Advancing Semiochemical Tools for Mountain Pine Beetle Management: <i>Dendroctonus ponderosae</i> Responses to Saprophytic Fungal Volatiles

Background/Objectives: Within their host trees, mountain pine beetles (MPBs, <i>Dendroctonus ponderosae</i>) interact with many fungal species, each releasing a unique profile of volatile organic compounds (VOCs). The FVOCs released by the two primary symbionts of MPBs, <i>Grosmann...

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Main Authors: Leah Crandall, Rashaduz Zaman, Guncha Ishangulyyeva, Nadir Erbilgin
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Metabolites
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Online Access:https://www.mdpi.com/2218-1989/15/7/488
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author Leah Crandall
Rashaduz Zaman
Guncha Ishangulyyeva
Nadir Erbilgin
author_facet Leah Crandall
Rashaduz Zaman
Guncha Ishangulyyeva
Nadir Erbilgin
author_sort Leah Crandall
collection DOAJ
description Background/Objectives: Within their host trees, mountain pine beetles (MPBs, <i>Dendroctonus ponderosae</i>) interact with many fungal species, each releasing a unique profile of volatile organic compounds (VOCs). The FVOCs released by the two primary symbionts of MPBs, <i>Grosmannia clavigera</i> and <i>Ophiostoma montium</i>, have been found to enhance MPB attraction in the field and laboratory studies. Opportunistic, saprophytic fungal species, such as <i>Aspergillus</i> sp. and <i>Trichoderma atroviride</i>, are also common in MPB galleries and can negatively impact MPB fitness. However, little is known about the FVOCs produced by these fungal species and how they may impact MPB feeding and attraction. Methods: To address this knowledge gap, we characterized the FVOC profile of <i>T. atroviride</i>, and performed bioassays to test the effects of its FVOCs on MPB attraction and feeding activity. Results: Our chemical analysis revealed several FVOCs from <i>T. atroviride</i> known to inhibit the growth of competing fungal species and impact subcortical-beetle attraction. Conclusions: From those FVOCs, we recommended four compounds—2-pentanone, 2-heptanone, 2-pentanol, and phenylethyl alcohol—for use in future field tests as anti-attraction lures for MPBs. In bioassays, we also observed strong MPB repellency from FVOCs released by <i>T. atroviride</i>, as well as the mild effects of FVOCs on MPB feeding activity. Our findings highlight the potential for these FVOCs to be utilized in the development of more effective MPB anti-attractant lures, which are crucial for the monitoring and management of low-density MPB populations.
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spelling doaj-art-23e8e2af7f6641e4b67334a85f5660032025-08-20T03:58:27ZengMDPI AGMetabolites2218-19892025-07-0115748810.3390/metabo15070488Advancing Semiochemical Tools for Mountain Pine Beetle Management: <i>Dendroctonus ponderosae</i> Responses to Saprophytic Fungal VolatilesLeah Crandall0Rashaduz Zaman1Guncha Ishangulyyeva2Nadir Erbilgin3Department of Renewable Resources, University of Alberta, Edmonton, AB T6G 2H1, CanadaDepartment of Renewable Resources, University of Alberta, Edmonton, AB T6G 2H1, CanadaDepartment of Renewable Resources, University of Alberta, Edmonton, AB T6G 2H1, CanadaDepartment of Renewable Resources, University of Alberta, Edmonton, AB T6G 2H1, CanadaBackground/Objectives: Within their host trees, mountain pine beetles (MPBs, <i>Dendroctonus ponderosae</i>) interact with many fungal species, each releasing a unique profile of volatile organic compounds (VOCs). The FVOCs released by the two primary symbionts of MPBs, <i>Grosmannia clavigera</i> and <i>Ophiostoma montium</i>, have been found to enhance MPB attraction in the field and laboratory studies. Opportunistic, saprophytic fungal species, such as <i>Aspergillus</i> sp. and <i>Trichoderma atroviride</i>, are also common in MPB galleries and can negatively impact MPB fitness. However, little is known about the FVOCs produced by these fungal species and how they may impact MPB feeding and attraction. Methods: To address this knowledge gap, we characterized the FVOC profile of <i>T. atroviride</i>, and performed bioassays to test the effects of its FVOCs on MPB attraction and feeding activity. Results: Our chemical analysis revealed several FVOCs from <i>T. atroviride</i> known to inhibit the growth of competing fungal species and impact subcortical-beetle attraction. Conclusions: From those FVOCs, we recommended four compounds—2-pentanone, 2-heptanone, 2-pentanol, and phenylethyl alcohol—for use in future field tests as anti-attraction lures for MPBs. In bioassays, we also observed strong MPB repellency from FVOCs released by <i>T. atroviride</i>, as well as the mild effects of FVOCs on MPB feeding activity. Our findings highlight the potential for these FVOCs to be utilized in the development of more effective MPB anti-attractant lures, which are crucial for the monitoring and management of low-density MPB populations.https://www.mdpi.com/2218-1989/15/7/488bark beetlesforest healthinsect behaviour modulationmicrobial emissionspopulation monitoringvolatile-mediated interaction
spellingShingle Leah Crandall
Rashaduz Zaman
Guncha Ishangulyyeva
Nadir Erbilgin
Advancing Semiochemical Tools for Mountain Pine Beetle Management: <i>Dendroctonus ponderosae</i> Responses to Saprophytic Fungal Volatiles
Metabolites
bark beetles
forest health
insect behaviour modulation
microbial emissions
population monitoring
volatile-mediated interaction
title Advancing Semiochemical Tools for Mountain Pine Beetle Management: <i>Dendroctonus ponderosae</i> Responses to Saprophytic Fungal Volatiles
title_full Advancing Semiochemical Tools for Mountain Pine Beetle Management: <i>Dendroctonus ponderosae</i> Responses to Saprophytic Fungal Volatiles
title_fullStr Advancing Semiochemical Tools for Mountain Pine Beetle Management: <i>Dendroctonus ponderosae</i> Responses to Saprophytic Fungal Volatiles
title_full_unstemmed Advancing Semiochemical Tools for Mountain Pine Beetle Management: <i>Dendroctonus ponderosae</i> Responses to Saprophytic Fungal Volatiles
title_short Advancing Semiochemical Tools for Mountain Pine Beetle Management: <i>Dendroctonus ponderosae</i> Responses to Saprophytic Fungal Volatiles
title_sort advancing semiochemical tools for mountain pine beetle management i dendroctonus ponderosae i responses to saprophytic fungal volatiles
topic bark beetles
forest health
insect behaviour modulation
microbial emissions
population monitoring
volatile-mediated interaction
url https://www.mdpi.com/2218-1989/15/7/488
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AT gunchaishangulyyeva advancingsemiochemicaltoolsformountainpinebeetlemanagementidendroctonusponderosaeiresponsestosaprophyticfungalvolatiles
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