Early growth response gene-2 (Egr-2) regulates the development of B and T cells.
<h4>Background</h4>Understanding of how transcription factors are involved in lymphocyte development still remains a challenge. It has been shown that Egr-2 deficiency results in impaired NKT cell development and defective positive selection of T cells. Here we investigated the developme...
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Public Library of Science (PLoS)
2011-04-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0018498&type=printable |
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| author | Suling Li Alistair L J Symonds Bo Zhu Mengya Liu Meera V Raymond Tizong Miao Ping Wang |
| author_facet | Suling Li Alistair L J Symonds Bo Zhu Mengya Liu Meera V Raymond Tizong Miao Ping Wang |
| author_sort | Suling Li |
| collection | DOAJ |
| description | <h4>Background</h4>Understanding of how transcription factors are involved in lymphocyte development still remains a challenge. It has been shown that Egr-2 deficiency results in impaired NKT cell development and defective positive selection of T cells. Here we investigated the development of T, B and NKT cells in Egr-2 transgenic mice and the roles in the regulation of distinct stages of B and T cell development.<h4>Methods and findings</h4>The expression of Egr1, 2 and 3 were analysed at different stages of T and B cell development by RT-PCT and results showed that the expression was strictly regulated at different stages. Forced expression of Egr-2 in CD2(+) lymphocytes resulted in a severe reduction of CD4(+)CD8(+) (DP) cells in thymus and pro-B cells in bone marrow, which was associated with reduced expression of Notch1 in ISP thymocytes and Pax5 in pro-B cells, suggesting that retraction of Egr-2 at the ISP and pro-B cell stages is important for the activation of lineage differentiation programs. In contrast to reduction of DP and pro-B cells, Egr-2 enhanced the maturation of DP cells into single positive (SP) T and NKT cells in thymus, and immature B cells into mature B cells in bone marrow.<h4>Conclusions</h4>Our results demonstrate that Egr-2 expressed in restricted stages of lymphocyte development plays a dynamic, but similar role for the development of T, NKT and B cells. |
| format | Article |
| id | doaj-art-23f18569708346089680a0fc4a3bb7a4 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-04-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-23f18569708346089680a0fc4a3bb7a42025-08-20T03:44:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-04-0164e1849810.1371/journal.pone.0018498Early growth response gene-2 (Egr-2) regulates the development of B and T cells.Suling LiAlistair L J SymondsBo ZhuMengya LiuMeera V RaymondTizong MiaoPing Wang<h4>Background</h4>Understanding of how transcription factors are involved in lymphocyte development still remains a challenge. It has been shown that Egr-2 deficiency results in impaired NKT cell development and defective positive selection of T cells. Here we investigated the development of T, B and NKT cells in Egr-2 transgenic mice and the roles in the regulation of distinct stages of B and T cell development.<h4>Methods and findings</h4>The expression of Egr1, 2 and 3 were analysed at different stages of T and B cell development by RT-PCT and results showed that the expression was strictly regulated at different stages. Forced expression of Egr-2 in CD2(+) lymphocytes resulted in a severe reduction of CD4(+)CD8(+) (DP) cells in thymus and pro-B cells in bone marrow, which was associated with reduced expression of Notch1 in ISP thymocytes and Pax5 in pro-B cells, suggesting that retraction of Egr-2 at the ISP and pro-B cell stages is important for the activation of lineage differentiation programs. In contrast to reduction of DP and pro-B cells, Egr-2 enhanced the maturation of DP cells into single positive (SP) T and NKT cells in thymus, and immature B cells into mature B cells in bone marrow.<h4>Conclusions</h4>Our results demonstrate that Egr-2 expressed in restricted stages of lymphocyte development plays a dynamic, but similar role for the development of T, NKT and B cells.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0018498&type=printable |
| spellingShingle | Suling Li Alistair L J Symonds Bo Zhu Mengya Liu Meera V Raymond Tizong Miao Ping Wang Early growth response gene-2 (Egr-2) regulates the development of B and T cells. PLoS ONE |
| title | Early growth response gene-2 (Egr-2) regulates the development of B and T cells. |
| title_full | Early growth response gene-2 (Egr-2) regulates the development of B and T cells. |
| title_fullStr | Early growth response gene-2 (Egr-2) regulates the development of B and T cells. |
| title_full_unstemmed | Early growth response gene-2 (Egr-2) regulates the development of B and T cells. |
| title_short | Early growth response gene-2 (Egr-2) regulates the development of B and T cells. |
| title_sort | early growth response gene 2 egr 2 regulates the development of b and t cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0018498&type=printable |
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