Columbianetin protects from high-glycemic podocyte damage by regulating autophagy
Objective To explore the protective effect of columbianetin（CBT）on the injury of podocytes induced by high glucose and its mechanism.Methods Immortalized murine foot cell line MPC-5 was cultured in vitro and the effects of CBT on injury and autophagy induced by high glucose were observed.Autophagy-r...
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| Language: | zho |
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Editorial Department of Journal of Clinical Nephrology
2021-01-01
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| Series: | Linchuang shenzangbing zazhi |
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| Online Access: | http://www.lcszb.com/thesisDetails?columnId=57904045&Fpath=home&index=0 |
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| author | Shi Lang Song Zhi-xia Zhang Ya-fei |
| author_facet | Shi Lang Song Zhi-xia Zhang Ya-fei |
| author_sort | Shi Lang |
| collection | DOAJ |
| description | Objective To explore the protective effect of columbianetin（CBT）on the injury of podocytes induced by high glucose and its mechanism.Methods Immortalized murine foot cell line MPC-5 was cultured in vitro and the effects of CBT on injury and autophagy induced by high glucose were observed.Autophagy-related proteins were detected by Western blot.A fluorescent microscope was utilized for observing green fluorescent particles of GFP-LC3-Ⅱpodocyte after transfection.Results High glucose induced the injury of podocytes and decreased autophagy.CBT had a significant protective effect on the injury of podocytes induced by high glucose and could up-regulate autophagy.CBT up-regulated the expressions of TLR4 and p-JNK.siRNA TLR4 and inhibitor SP600125 blocked the protective effect of CBT.Conclusion CBT can induce autophagy in podocytes to protect podocytes from damage caused by high glucose.It is mediated by promoting TLR4/p-JNK signaling pathway. |
| format | Article |
| id | doaj-art-23b5d1a9b6cb471db7e965f360c8e4d9 |
| institution | DOAJ |
| issn | 1671-2390 |
| language | zho |
| publishDate | 2021-01-01 |
| publisher | Editorial Department of Journal of Clinical Nephrology |
| record_format | Article |
| series | Linchuang shenzangbing zazhi |
| spelling | doaj-art-23b5d1a9b6cb471db7e965f360c8e4d92025-08-20T03:09:25ZzhoEditorial Department of Journal of Clinical NephrologyLinchuang shenzangbing zazhi1671-23902021-01-0114014657904045Columbianetin protects from high-glycemic podocyte damage by regulating autophagyShi LangSong Zhi-xiaZhang Ya-feiObjective To explore the protective effect of columbianetin（CBT）on the injury of podocytes induced by high glucose and its mechanism.Methods Immortalized murine foot cell line MPC-5 was cultured in vitro and the effects of CBT on injury and autophagy induced by high glucose were observed.Autophagy-related proteins were detected by Western blot.A fluorescent microscope was utilized for observing green fluorescent particles of GFP-LC3-Ⅱpodocyte after transfection.Results High glucose induced the injury of podocytes and decreased autophagy.CBT had a significant protective effect on the injury of podocytes induced by high glucose and could up-regulate autophagy.CBT up-regulated the expressions of TLR4 and p-JNK.siRNA TLR4 and inhibitor SP600125 blocked the protective effect of CBT.Conclusion CBT can induce autophagy in podocytes to protect podocytes from damage caused by high glucose.It is mediated by promoting TLR4/p-JNK signaling pathway.http://www.lcszb.com/thesisDetails?columnId=57904045&Fpath=home&index=0ColumbianetinPodocyteAutophagyDiabetic nephropathy |
| spellingShingle | Shi Lang Song Zhi-xia Zhang Ya-fei Columbianetin protects from high-glycemic podocyte damage by regulating autophagy Linchuang shenzangbing zazhi Columbianetin Podocyte Autophagy Diabetic nephropathy |
| title | Columbianetin protects from high-glycemic podocyte damage by regulating autophagy |
| title_full | Columbianetin protects from high-glycemic podocyte damage by regulating autophagy |
| title_fullStr | Columbianetin protects from high-glycemic podocyte damage by regulating autophagy |
| title_full_unstemmed | Columbianetin protects from high-glycemic podocyte damage by regulating autophagy |
| title_short | Columbianetin protects from high-glycemic podocyte damage by regulating autophagy |
| title_sort | columbianetin protects from high glycemic podocyte damage by regulating autophagy |
| topic | Columbianetin Podocyte Autophagy Diabetic nephropathy |
| url | http://www.lcszb.com/thesisDetails?columnId=57904045&Fpath=home&index=0 |
| work_keys_str_mv | AT shilang columbianetinprotectsfromhighglycemicpodocytedamagebyregulatingautophagy AT songzhixia columbianetinprotectsfromhighglycemicpodocytedamagebyregulatingautophagy AT zhangyafei columbianetinprotectsfromhighglycemicpodocytedamagebyregulatingautophagy |