Rats born to Brucella abortus infected mothers become latent carriers of Brucella
Introduction: Rats are known to be infected with Brucella. Vertical transmission of brucellosis was recorded in rats. The study was performed to judge whether rats born from Brucella abortus infected mothers can act as latent carriers of Brucella infection. Methodology: Female Sprague Dawley (SD)...
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The Journal of Infection in Developing Countries
2011-11-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/1997 |
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| author | Md. Ariful Islam Mst. Minara Khatun Byeong-Kirl Baek |
| author_facet | Md. Ariful Islam Mst. Minara Khatun Byeong-Kirl Baek |
| author_sort | Md. Ariful Islam |
| collection | DOAJ |
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Introduction: Rats are known to be infected with Brucella. Vertical transmission of brucellosis was recorded in rats. The study was performed to judge whether rats born from Brucella abortus infected mothers can act as latent carriers of Brucella infection.
Methodology: Female Sprague Dawley (SD) rats were experimentally infected with B. abortus biotype 1 and subsequently bred 10 days post infection (PI). Serum samples of rats (n = 48) born from infected dams were tested using the Rose Bengal plate test (RBPT), tube agglutination test (TAT), and enzyme-linked immunosorbent assay (ELISA) at one, two and three months of age. Tissue samples were plated onto Brucella agar and blood agar media and incubated at 37°C with 5% CO2 for five to seven days for isolation of bacteria..
Results: B. abortus was isolated from 18 out of 48 rats born to infected dams, and the isolates were confirmed as B. abortus by AMOS (B. abortus, melitensis, ovis and suis) PCR assay with the production of a 498 bp PCR amplicon. Serum samples of rats (n = 48) born from infected dams were tested negative using the RBPT, TAT and ELISA at all time points.
Conclusion: We conclude from the study that rats born to infected dams may become latent carriers of Brucella infection potentially providing a reservoir for future transmission.
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| format | Article |
| id | doaj-art-23d1772fe82941d49c161da0aa78f968 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2011-11-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-23d1772fe82941d49c161da0aa78f9682025-08-20T02:57:12ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802011-11-0160310.3855/jidc.1997Rats born to Brucella abortus infected mothers become latent carriers of BrucellaMd. Ariful Islam0Mst. Minara Khatun1Byeong-Kirl Baek2Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, BangladeshFaculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, BangladeshCollege of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Introduction: Rats are known to be infected with Brucella. Vertical transmission of brucellosis was recorded in rats. The study was performed to judge whether rats born from Brucella abortus infected mothers can act as latent carriers of Brucella infection. Methodology: Female Sprague Dawley (SD) rats were experimentally infected with B. abortus biotype 1 and subsequently bred 10 days post infection (PI). Serum samples of rats (n = 48) born from infected dams were tested using the Rose Bengal plate test (RBPT), tube agglutination test (TAT), and enzyme-linked immunosorbent assay (ELISA) at one, two and three months of age. Tissue samples were plated onto Brucella agar and blood agar media and incubated at 37°C with 5% CO2 for five to seven days for isolation of bacteria.. Results: B. abortus was isolated from 18 out of 48 rats born to infected dams, and the isolates were confirmed as B. abortus by AMOS (B. abortus, melitensis, ovis and suis) PCR assay with the production of a 498 bp PCR amplicon. Serum samples of rats (n = 48) born from infected dams were tested negative using the RBPT, TAT and ELISA at all time points. Conclusion: We conclude from the study that rats born to infected dams may become latent carriers of Brucella infection potentially providing a reservoir for future transmission. https://jidc.org/index.php/journal/article/view/1997ratsBrucella abortus biotype 1latent carrier |
| spellingShingle | Md. Ariful Islam Mst. Minara Khatun Byeong-Kirl Baek Rats born to Brucella abortus infected mothers become latent carriers of Brucella Journal of Infection in Developing Countries rats Brucella abortus biotype 1 latent carrier |
| title | Rats born to Brucella abortus infected mothers become latent carriers of Brucella |
| title_full | Rats born to Brucella abortus infected mothers become latent carriers of Brucella |
| title_fullStr | Rats born to Brucella abortus infected mothers become latent carriers of Brucella |
| title_full_unstemmed | Rats born to Brucella abortus infected mothers become latent carriers of Brucella |
| title_short | Rats born to Brucella abortus infected mothers become latent carriers of Brucella |
| title_sort | rats born to brucella abortus infected mothers become latent carriers of brucella |
| topic | rats Brucella abortus biotype 1 latent carrier |
| url | https://jidc.org/index.php/journal/article/view/1997 |
| work_keys_str_mv | AT mdarifulislam ratsborntobrucellaabortusinfectedmothersbecomelatentcarriersofbrucella AT mstminarakhatun ratsborntobrucellaabortusinfectedmothersbecomelatentcarriersofbrucella AT byeongkirlbaek ratsborntobrucellaabortusinfectedmothersbecomelatentcarriersofbrucella |