Dimensionality engineering of flower-like bimetallic nanozyme with high peroxidase-activity for naked-eye and on-site detection of acrylamide in thermally processed foods
Acrylamide (AA) is a neurotoxin and carcinogen that formed during the thermal food processing. Conventional quantification techniques are difficult to realize on-site detection of AA. Herein, a flower-like bimetallic FeCu nanozyme (FeCuzyme) sensor and portable platform were developed for naked-eye...
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KeAi Communications Co., Ltd.
2025-02-01
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| Series: | Nano Materials Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589965124000059 |
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| author | Sen Chen Feifan Liu Taimei Cai Rong Wang Fangjian Ning Hailong Peng |
| author_facet | Sen Chen Feifan Liu Taimei Cai Rong Wang Fangjian Ning Hailong Peng |
| author_sort | Sen Chen |
| collection | DOAJ |
| description | Acrylamide (AA) is a neurotoxin and carcinogen that formed during the thermal food processing. Conventional quantification techniques are difficult to realize on-site detection of AA. Herein, a flower-like bimetallic FeCu nanozyme (FeCuzyme) sensor and portable platform were developed for naked-eye and on-site detection of AA. The FeCuzyme was successfully prepared and exhibited flower-like structure with 3D catalytic centers. Fe/Cu atoms were considered as active center and ligand frameworks were used as cofactor, resulting in collaborative substrate-binding features and remarkably peroxidase-like activity. During the catalytic process, the 3,3′,5,5′-tetrame-thylbenzidine (TMB) oxidation can be quenched by glutathione (GSH), and then restored after thiolene Michael addition reaction between GSH and AA. Given the “on–off–on” effect for TMB oxidation and high POD-like activity, FeCuzyme sensor exhibited a wide linear relationship from 0.50 to 18.00 μM (R2 = 0.9987) and high sensitivity (LOD = 0.2360 μM) with high stability. The practical application of FeCuzyme sensor was successfully validated by HPLC method. Furthermore, a FeCuzyme portable platform was designed with smartphone/laptop, and which can be used for naked-eye and on-site quantitative determination of AA in real food samples. This research provides a way for rational design of a novel nanozyme-based sensing platform for AA detection. |
| format | Article |
| id | doaj-art-23d1a2301d5b447ea44a2980bed8bb42 |
| institution | OA Journals |
| issn | 2589-9651 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Nano Materials Science |
| spelling | doaj-art-23d1a2301d5b447ea44a2980bed8bb422025-08-20T02:16:01ZengKeAi Communications Co., Ltd.Nano Materials Science2589-96512025-02-017112313310.1016/j.nanoms.2024.01.005Dimensionality engineering of flower-like bimetallic nanozyme with high peroxidase-activity for naked-eye and on-site detection of acrylamide in thermally processed foodsSen Chen0Feifan Liu1Taimei Cai2Rong Wang3Fangjian Ning4Hailong Peng5School of Chemistry and Chemical Engineering, Nanchang University, Nanchang, 330031, ChinaSchool of Chemistry and Chemical Engineering, Nanchang University, Nanchang, 330031, ChinaSchool of Life Science, Jiangxi Science and Technology Normal University, Nanchang, 330013, ChinaSchool of Chemistry and Chemical Engineering, Nanchang University, Nanchang, 330031, ChinaChina Food Flavor and Nutrition Health Innovation Center, Beijing Technology and Business University, Beijing, 100048, China; Corresponding author.School of Chemistry and Chemical Engineering, Nanchang University, Nanchang, 330031, China; Corresponding author.Acrylamide (AA) is a neurotoxin and carcinogen that formed during the thermal food processing. Conventional quantification techniques are difficult to realize on-site detection of AA. Herein, a flower-like bimetallic FeCu nanozyme (FeCuzyme) sensor and portable platform were developed for naked-eye and on-site detection of AA. The FeCuzyme was successfully prepared and exhibited flower-like structure with 3D catalytic centers. Fe/Cu atoms were considered as active center and ligand frameworks were used as cofactor, resulting in collaborative substrate-binding features and remarkably peroxidase-like activity. During the catalytic process, the 3,3′,5,5′-tetrame-thylbenzidine (TMB) oxidation can be quenched by glutathione (GSH), and then restored after thiolene Michael addition reaction between GSH and AA. Given the “on–off–on” effect for TMB oxidation and high POD-like activity, FeCuzyme sensor exhibited a wide linear relationship from 0.50 to 18.00 μM (R2 = 0.9987) and high sensitivity (LOD = 0.2360 μM) with high stability. The practical application of FeCuzyme sensor was successfully validated by HPLC method. Furthermore, a FeCuzyme portable platform was designed with smartphone/laptop, and which can be used for naked-eye and on-site quantitative determination of AA in real food samples. This research provides a way for rational design of a novel nanozyme-based sensing platform for AA detection.http://www.sciencedirect.com/science/article/pii/S2589965124000059AcrylamideGlutathioneBimetallic FeCu nanozymeOn-site detectionThermally processed food |
| spellingShingle | Sen Chen Feifan Liu Taimei Cai Rong Wang Fangjian Ning Hailong Peng Dimensionality engineering of flower-like bimetallic nanozyme with high peroxidase-activity for naked-eye and on-site detection of acrylamide in thermally processed foods Nano Materials Science Acrylamide Glutathione Bimetallic FeCu nanozyme On-site detection Thermally processed food |
| title | Dimensionality engineering of flower-like bimetallic nanozyme with high peroxidase-activity for naked-eye and on-site detection of acrylamide in thermally processed foods |
| title_full | Dimensionality engineering of flower-like bimetallic nanozyme with high peroxidase-activity for naked-eye and on-site detection of acrylamide in thermally processed foods |
| title_fullStr | Dimensionality engineering of flower-like bimetallic nanozyme with high peroxidase-activity for naked-eye and on-site detection of acrylamide in thermally processed foods |
| title_full_unstemmed | Dimensionality engineering of flower-like bimetallic nanozyme with high peroxidase-activity for naked-eye and on-site detection of acrylamide in thermally processed foods |
| title_short | Dimensionality engineering of flower-like bimetallic nanozyme with high peroxidase-activity for naked-eye and on-site detection of acrylamide in thermally processed foods |
| title_sort | dimensionality engineering of flower like bimetallic nanozyme with high peroxidase activity for naked eye and on site detection of acrylamide in thermally processed foods |
| topic | Acrylamide Glutathione Bimetallic FeCu nanozyme On-site detection Thermally processed food |
| url | http://www.sciencedirect.com/science/article/pii/S2589965124000059 |
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