Molecular surface coverage standards by reference-free GIXRF supporting SERS and SEIRA substrate benchmarking

Non-destructive reference-free grazing incidence X-ray fluorescence (RF-GIXRF) is proposed as a highly effective analytical technique for extracting molecular arrangement density in self-assembled monolayers. The establishment of surface density standards through RF-GIXRF impacts various application...

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Main Authors: Cara Eleonora, Hönicke Philipp, Kayser Yves, Beckhoff Burkhard, Giovannozzi Andrea M., Klapetek Petr, Zoccante Alberto, Cossi Maurizio, Tay Li-Lin, Boarino Luca, Ferrarese Lupi Federico
Format: Article
Language:English
Published: De Gruyter 2024-08-01
Series:Nanophotonics
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Online Access:https://doi.org/10.1515/nanoph-2024-0222
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author Cara Eleonora
Hönicke Philipp
Kayser Yves
Beckhoff Burkhard
Giovannozzi Andrea M.
Klapetek Petr
Zoccante Alberto
Cossi Maurizio
Tay Li-Lin
Boarino Luca
Ferrarese Lupi Federico
author_facet Cara Eleonora
Hönicke Philipp
Kayser Yves
Beckhoff Burkhard
Giovannozzi Andrea M.
Klapetek Petr
Zoccante Alberto
Cossi Maurizio
Tay Li-Lin
Boarino Luca
Ferrarese Lupi Federico
author_sort Cara Eleonora
collection DOAJ
description Non-destructive reference-free grazing incidence X-ray fluorescence (RF-GIXRF) is proposed as a highly effective analytical technique for extracting molecular arrangement density in self-assembled monolayers. The establishment of surface density standards through RF-GIXRF impacts various applications, from calibrating laboratory XRF setups to expanding its applicability in materials science, particularly in surface coating scenarios with molecular assemblies. Accurate determination of coverage density is crucial for proper functionalization and interaction, such as in assessing the surface concentration of probes on plasmonic nanostructures. However, limited synchrotron radiation access hinders widespread use, prompting the need for molecular surface density standards, especially for benchmarking substrates for surface-enhanced Raman and infrared absorption spectroscopies (SERS and SEIRA) as well as associated surface-enhanced techniques. Using reproducible densities on gold ensures a solid evaluation of the number of molecules contributing to enhanced signals, facilitating comparability across substrates. The research discusses the importance of employing molecular surface density standards for advancing the field of surface-enhanced spectroscopies, encouraging collaborative efforts in protocol development and benchmarking in surface science.
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spelling doaj-art-23ebd6e5455c4289867faaea026d3d7b2025-08-20T02:38:26ZengDe GruyterNanophotonics2192-86142024-08-0113254605461410.1515/nanoph-2024-0222Molecular surface coverage standards by reference-free GIXRF supporting SERS and SEIRA substrate benchmarkingCara Eleonora0Hönicke Philipp1Kayser Yves2Beckhoff Burkhard3Giovannozzi Andrea M.4Klapetek Petr5Zoccante Alberto6Cossi Maurizio7Tay Li-Lin8Boarino Luca9Ferrarese Lupi Federico10Istituto Nazionale di Ricerca Metrologica, Torino, ItalyX-ray Spectrometry, Physikalisch-Technische Bundesanstalt, Berlin, GermanyX-ray Spectrometry, Physikalisch-Technische Bundesanstalt, Berlin, GermanyX-ray Spectrometry, Physikalisch-Technische Bundesanstalt, Berlin, GermanyIstituto Nazionale di Ricerca Metrologica, Torino, ItalyCzech Metrology Institute, Brno, Czech RepublicDipartimento di Scienze e Innovazione Tecnologica, Università degli Studi del Piemonte Orientale Amedeo Avogadro Sede di Alessandria, Alessandria, ItalyDipartimento di Scienze e Innovazione Tecnologica, Università degli Studi del Piemonte Orientale Amedeo Avogadro Sede di Alessandria, Alessandria, ItalyNRC, Ottawa, CanadaIstituto Nazionale di Ricerca Metrologica, Torino, ItalyIstituto Nazionale di Ricerca Metrologica, Torino, ItalyNon-destructive reference-free grazing incidence X-ray fluorescence (RF-GIXRF) is proposed as a highly effective analytical technique for extracting molecular arrangement density in self-assembled monolayers. The establishment of surface density standards through RF-GIXRF impacts various applications, from calibrating laboratory XRF setups to expanding its applicability in materials science, particularly in surface coating scenarios with molecular assemblies. Accurate determination of coverage density is crucial for proper functionalization and interaction, such as in assessing the surface concentration of probes on plasmonic nanostructures. However, limited synchrotron radiation access hinders widespread use, prompting the need for molecular surface density standards, especially for benchmarking substrates for surface-enhanced Raman and infrared absorption spectroscopies (SERS and SEIRA) as well as associated surface-enhanced techniques. Using reproducible densities on gold ensures a solid evaluation of the number of molecules contributing to enhanced signals, facilitating comparability across substrates. The research discusses the importance of employing molecular surface density standards for advancing the field of surface-enhanced spectroscopies, encouraging collaborative efforts in protocol development and benchmarking in surface science.https://doi.org/10.1515/nanoph-2024-0222self-assembled monolayergrazing-incidence x-ray fluorescencesers seiramolecular surface densitysurface coverage standards
spellingShingle Cara Eleonora
Hönicke Philipp
Kayser Yves
Beckhoff Burkhard
Giovannozzi Andrea M.
Klapetek Petr
Zoccante Alberto
Cossi Maurizio
Tay Li-Lin
Boarino Luca
Ferrarese Lupi Federico
Molecular surface coverage standards by reference-free GIXRF supporting SERS and SEIRA substrate benchmarking
Nanophotonics
self-assembled monolayer
grazing-incidence x-ray fluorescence
sers seira
molecular surface density
surface coverage standards
title Molecular surface coverage standards by reference-free GIXRF supporting SERS and SEIRA substrate benchmarking
title_full Molecular surface coverage standards by reference-free GIXRF supporting SERS and SEIRA substrate benchmarking
title_fullStr Molecular surface coverage standards by reference-free GIXRF supporting SERS and SEIRA substrate benchmarking
title_full_unstemmed Molecular surface coverage standards by reference-free GIXRF supporting SERS and SEIRA substrate benchmarking
title_short Molecular surface coverage standards by reference-free GIXRF supporting SERS and SEIRA substrate benchmarking
title_sort molecular surface coverage standards by reference free gixrf supporting sers and seira substrate benchmarking
topic self-assembled monolayer
grazing-incidence x-ray fluorescence
sers seira
molecular surface density
surface coverage standards
url https://doi.org/10.1515/nanoph-2024-0222
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