of Telfairia occidentalis on 2,4-Dinitrophenylhydrazine Induced Anaemic Rats
title_full_unstemmed Effect Of Methanolic Leaves Extract of Telfairia occidentalis on 2,4-Dinitrophenylhydrazine Induced Anaemic Rats
title_short Effect Of Methanolic Leaves Extract of Telfairia occidentalis on 2,4-Dinitrophenylhydrazine Induced Anaemic Rats
title_sort effect of methanolic leaves extract of telfairia occidentalis on 2 4 dinitrophenylhydrazine induced anaemic rats
topic Anemia
Telfairia occidentalis
2, 4-dinitrophenylhydrazine
Haematological parameters
url https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/34
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