Low Molecular Weight Fucoidan Alleviates Cardiac Dysfunction in Diabetic Goto-Kakizaki Rats by Reducing Oxidative Stress and Cardiomyocyte Apoptosis
Diabetic cardiomyopathy (DCM) is characterized by cardiac dysfunction and cardiomyocyte apoptosis. Oxidative stress is suggested to be the major contributor to the development of DCM. This study was intended to evaluate the protective effect of low molecular weight fucoidan (LMWF) against cardiac dy...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2014/420929 |
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| author | Xinfeng Yu Quanbin Zhang Wentong Cui Zheng Zeng Wenzhe Yang Chao Zhang Hongwei Zhao Weidong Gao Xiaomin Wang Dali Luo |
| author_facet | Xinfeng Yu Quanbin Zhang Wentong Cui Zheng Zeng Wenzhe Yang Chao Zhang Hongwei Zhao Weidong Gao Xiaomin Wang Dali Luo |
| author_sort | Xinfeng Yu |
| collection | DOAJ |
| description | Diabetic cardiomyopathy (DCM) is characterized by cardiac dysfunction and cardiomyocyte apoptosis. Oxidative stress is suggested to be the major contributor to the development of DCM. This study was intended to evaluate the protective effect of low molecular weight fucoidan (LMWF) against cardiac dysfunction in diabetic rats. Type 2 diabetic goto-kakizaki rats were untreated or treated with LMWF (50 and 100 mg/kg/day) for three months. The establishment of DCM model and the effects of LMWF on cardiac function were evaluated by echocardiography and isolated heart perfusion. Ventricle staining with H-E or Sirius Red was performed to investigate the structural changes in myocardium. Functional evaluation demonstrated that LMWF has a beneficial effect on DCM by enhancing myocardial contractility and mitigating cardiac fibrosis. Additionally, LMWF exerted significant inhibitory effects on the reactive oxygen species production and myocyte apoptosis in diabetic hearts. The depressed activity of superoxide dismutase in diabetic heart was also improved by intervention with LMWF. Moreover, LMWF robustly inhibited the enhanced expression of protein kinase C β, an important contributor to oxidative stress, in diabetic heart and high glucose-treated cardiomyocytes. In conclusion, LMWF possesses a protective effect against DCM through ameliorations of PKCβ-mediated oxidative stress and subsequent cardiomyocyte apoptosis in diabetes. |
| format | Article |
| id | doaj-art-23eabae009944f2ebb603505696b4664 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-23eabae009944f2ebb603505696b46642025-08-20T03:54:58ZengWileyJournal of Diabetes Research2314-67452314-67532014-01-01201410.1155/2014/420929420929Low Molecular Weight Fucoidan Alleviates Cardiac Dysfunction in Diabetic Goto-Kakizaki Rats by Reducing Oxidative Stress and Cardiomyocyte ApoptosisXinfeng Yu0Quanbin Zhang1Wentong Cui2Zheng Zeng3Wenzhe Yang4Chao Zhang5Hongwei Zhao6Weidong Gao7Xiaomin Wang8Dali Luo9Department of Pharmacology, School of Chemical Biology & Pharmaceutical Sciences, Capital Medical University, Youanmenwai Street, No. 10 Xitoutiao, Fengtai District, Beijing 100069, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaDepartment of Pharmacology, School of Chemical Biology & Pharmaceutical Sciences, Capital Medical University, Youanmenwai Street, No. 10 Xitoutiao, Fengtai District, Beijing 100069, ChinaDepartment of Pharmacology, School of Chemical Biology & Pharmaceutical Sciences, Capital Medical University, Youanmenwai Street, No. 10 Xitoutiao, Fengtai District, Beijing 100069, ChinaDepartment of Pharmacology, School of Chemical Biology & Pharmaceutical Sciences, Capital Medical University, Youanmenwai Street, No. 10 Xitoutiao, Fengtai District, Beijing 100069, ChinaDepartment of Pharmacology, School of Chemical Biology & Pharmaceutical Sciences, Capital Medical University, Youanmenwai Street, No. 10 Xitoutiao, Fengtai District, Beijing 100069, ChinaDepartment of Pharmacology, School of Chemical Biology & Pharmaceutical Sciences, Capital Medical University, Youanmenwai Street, No. 10 Xitoutiao, Fengtai District, Beijing 100069, ChinaDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USADepartment of Physiology, Capital Medical University, Beijing 100069, ChinaDepartment of Pharmacology, School of Chemical Biology & Pharmaceutical Sciences, Capital Medical University, Youanmenwai Street, No. 10 Xitoutiao, Fengtai District, Beijing 100069, ChinaDiabetic cardiomyopathy (DCM) is characterized by cardiac dysfunction and cardiomyocyte apoptosis. Oxidative stress is suggested to be the major contributor to the development of DCM. This study was intended to evaluate the protective effect of low molecular weight fucoidan (LMWF) against cardiac dysfunction in diabetic rats. Type 2 diabetic goto-kakizaki rats were untreated or treated with LMWF (50 and 100 mg/kg/day) for three months. The establishment of DCM model and the effects of LMWF on cardiac function were evaluated by echocardiography and isolated heart perfusion. Ventricle staining with H-E or Sirius Red was performed to investigate the structural changes in myocardium. Functional evaluation demonstrated that LMWF has a beneficial effect on DCM by enhancing myocardial contractility and mitigating cardiac fibrosis. Additionally, LMWF exerted significant inhibitory effects on the reactive oxygen species production and myocyte apoptosis in diabetic hearts. The depressed activity of superoxide dismutase in diabetic heart was also improved by intervention with LMWF. Moreover, LMWF robustly inhibited the enhanced expression of protein kinase C β, an important contributor to oxidative stress, in diabetic heart and high glucose-treated cardiomyocytes. In conclusion, LMWF possesses a protective effect against DCM through ameliorations of PKCβ-mediated oxidative stress and subsequent cardiomyocyte apoptosis in diabetes.http://dx.doi.org/10.1155/2014/420929 |
| spellingShingle | Xinfeng Yu Quanbin Zhang Wentong Cui Zheng Zeng Wenzhe Yang Chao Zhang Hongwei Zhao Weidong Gao Xiaomin Wang Dali Luo Low Molecular Weight Fucoidan Alleviates Cardiac Dysfunction in Diabetic Goto-Kakizaki Rats by Reducing Oxidative Stress and Cardiomyocyte Apoptosis Journal of Diabetes Research |
| title | Low Molecular Weight Fucoidan Alleviates Cardiac Dysfunction in Diabetic Goto-Kakizaki Rats by Reducing Oxidative Stress and Cardiomyocyte Apoptosis |
| title_full | Low Molecular Weight Fucoidan Alleviates Cardiac Dysfunction in Diabetic Goto-Kakizaki Rats by Reducing Oxidative Stress and Cardiomyocyte Apoptosis |
| title_fullStr | Low Molecular Weight Fucoidan Alleviates Cardiac Dysfunction in Diabetic Goto-Kakizaki Rats by Reducing Oxidative Stress and Cardiomyocyte Apoptosis |
| title_full_unstemmed | Low Molecular Weight Fucoidan Alleviates Cardiac Dysfunction in Diabetic Goto-Kakizaki Rats by Reducing Oxidative Stress and Cardiomyocyte Apoptosis |
| title_short | Low Molecular Weight Fucoidan Alleviates Cardiac Dysfunction in Diabetic Goto-Kakizaki Rats by Reducing Oxidative Stress and Cardiomyocyte Apoptosis |
| title_sort | low molecular weight fucoidan alleviates cardiac dysfunction in diabetic goto kakizaki rats by reducing oxidative stress and cardiomyocyte apoptosis |
| url | http://dx.doi.org/10.1155/2014/420929 |
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