Enhancement of Thermal Stability of Ultra-high Temperature-Sterilized Yak Milk by Different Pretreatment Techniques

The enhancing effects of different pre-treatment techniques (addition of emulsifying salts, electrodialysis, and resin adsorption) on the stability of ultra-high temperature (UHT)-sterilized yak milk with standardized protein content was investigated by measurement of ethanol stability, protein prec...

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Main Author: CAO Hongyu, ZHAO Xiaoxuan, WANG Yunna, XIE Ning, LI Xu, PANG Xiaoyang, LÜ Jiaping, YU Jinghua, LI Hongjuan, ZHANG Shuwen
Format: Article
Language:English
Published: China Food Publishing Company 2025-07-01
Series:Shipin Kexue
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Online Access:https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-14-028.pdf
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author CAO Hongyu, ZHAO Xiaoxuan, WANG Yunna, XIE Ning, LI Xu, PANG Xiaoyang, LÜ Jiaping, YU Jinghua, LI Hongjuan, ZHANG Shuwen
author_facet CAO Hongyu, ZHAO Xiaoxuan, WANG Yunna, XIE Ning, LI Xu, PANG Xiaoyang, LÜ Jiaping, YU Jinghua, LI Hongjuan, ZHANG Shuwen
author_sort CAO Hongyu, ZHAO Xiaoxuan, WANG Yunna, XIE Ning, LI Xu, PANG Xiaoyang, LÜ Jiaping, YU Jinghua, LI Hongjuan, ZHANG Shuwen
collection DOAJ
description The enhancing effects of different pre-treatment techniques (addition of emulsifying salts, electrodialysis, and resin adsorption) on the stability of ultra-high temperature (UHT)-sterilized yak milk with standardized protein content was investigated by measurement of ethanol stability, protein precipitation rate, whey protein denaturation rate and turbidity stability index (TSI) as well as by using Fourier transform infrared spectroscopy (FTIR) and inductively coupled plasma mass spectrometry (ICP-MS). The results showed that all three pre-treatment methods significantly improved the heat stability of UHT-sterilized yak milk. Compared with the standardized control group, whey protein denaturation rate decreased by 92.02% and 90.15%, soluble calcium content by 3.94% and 13.93%, and protein precipitation rate by 11.71% and 20.40% in the samples treated by resin and electrodialysis, with an average TSI of 2.94 and 1.33, respectively. Whey protein denaturation rate decreased by 84.69%, and protein precipitation rate increased 18.39% in the emulsifying salt-treated sample. The resin-treated group had the highest absolute value of the zeta potential (22.83 mV), closely followed by the electrodialysis group (19.57 mV), suggesting that electrodialysis also improved the heat stability of UHT-sterilized yak milk. FTIR analysis revealed that after UHT treatment, both resin and electrodialysis groups showed an increase in the relative content of α-helix and a decrease in the relative content of β-sheet. In conclusion, this study demonstrates that adjusting the salt balance of yak milk can enhance its UHT stability, providing important guidance for the processing of UHT yak milk.
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publishDate 2025-07-01
publisher China Food Publishing Company
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spelling doaj-art-23e03a3f41b84ea48e82f0e853d868f92025-08-20T03:25:07ZengChina Food Publishing CompanyShipin Kexue1002-66302025-07-01461426324810.7506/spkx1002-6630-20240927-212Enhancement of Thermal Stability of Ultra-high Temperature-Sterilized Yak Milk by Different Pretreatment TechniquesCAO Hongyu, ZHAO Xiaoxuan, WANG Yunna, XIE Ning, LI Xu, PANG Xiaoyang, LÜ Jiaping, YU Jinghua, LI Hongjuan, ZHANG Shuwen0(1. College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China; 2. Institute of Food Science and Technology, Chinese Academy of Agricultural Science, Beijing 100193, China)The enhancing effects of different pre-treatment techniques (addition of emulsifying salts, electrodialysis, and resin adsorption) on the stability of ultra-high temperature (UHT)-sterilized yak milk with standardized protein content was investigated by measurement of ethanol stability, protein precipitation rate, whey protein denaturation rate and turbidity stability index (TSI) as well as by using Fourier transform infrared spectroscopy (FTIR) and inductively coupled plasma mass spectrometry (ICP-MS). The results showed that all three pre-treatment methods significantly improved the heat stability of UHT-sterilized yak milk. Compared with the standardized control group, whey protein denaturation rate decreased by 92.02% and 90.15%, soluble calcium content by 3.94% and 13.93%, and protein precipitation rate by 11.71% and 20.40% in the samples treated by resin and electrodialysis, with an average TSI of 2.94 and 1.33, respectively. Whey protein denaturation rate decreased by 84.69%, and protein precipitation rate increased 18.39% in the emulsifying salt-treated sample. The resin-treated group had the highest absolute value of the zeta potential (22.83 mV), closely followed by the electrodialysis group (19.57 mV), suggesting that electrodialysis also improved the heat stability of UHT-sterilized yak milk. FTIR analysis revealed that after UHT treatment, both resin and electrodialysis groups showed an increase in the relative content of α-helix and a decrease in the relative content of β-sheet. In conclusion, this study demonstrates that adjusting the salt balance of yak milk can enhance its UHT stability, providing important guidance for the processing of UHT yak milk.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-14-028.pdfyak milk; thermal processing; ion exchange; stability
spellingShingle CAO Hongyu, ZHAO Xiaoxuan, WANG Yunna, XIE Ning, LI Xu, PANG Xiaoyang, LÜ Jiaping, YU Jinghua, LI Hongjuan, ZHANG Shuwen
Enhancement of Thermal Stability of Ultra-high Temperature-Sterilized Yak Milk by Different Pretreatment Techniques
Shipin Kexue
yak milk; thermal processing; ion exchange; stability
title Enhancement of Thermal Stability of Ultra-high Temperature-Sterilized Yak Milk by Different Pretreatment Techniques
title_full Enhancement of Thermal Stability of Ultra-high Temperature-Sterilized Yak Milk by Different Pretreatment Techniques
title_fullStr Enhancement of Thermal Stability of Ultra-high Temperature-Sterilized Yak Milk by Different Pretreatment Techniques
title_full_unstemmed Enhancement of Thermal Stability of Ultra-high Temperature-Sterilized Yak Milk by Different Pretreatment Techniques
title_short Enhancement of Thermal Stability of Ultra-high Temperature-Sterilized Yak Milk by Different Pretreatment Techniques
title_sort enhancement of thermal stability of ultra high temperature sterilized yak milk by different pretreatment techniques
topic yak milk; thermal processing; ion exchange; stability
url https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-14-028.pdf
work_keys_str_mv AT caohongyuzhaoxiaoxuanwangyunnaxieninglixupangxiaoyanglujiapingyujinghualihongjuanzhangshuwen enhancementofthermalstabilityofultrahightemperaturesterilizedyakmilkbydifferentpretreatmenttechniques