Real-world outdoor air exposure effects in a model of the human airway epithelium – A comparison of healthy and asthmatic individuals using a mobile laboratory setting
We developed a mobile laboratory allowing field exposure of lung tissue models to ambient air at localities with various pollution sources (Background, Industrial, Traffic, Urban) in different seasons (summer/fall/winter). In samples originating from healthy and asthmatic individuals, we assessed th...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324015719 |
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| author | Pavel Rossner Helena Libalova Tereza Cervena Michal Sima Zuzana Simova Kristyna Vrbova Antonin Ambroz Zuzana Novakova Fatima Elzeinova Anezka Vimrova Lubos Dittrich Michal Vojtisek Martin Pechout Michal Vojtisek-Lom |
| author_facet | Pavel Rossner Helena Libalova Tereza Cervena Michal Sima Zuzana Simova Kristyna Vrbova Antonin Ambroz Zuzana Novakova Fatima Elzeinova Anezka Vimrova Lubos Dittrich Michal Vojtisek Martin Pechout Michal Vojtisek-Lom |
| author_sort | Pavel Rossner |
| collection | DOAJ |
| description | We developed a mobile laboratory allowing field exposure of lung tissue models to ambient air at localities with various pollution sources (Background, Industrial, Traffic, Urban) in different seasons (summer/fall/winter). In samples originating from healthy and asthmatic individuals, we assessed the parameters of toxicity, lipid peroxidation and immune response; we further performed comprehensive monitoring of air pollutants at sampling sites. We measured lactate dehydrogenase (LDH) and adenylate kinase (AK) production and transepithelial electrical resistance (TEER), analyzed 15-F2t-isopostane (IsoP) and a panel of 20 cytokines/chemokines/growth factors. In the ambient air, we detected particulate matter (PM), and other relevant chemicals (benzene, benzo[a]pyrene (BaP), NOx). In the Traffic locality, we found very high concentrations of ultrafine particles and NOx and observed low TEER values in the exposed samples, indicating significant traffic-related toxicity of the ambient air. In the Urban locality, sampled in winter, we observed high PM and BaP levels. We found lower AK levels in samples from healthy individuals exposed in this locality than in the asthmatic samples. In the samples from the Industrial locality, sampled in summer, we detected higher concentrations of TNFα, MIP-1α, Eotaxin, GROα, GM-CSF, IL-6 and IL-7 than in the Urban locality samples. We hypothesize that pollen or other plant-related components of the ambient air were responsible for this response. In conclusion, our data proved the feasibility of our mobile laboratory for field measurements of the biological response of lung tissue models exposed to ambient air, reflecting not only the levels of toxic compounds, but also season-specific parameters. |
| format | Article |
| id | doaj-art-23e13b083413435588d5e077759e9e2a |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-23e13b083413435588d5e077759e9e2a2025-01-23T05:25:46ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117495Real-world outdoor air exposure effects in a model of the human airway epithelium – A comparison of healthy and asthmatic individuals using a mobile laboratory settingPavel Rossner0Helena Libalova1Tereza Cervena2Michal Sima3Zuzana Simova4Kristyna Vrbova5Antonin Ambroz6Zuzana Novakova7Fatima Elzeinova8Anezka Vimrova9Lubos Dittrich10Michal Vojtisek11Martin Pechout12Michal Vojtisek-Lom13Department of Toxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Prague, Czech Republic; Correspondence to: Videnska 1083, Prague 14200, Czech Republic.Department of Toxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Prague, Czech RepublicDepartment of Toxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Prague, Czech RepublicDepartment of Toxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Prague, Czech RepublicDepartment of Toxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Prague, Czech RepublicDepartment of Toxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Prague, Czech RepublicDepartment of Toxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Prague, Czech RepublicDepartment of Toxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Prague, Czech RepublicDepartment of Toxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Prague, Czech RepublicDepartment of Toxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Prague, Czech RepublicFaculty of Mechatronics, Informatics and Interdisciplinary Studies, Technical University of Liberec, Liberec, Czech RepublicFaculty of Mechatronics, Informatics and Interdisciplinary Studies, Technical University of Liberec, Liberec, Czech RepublicFaculty of Mechatronics, Informatics and Interdisciplinary Studies, Technical University of Liberec, Liberec, Czech RepublicFaculty of Mechatronics, Informatics and Interdisciplinary Studies, Technical University of Liberec, Liberec, Czech RepublicWe developed a mobile laboratory allowing field exposure of lung tissue models to ambient air at localities with various pollution sources (Background, Industrial, Traffic, Urban) in different seasons (summer/fall/winter). In samples originating from healthy and asthmatic individuals, we assessed the parameters of toxicity, lipid peroxidation and immune response; we further performed comprehensive monitoring of air pollutants at sampling sites. We measured lactate dehydrogenase (LDH) and adenylate kinase (AK) production and transepithelial electrical resistance (TEER), analyzed 15-F2t-isopostane (IsoP) and a panel of 20 cytokines/chemokines/growth factors. In the ambient air, we detected particulate matter (PM), and other relevant chemicals (benzene, benzo[a]pyrene (BaP), NOx). In the Traffic locality, we found very high concentrations of ultrafine particles and NOx and observed low TEER values in the exposed samples, indicating significant traffic-related toxicity of the ambient air. In the Urban locality, sampled in winter, we observed high PM and BaP levels. We found lower AK levels in samples from healthy individuals exposed in this locality than in the asthmatic samples. In the samples from the Industrial locality, sampled in summer, we detected higher concentrations of TNFα, MIP-1α, Eotaxin, GROα, GM-CSF, IL-6 and IL-7 than in the Urban locality samples. We hypothesize that pollen or other plant-related components of the ambient air were responsible for this response. In conclusion, our data proved the feasibility of our mobile laboratory for field measurements of the biological response of lung tissue models exposed to ambient air, reflecting not only the levels of toxic compounds, but also season-specific parameters.http://www.sciencedirect.com/science/article/pii/S0147651324015719outdoor air pollutionlung tissue modelasthmareal-world exposureair-liquid interface |
| spellingShingle | Pavel Rossner Helena Libalova Tereza Cervena Michal Sima Zuzana Simova Kristyna Vrbova Antonin Ambroz Zuzana Novakova Fatima Elzeinova Anezka Vimrova Lubos Dittrich Michal Vojtisek Martin Pechout Michal Vojtisek-Lom Real-world outdoor air exposure effects in a model of the human airway epithelium – A comparison of healthy and asthmatic individuals using a mobile laboratory setting Ecotoxicology and Environmental Safety outdoor air pollution lung tissue model asthma real-world exposure air-liquid interface |
| title | Real-world outdoor air exposure effects in a model of the human airway epithelium – A comparison of healthy and asthmatic individuals using a mobile laboratory setting |
| title_full | Real-world outdoor air exposure effects in a model of the human airway epithelium – A comparison of healthy and asthmatic individuals using a mobile laboratory setting |
| title_fullStr | Real-world outdoor air exposure effects in a model of the human airway epithelium – A comparison of healthy and asthmatic individuals using a mobile laboratory setting |
| title_full_unstemmed | Real-world outdoor air exposure effects in a model of the human airway epithelium – A comparison of healthy and asthmatic individuals using a mobile laboratory setting |
| title_short | Real-world outdoor air exposure effects in a model of the human airway epithelium – A comparison of healthy and asthmatic individuals using a mobile laboratory setting |
| title_sort | real world outdoor air exposure effects in a model of the human airway epithelium a comparison of healthy and asthmatic individuals using a mobile laboratory setting |
| topic | outdoor air pollution lung tissue model asthma real-world exposure air-liquid interface |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324015719 |
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