Hydrotalcites as a Promising Adsorbent for Hemicellulose Hydrolysate Detoxification in Xylitol Production

The worldwide demand for sustainable bioprocesses is undeniable, as well as for research aimed at the biotechnological exploitation of lignocellulosic materials, especially their hemicellulosic fractions rich in xylose. Various bioproducts can be obtained from these fractions, although some bottlene...

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Main Authors: Débora D. V. da Silva, Kelly J. Dussán, Isabela A. L. Costa, Marcus B. S. Forte, Maria G. A. Felipe
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Fermentation
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Online Access:https://www.mdpi.com/2311-5637/11/5/243
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author Débora D. V. da Silva
Kelly J. Dussán
Isabela A. L. Costa
Marcus B. S. Forte
Maria G. A. Felipe
author_facet Débora D. V. da Silva
Kelly J. Dussán
Isabela A. L. Costa
Marcus B. S. Forte
Maria G. A. Felipe
author_sort Débora D. V. da Silva
collection DOAJ
description The worldwide demand for sustainable bioprocesses is undeniable, as well as for research aimed at the biotechnological exploitation of lignocellulosic materials, especially their hemicellulosic fractions rich in xylose. Various bioproducts can be obtained from these fractions, although some bottlenecks still exist, such as the presence in hemicellulosic hydrolysates of compounds that are toxic for microorganisms, which requires a previous step of detoxification to reduce them to non-inhibitory levels. The present investigation proposes the use of hydrotalcites as a new detoxifying agent for the hemicellulosic hydrolysate of sugarcane straw to produce xylitol by <i>Candida tropicalis</i>, aiming at a greater removal of phenolics and less loss of sugars. The design of these experiments was used for factorial effect analysis in a simultaneous way; the influences of pH and temperature were evaluated, considering the detoxification process at different times for both uncalcined and calcined hydrotalcite adsorbents. While for the calcined hydrotalcite, the temperature was the significant factor, for the uncalcined, there was also an influence of pH and little effect on the factors of yield and productivity. The effectiveness of hydrotalcites as demonstrated in this research, mainly regarding the ability to reduce the content of phenolic compounds in hydrolysates with a low loss of sugar content, followed by fermentability to produce xylitol, is a strong requirement for the proposition of these new adsorbents in investigations of the development of sustainable technologies for obtaining bioproducts in a biorefinery context.
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institution Kabale University
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spelling doaj-art-239b6f1be4ef40a8acbbd038157635572025-08-20T03:47:49ZengMDPI AGFermentation2311-56372025-04-0111524310.3390/fermentation11050243Hydrotalcites as a Promising Adsorbent for Hemicellulose Hydrolysate Detoxification in Xylitol ProductionDébora D. V. da Silva0Kelly J. Dussán1Isabela A. L. Costa2Marcus B. S. Forte3Maria G. A. Felipe4Department of Biochemistry and Organic Chemistry, Institute of Chemical, São Paulo State University (Unesp), Araraquara 14800-900, São Paulo, BrazilDepartment of Chemical Engineering, Institute of Chemical, São Paulo State University (Unesp), Araraquara 14800-900, São Paulo, BrazilDepartment of Biotechnology, School of Engineering of Lorena (EEL), University of São Paulo, Lorena 12600-000, São Paulo, BrazilFood Engineering and Technology Department (DETA), Faculty of Food Engineering (FEA), University of Campinas (UNICAMP), Campinas 13083-862, São Paulo, BrazilDepartment of Biotechnology, School of Engineering of Lorena (EEL), University of São Paulo, Lorena 12600-000, São Paulo, BrazilThe worldwide demand for sustainable bioprocesses is undeniable, as well as for research aimed at the biotechnological exploitation of lignocellulosic materials, especially their hemicellulosic fractions rich in xylose. Various bioproducts can be obtained from these fractions, although some bottlenecks still exist, such as the presence in hemicellulosic hydrolysates of compounds that are toxic for microorganisms, which requires a previous step of detoxification to reduce them to non-inhibitory levels. The present investigation proposes the use of hydrotalcites as a new detoxifying agent for the hemicellulosic hydrolysate of sugarcane straw to produce xylitol by <i>Candida tropicalis</i>, aiming at a greater removal of phenolics and less loss of sugars. The design of these experiments was used for factorial effect analysis in a simultaneous way; the influences of pH and temperature were evaluated, considering the detoxification process at different times for both uncalcined and calcined hydrotalcite adsorbents. While for the calcined hydrotalcite, the temperature was the significant factor, for the uncalcined, there was also an influence of pH and little effect on the factors of yield and productivity. The effectiveness of hydrotalcites as demonstrated in this research, mainly regarding the ability to reduce the content of phenolic compounds in hydrolysates with a low loss of sugar content, followed by fermentability to produce xylitol, is a strong requirement for the proposition of these new adsorbents in investigations of the development of sustainable technologies for obtaining bioproducts in a biorefinery context.https://www.mdpi.com/2311-5637/11/5/243detoxificationhydrolysatehydrotalcitesugarcane strawxylitol
spellingShingle Débora D. V. da Silva
Kelly J. Dussán
Isabela A. L. Costa
Marcus B. S. Forte
Maria G. A. Felipe
Hydrotalcites as a Promising Adsorbent for Hemicellulose Hydrolysate Detoxification in Xylitol Production
Fermentation
detoxification
hydrolysate
hydrotalcite
sugarcane straw
xylitol
title Hydrotalcites as a Promising Adsorbent for Hemicellulose Hydrolysate Detoxification in Xylitol Production
title_full Hydrotalcites as a Promising Adsorbent for Hemicellulose Hydrolysate Detoxification in Xylitol Production
title_fullStr Hydrotalcites as a Promising Adsorbent for Hemicellulose Hydrolysate Detoxification in Xylitol Production
title_full_unstemmed Hydrotalcites as a Promising Adsorbent for Hemicellulose Hydrolysate Detoxification in Xylitol Production
title_short Hydrotalcites as a Promising Adsorbent for Hemicellulose Hydrolysate Detoxification in Xylitol Production
title_sort hydrotalcites as a promising adsorbent for hemicellulose hydrolysate detoxification in xylitol production
topic detoxification
hydrolysate
hydrotalcite
sugarcane straw
xylitol
url https://www.mdpi.com/2311-5637/11/5/243
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AT isabelaalcosta hydrotalcitesasapromisingadsorbentforhemicellulosehydrolysatedetoxificationinxylitolproduction
AT marcusbsforte hydrotalcitesasapromisingadsorbentforhemicellulosehydrolysatedetoxificationinxylitolproduction
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