Whole-genome resequencing revealed the genetic diversity and body weight-related genes of Gannan yak

Body weight (BW) is a crucial indicator of animal growth and development, significantly influencing animal husbandry practices. Previous research has identified several genes associated with BW in certain yak breeds. However, the genetic basis of BW in Gannan yaks has not been reported. In this stud...

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Main Authors: Minghao Zhang, Chun Huang, Qi Bao, Tong Wang, Xiaoming Ma, Guangyao Meng, Xuelan Zhou, Qingbo Zheng, Min Chu, Chunnian Liang, Xian Guo, Pengjia Bao, Ping Yan
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Veterinary Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2025.1540523/full
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author Minghao Zhang
Minghao Zhang
Chun Huang
Chun Huang
Qi Bao
Tong Wang
Tong Wang
Xiaoming Ma
Xiaoming Ma
Guangyao Meng
Guangyao Meng
Xuelan Zhou
Xuelan Zhou
Qingbo Zheng
Qingbo Zheng
Min Chu
Min Chu
Chunnian Liang
Chunnian Liang
Xian Guo
Xian Guo
Pengjia Bao
Pengjia Bao
Ping Yan
Ping Yan
author_facet Minghao Zhang
Minghao Zhang
Chun Huang
Chun Huang
Qi Bao
Tong Wang
Tong Wang
Xiaoming Ma
Xiaoming Ma
Guangyao Meng
Guangyao Meng
Xuelan Zhou
Xuelan Zhou
Qingbo Zheng
Qingbo Zheng
Min Chu
Min Chu
Chunnian Liang
Chunnian Liang
Xian Guo
Xian Guo
Pengjia Bao
Pengjia Bao
Ping Yan
Ping Yan
author_sort Minghao Zhang
collection DOAJ
description Body weight (BW) is a crucial indicator of animal growth and development, significantly influencing animal husbandry practices. Previous research has identified several genes associated with BW in certain yak breeds. However, the genetic basis of BW in Gannan yaks has not been reported. In this study, 309 yaks from six breeds across five provinces in China were sampled. This collection included 247 54-month-old female Gannan yaks, along with 20 Xizang yaks, 15 Muli yaks, 10 Pamir yaks, 9 Bazhou yaks, and 8 Zhongdian yaks. Body weight measurements were recorded for the Gannan yaks. Initial analyses of runs of homozygosity (ROH), nucleotide diversity, and linkage disequilibrium (LD) decay among the six yak breeds revealed that Gannan yaks exhibited the lowest ROH, the highest nucleotide diversity, and the fastest LD decay, indicating rich genetic diversity. Subsequently, a genome-wide association study (GWAS) identified 19 BW-related genes in the Gannan yaks, with PMAIP1, GABBR1, LRPPRC, and PPP1R11 identified as key genes. Genome-wide scanning of Group 1 and Group 2 (detailed in section 2.4) identified 90 genes, and Gene Ontology (GO) analysis highlighted FGF2, SHH, and WNT11 as significantly associated with growth, development, and metabolism. Three overlapping genes were identified between GWAS and genome-wide scans. Further analyses, including nucleotide diversity, LD analysis of significant GWAS sites, allele frequency analysis, and SNP association studies, suggested that DRC1 and SELENOI are novel candidate genes for BW in Gannan yaks. These findings provide a molecular foundation for the genetic improvement of Gannan yaks.
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spelling doaj-art-239eade7927a403b89b05f6b0aa622c42025-08-20T03:30:15ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-06-011210.3389/fvets.2025.15405231540523Whole-genome resequencing revealed the genetic diversity and body weight-related genes of Gannan yakMinghao Zhang0Minghao Zhang1Chun Huang2Chun Huang3Qi Bao4Tong Wang5Tong Wang6Xiaoming Ma7Xiaoming Ma8Guangyao Meng9Guangyao Meng10Xuelan Zhou11Xuelan Zhou12Qingbo Zheng13Qingbo Zheng14Min Chu15Min Chu16Chunnian Liang17Chunnian Liang18Xian Guo19Xian Guo20Pengjia Bao21Pengjia Bao22Ping Yan23Ping Yan24Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi-Omics of MARA, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaBody weight (BW) is a crucial indicator of animal growth and development, significantly influencing animal husbandry practices. Previous research has identified several genes associated with BW in certain yak breeds. However, the genetic basis of BW in Gannan yaks has not been reported. In this study, 309 yaks from six breeds across five provinces in China were sampled. This collection included 247 54-month-old female Gannan yaks, along with 20 Xizang yaks, 15 Muli yaks, 10 Pamir yaks, 9 Bazhou yaks, and 8 Zhongdian yaks. Body weight measurements were recorded for the Gannan yaks. Initial analyses of runs of homozygosity (ROH), nucleotide diversity, and linkage disequilibrium (LD) decay among the six yak breeds revealed that Gannan yaks exhibited the lowest ROH, the highest nucleotide diversity, and the fastest LD decay, indicating rich genetic diversity. Subsequently, a genome-wide association study (GWAS) identified 19 BW-related genes in the Gannan yaks, with PMAIP1, GABBR1, LRPPRC, and PPP1R11 identified as key genes. Genome-wide scanning of Group 1 and Group 2 (detailed in section 2.4) identified 90 genes, and Gene Ontology (GO) analysis highlighted FGF2, SHH, and WNT11 as significantly associated with growth, development, and metabolism. Three overlapping genes were identified between GWAS and genome-wide scans. Further analyses, including nucleotide diversity, LD analysis of significant GWAS sites, allele frequency analysis, and SNP association studies, suggested that DRC1 and SELENOI are novel candidate genes for BW in Gannan yaks. These findings provide a molecular foundation for the genetic improvement of Gannan yaks.https://www.frontiersin.org/articles/10.3389/fvets.2025.1540523/fullGannan yakbody weightgenetic diversitygenome-wide association studygenome-wide scanning
spellingShingle Minghao Zhang
Minghao Zhang
Chun Huang
Chun Huang
Qi Bao
Tong Wang
Tong Wang
Xiaoming Ma
Xiaoming Ma
Guangyao Meng
Guangyao Meng
Xuelan Zhou
Xuelan Zhou
Qingbo Zheng
Qingbo Zheng
Min Chu
Min Chu
Chunnian Liang
Chunnian Liang
Xian Guo
Xian Guo
Pengjia Bao
Pengjia Bao
Ping Yan
Ping Yan
Whole-genome resequencing revealed the genetic diversity and body weight-related genes of Gannan yak
Frontiers in Veterinary Science
Gannan yak
body weight
genetic diversity
genome-wide association study
genome-wide scanning
title Whole-genome resequencing revealed the genetic diversity and body weight-related genes of Gannan yak
title_full Whole-genome resequencing revealed the genetic diversity and body weight-related genes of Gannan yak
title_fullStr Whole-genome resequencing revealed the genetic diversity and body weight-related genes of Gannan yak
title_full_unstemmed Whole-genome resequencing revealed the genetic diversity and body weight-related genes of Gannan yak
title_short Whole-genome resequencing revealed the genetic diversity and body weight-related genes of Gannan yak
title_sort whole genome resequencing revealed the genetic diversity and body weight related genes of gannan yak
topic Gannan yak
body weight
genetic diversity
genome-wide association study
genome-wide scanning
url https://www.frontiersin.org/articles/10.3389/fvets.2025.1540523/full
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