Engineering Komagataella phaffii for ethylene glycol production from xylose
Abstract Ethylene glycol (EG) is a versatile molecule produced in the petrochemical industry and is widely used to manufacture plastic polymers, anti-freeze, and automotive fluids. Biotechnological production of EG from xylose, a pentose present in lignocellulose biomass hydrolysates, has been achie...
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SpringerOpen
2024-11-01
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| Online Access: | https://doi.org/10.1186/s13568-024-01795-0 |
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| author | Clara Vida G. C. Carneiro Débora Trichez Jessica C. Bergmann Viviane Castelo Branco Reis Nils Wagner Thomas Walther João Ricardo Moreira de Almeida |
| author_facet | Clara Vida G. C. Carneiro Débora Trichez Jessica C. Bergmann Viviane Castelo Branco Reis Nils Wagner Thomas Walther João Ricardo Moreira de Almeida |
| author_sort | Clara Vida G. C. Carneiro |
| collection | DOAJ |
| description | Abstract Ethylene glycol (EG) is a versatile molecule produced in the petrochemical industry and is widely used to manufacture plastic polymers, anti-freeze, and automotive fluids. Biotechnological production of EG from xylose, a pentose present in lignocellulose biomass hydrolysates, has been achieved by the engineering of bacteria, such as Escherichia coli and Enterobacter cloacae, and the yeast Saccharomyces cerevisiae with synthetic pathways. In the present work, the Dahms pathway was employed to construct Komagataella phaffii strains capable of producing EG from xylose. Different combinations of the four enzymes that compose the synthetic pathway, namely, xylose dehydrogenase, xylonate dehydratase, dehydro-deoxy-xylonate aldolase, and glycolaldehyde reductase, were successfully expressed in K. phaffii. Increased production of EG (1.31 g/L) was achieved by employing a newly identified xylonate dehydratase (xylD-HL). This xylonate dehydratase allowed 30% higher EG production than a previously known xylonate dehydratase (xylD-CC). Further strain engineering demonstrated that K. phaffii possesses native glycolaldehyde reduction and oxidation activities, which lead to pathway deviation from EG to glycolic acid (GA) production. Finally, cultivation conditions that favor the production of EG over GA were determined. |
| format | Article |
| id | doaj-art-23e45b6dd4df4b3692ae6d2d825ccc9f |
| institution | DOAJ |
| issn | 2191-0855 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | SpringerOpen |
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| spelling | doaj-art-23e45b6dd4df4b3692ae6d2d825ccc9f2025-08-20T02:49:18ZengSpringerOpenAMB Express2191-08552024-11-0114111410.1186/s13568-024-01795-0Engineering Komagataella phaffii for ethylene glycol production from xyloseClara Vida G. C. Carneiro0Débora Trichez1Jessica C. Bergmann2Viviane Castelo Branco Reis3Nils Wagner4Thomas Walther5João Ricardo Moreira de Almeida6Graduate Program of Microbial Biology, Institute of Biology, University of BrasíliaMicrobial Genetics and Biotechnology Laboratory, Embrapa AgroenergyMicrobial Genetics and Biotechnology Laboratory, Embrapa AgroenergyMicrobial Genetics and Biotechnology Laboratory, Embrapa AgroenergyInstitute of Natural Materials Technology, TU DresdenInstitute of Natural Materials Technology, TU DresdenGraduate Program of Microbial Biology, Institute of Biology, University of BrasíliaAbstract Ethylene glycol (EG) is a versatile molecule produced in the petrochemical industry and is widely used to manufacture plastic polymers, anti-freeze, and automotive fluids. Biotechnological production of EG from xylose, a pentose present in lignocellulose biomass hydrolysates, has been achieved by the engineering of bacteria, such as Escherichia coli and Enterobacter cloacae, and the yeast Saccharomyces cerevisiae with synthetic pathways. In the present work, the Dahms pathway was employed to construct Komagataella phaffii strains capable of producing EG from xylose. Different combinations of the four enzymes that compose the synthetic pathway, namely, xylose dehydrogenase, xylonate dehydratase, dehydro-deoxy-xylonate aldolase, and glycolaldehyde reductase, were successfully expressed in K. phaffii. Increased production of EG (1.31 g/L) was achieved by employing a newly identified xylonate dehydratase (xylD-HL). This xylonate dehydratase allowed 30% higher EG production than a previously known xylonate dehydratase (xylD-CC). Further strain engineering demonstrated that K. phaffii possesses native glycolaldehyde reduction and oxidation activities, which lead to pathway deviation from EG to glycolic acid (GA) production. Finally, cultivation conditions that favor the production of EG over GA were determined.https://doi.org/10.1186/s13568-024-01795-0Ethylene glycolXyloseSynthetic biologyMetabolic engineeringK. Phaffii |
| spellingShingle | Clara Vida G. C. Carneiro Débora Trichez Jessica C. Bergmann Viviane Castelo Branco Reis Nils Wagner Thomas Walther João Ricardo Moreira de Almeida Engineering Komagataella phaffii for ethylene glycol production from xylose AMB Express Ethylene glycol Xylose Synthetic biology Metabolic engineering K. Phaffii |
| title | Engineering Komagataella phaffii for ethylene glycol production from xylose |
| title_full | Engineering Komagataella phaffii for ethylene glycol production from xylose |
| title_fullStr | Engineering Komagataella phaffii for ethylene glycol production from xylose |
| title_full_unstemmed | Engineering Komagataella phaffii for ethylene glycol production from xylose |
| title_short | Engineering Komagataella phaffii for ethylene glycol production from xylose |
| title_sort | engineering komagataella phaffii for ethylene glycol production from xylose |
| topic | Ethylene glycol Xylose Synthetic biology Metabolic engineering K. Phaffii |
| url | https://doi.org/10.1186/s13568-024-01795-0 |
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