Highly carbapenem-resistant Achromobacter xylosoxidans harboring blaNDM-1 in Myanmar
ABSTRACT Achromobacter xylosoxidans is a multidrug-resistant, non-glucose-fermenting, gram-negative bacterium with intrinsic resistance to many antimicrobial agents. Between 2016 and 2017, five A. xylosoxidans isolates were obtained from five patients at three hospitals in Myanmar. Minimum inhibitor...
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American Society for Microbiology
2025-07-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.00080-25 |
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| author | Maiko Kirikae Satoshi Oshiro Satomi Takei Naeko Mizutani Atsuo Itakura Pan Ei Soe Thi Thi Htoon Swe Setk Htay Htay Tin Teruo Kirikae Tatsuya Tada |
| author_facet | Maiko Kirikae Satoshi Oshiro Satomi Takei Naeko Mizutani Atsuo Itakura Pan Ei Soe Thi Thi Htoon Swe Setk Htay Htay Tin Teruo Kirikae Tatsuya Tada |
| author_sort | Maiko Kirikae |
| collection | DOAJ |
| description | ABSTRACT Achromobacter xylosoxidans is a multidrug-resistant, non-glucose-fermenting, gram-negative bacterium with intrinsic resistance to many antimicrobial agents. Between 2016 and 2017, five A. xylosoxidans isolates were obtained from five patients at three hospitals in Myanmar. Minimum inhibitory concentrations (MICs) against various antimicrobial agents were determined using the microdilution method. Whole genome sequencing was performed with the MiSeq and MinION platforms. Resistance genes and their surrounding structures were identified and compared. All five isolates were resistant to amikacin and aztreonam. Among them, one isolate, MyNCGM749, was resistant to imipenem and meropenem with MICs of 256 µg/mL and amikacin with MIC of >512 µg/mL but intermediate to ciprofloxacin with MIC of 2 µg/mL. The isolate carried blaNDM-1 encoding metallo-β-lactamase, blaPSE-1 encoding extended-spectrum-β-lactamase, and blaOXA-114 (encoding intrinsic -β-lactamase present in A. xylosoxidans), along with five aminoglycoside modification encoding genes including aac(6')-Ib, aph (6)-Id, aph(3'')-Ib, ant(4')-Iib, and aph(3')-VI on its chromosome. The genetic structure surrounding blaNDM-1 contained four IS91 elements identical to those found in carbapenem-resistant Pseudomonas asiatica isolates in Myanmar. This is the first report of A. xylosoxidans in Myanmar. Although A. xylosoxidans harboring blaNDM-1 has been reported in a single strain from India, its genomic details have not been previously described. This study indicates that the blaNDM-1-containing structure flanked by IS91 is spreading among gram-negative, non-glucose-fermenting bacteria in Myanmar and neighboring countries.IMPORTANCEAchromobacter species were originally environmental organisms that became opportunistic pathogens with multidrug resistance. Achromobacter xylosoxidans is associated with nosocomially acquired infections affecting multiple organ systems, including the respiratory and urinary tracts, and, less commonly, the cardiovascular and central nervous systems. To date, carbapenem-resistant A. xylosoxidans carrying carbapenemase-encoding genes has been reported in several countries, including Greece, India, Italy, Japan, Korea, Libya, and the Netherlands. In this molecular epidemiological study on A. xylosoxidans in Myanmar, we identified the genomic structure surrounding blaNDM-1, flanked by IS91. This structure may facilitate the spread of non-glucose-fermenting gram-negative bacteria, such as Achromobacter, Pseudomonas, and Stenotrophomonas species, in Asian countries. |
| format | Article |
| id | doaj-art-239df2c0fab2408dbb844d2c24d3294e |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | American Society for Microbiology |
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| series | Microbiology Spectrum |
| spelling | doaj-art-239df2c0fab2408dbb844d2c24d3294e2025-08-20T03:31:20ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-07-0113710.1128/spectrum.00080-25Highly carbapenem-resistant Achromobacter xylosoxidans harboring blaNDM-1 in MyanmarMaiko Kirikae0Satoshi Oshiro1Satomi Takei2Naeko Mizutani3Atsuo Itakura4Pan Ei Soe5Thi Thi Htoon6Swe Setk7Htay Htay Tin8Teruo Kirikae9Tatsuya Tada10Department of Obstetrics and Gynecology, Juntendo University Faculty of Medicine, Tokyo, JapanJuntendo Advanced Research Institute for Health Science, Juntendo University, Tokyo, JapanDepartment of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, JapanJuntendo Advanced Research Institute for Health Science, Juntendo University, Tokyo, JapanDepartment of Obstetrics and Gynecology, Juntendo University Faculty of Medicine, Tokyo, JapanNational Health Laboratory, Yangon, MyanmarNational Health Laboratory, Yangon, MyanmarNational Health Laboratory, Yangon, MyanmarNational Health Laboratory, Yangon, MyanmarJuntendo Advanced Research Institute for Health Science, Juntendo University, Tokyo, JapanDepartment of Microbiology, Juntendo University Graduate School of Medicine, Tokyo, JapanABSTRACT Achromobacter xylosoxidans is a multidrug-resistant, non-glucose-fermenting, gram-negative bacterium with intrinsic resistance to many antimicrobial agents. Between 2016 and 2017, five A. xylosoxidans isolates were obtained from five patients at three hospitals in Myanmar. Minimum inhibitory concentrations (MICs) against various antimicrobial agents were determined using the microdilution method. Whole genome sequencing was performed with the MiSeq and MinION platforms. Resistance genes and their surrounding structures were identified and compared. All five isolates were resistant to amikacin and aztreonam. Among them, one isolate, MyNCGM749, was resistant to imipenem and meropenem with MICs of 256 µg/mL and amikacin with MIC of >512 µg/mL but intermediate to ciprofloxacin with MIC of 2 µg/mL. The isolate carried blaNDM-1 encoding metallo-β-lactamase, blaPSE-1 encoding extended-spectrum-β-lactamase, and blaOXA-114 (encoding intrinsic -β-lactamase present in A. xylosoxidans), along with five aminoglycoside modification encoding genes including aac(6')-Ib, aph (6)-Id, aph(3'')-Ib, ant(4')-Iib, and aph(3')-VI on its chromosome. The genetic structure surrounding blaNDM-1 contained four IS91 elements identical to those found in carbapenem-resistant Pseudomonas asiatica isolates in Myanmar. This is the first report of A. xylosoxidans in Myanmar. Although A. xylosoxidans harboring blaNDM-1 has been reported in a single strain from India, its genomic details have not been previously described. This study indicates that the blaNDM-1-containing structure flanked by IS91 is spreading among gram-negative, non-glucose-fermenting bacteria in Myanmar and neighboring countries.IMPORTANCEAchromobacter species were originally environmental organisms that became opportunistic pathogens with multidrug resistance. Achromobacter xylosoxidans is associated with nosocomially acquired infections affecting multiple organ systems, including the respiratory and urinary tracts, and, less commonly, the cardiovascular and central nervous systems. To date, carbapenem-resistant A. xylosoxidans carrying carbapenemase-encoding genes has been reported in several countries, including Greece, India, Italy, Japan, Korea, Libya, and the Netherlands. In this molecular epidemiological study on A. xylosoxidans in Myanmar, we identified the genomic structure surrounding blaNDM-1, flanked by IS91. This structure may facilitate the spread of non-glucose-fermenting gram-negative bacteria, such as Achromobacter, Pseudomonas, and Stenotrophomonas species, in Asian countries.https://journals.asm.org/doi/10.1128/spectrum.00080-25Achromobacter xylosoxidansmultidrug resistancecarbapenemaseblaNDM-1 |
| spellingShingle | Maiko Kirikae Satoshi Oshiro Satomi Takei Naeko Mizutani Atsuo Itakura Pan Ei Soe Thi Thi Htoon Swe Setk Htay Htay Tin Teruo Kirikae Tatsuya Tada Highly carbapenem-resistant Achromobacter xylosoxidans harboring blaNDM-1 in Myanmar Microbiology Spectrum Achromobacter xylosoxidans multidrug resistance carbapenemase blaNDM-1 |
| title | Highly carbapenem-resistant Achromobacter xylosoxidans harboring blaNDM-1 in Myanmar |
| title_full | Highly carbapenem-resistant Achromobacter xylosoxidans harboring blaNDM-1 in Myanmar |
| title_fullStr | Highly carbapenem-resistant Achromobacter xylosoxidans harboring blaNDM-1 in Myanmar |
| title_full_unstemmed | Highly carbapenem-resistant Achromobacter xylosoxidans harboring blaNDM-1 in Myanmar |
| title_short | Highly carbapenem-resistant Achromobacter xylosoxidans harboring blaNDM-1 in Myanmar |
| title_sort | highly carbapenem resistant achromobacter xylosoxidans harboring blandm 1 in myanmar |
| topic | Achromobacter xylosoxidans multidrug resistance carbapenemase blaNDM-1 |
| url | https://journals.asm.org/doi/10.1128/spectrum.00080-25 |
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