A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs.
Muscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds...
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Public Library of Science (PLoS)
2019-10-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1008279&type=printable |
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| author | In-Cheol Cho Hee-Bok Park Jin Seop Ahn Sang-Hyun Han Jae-Bong Lee Hyun-Tae Lim Chae-Kyoung Yoo Eun-Ji Jung Dong-Hwan Kim Wu-Sheng Sun Yuliaxis Ramayo-Caldas Sang-Geum Kim Yong-Jun Kang Yoo-Kyung Kim Hyun-Sook Shin Pil-Nam Seong In-Sul Hwang Beom-Young Park Seongsoo Hwang Sung-Soo Lee Youn-Chul Ryu Jun-Heon Lee Moon-Suck Ko Kichoon Lee Göran Andersson Miguel Pérez-Enciso Jeong-Woong Lee |
| author_facet | In-Cheol Cho Hee-Bok Park Jin Seop Ahn Sang-Hyun Han Jae-Bong Lee Hyun-Tae Lim Chae-Kyoung Yoo Eun-Ji Jung Dong-Hwan Kim Wu-Sheng Sun Yuliaxis Ramayo-Caldas Sang-Geum Kim Yong-Jun Kang Yoo-Kyung Kim Hyun-Sook Shin Pil-Nam Seong In-Sul Hwang Beom-Young Park Seongsoo Hwang Sung-Soo Lee Youn-Chul Ryu Jun-Heon Lee Moon-Suck Ko Kichoon Lee Göran Andersson Miguel Pérez-Enciso Jeong-Woong Lee |
| author_sort | In-Cheol Cho |
| collection | DOAJ |
| description | Muscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds and Korean native pigs (KNPs) and a joint linkage-linkage disequilibrium analysis, we identified a 488.1-kb region on porcine chromosome 12 that affects both reddish meat color (a*) and IMF. In this critical region, only the MYH3 gene, encoding myosin heavy chain 3, was found to be preferentially overexpressed in the skeletal muscle of KNPs. Subsequently, MYH3-transgenic mice demonstrated that this gene controls both myofiber-type specification and adipogenesis in skeletal muscle. We discovered a structural variant in the promotor/regulatory region of MYH3 for which Q allele carriers exhibited significantly higher values of a* and IMF than q allele carriers. Furthermore, chromatin immunoprecipitation and cotransfection assays showed that the structural variant in the 5'-flanking region of MYH3 abrogated the binding of the myogenic regulatory factors (MYF5, MYOD, MYOG, and MRF4). The allele distribution of MYH3 among pig populations worldwide indicated that the MYH3 Q allele is of Asian origin and likely predates domestication. In conclusion, we identified a functional regulatory sequence variant in porcine MYH3 that provides novel insights into the genetic basis of the regulation of myofiber type ratios and associated changes in IMF in pigs. The MYH3 variant can play an important role in improving pork quality in current breeding programs. |
| format | Article |
| id | doaj-art-2396d0befb53452ca6ba6897b8ffbb8e |
| institution | OA Journals |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2019-10-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS Genetics |
| spelling | doaj-art-2396d0befb53452ca6ba6897b8ffbb8e2025-08-20T01:52:44ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042019-10-011510e100827910.1371/journal.pgen.1008279A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs.In-Cheol ChoHee-Bok ParkJin Seop AhnSang-Hyun HanJae-Bong LeeHyun-Tae LimChae-Kyoung YooEun-Ji JungDong-Hwan KimWu-Sheng SunYuliaxis Ramayo-CaldasSang-Geum KimYong-Jun KangYoo-Kyung KimHyun-Sook ShinPil-Nam SeongIn-Sul HwangBeom-Young ParkSeongsoo HwangSung-Soo LeeYoun-Chul RyuJun-Heon LeeMoon-Suck KoKichoon LeeGöran AnderssonMiguel Pérez-EncisoJeong-Woong LeeMuscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds and Korean native pigs (KNPs) and a joint linkage-linkage disequilibrium analysis, we identified a 488.1-kb region on porcine chromosome 12 that affects both reddish meat color (a*) and IMF. In this critical region, only the MYH3 gene, encoding myosin heavy chain 3, was found to be preferentially overexpressed in the skeletal muscle of KNPs. Subsequently, MYH3-transgenic mice demonstrated that this gene controls both myofiber-type specification and adipogenesis in skeletal muscle. We discovered a structural variant in the promotor/regulatory region of MYH3 for which Q allele carriers exhibited significantly higher values of a* and IMF than q allele carriers. Furthermore, chromatin immunoprecipitation and cotransfection assays showed that the structural variant in the 5'-flanking region of MYH3 abrogated the binding of the myogenic regulatory factors (MYF5, MYOD, MYOG, and MRF4). The allele distribution of MYH3 among pig populations worldwide indicated that the MYH3 Q allele is of Asian origin and likely predates domestication. In conclusion, we identified a functional regulatory sequence variant in porcine MYH3 that provides novel insights into the genetic basis of the regulation of myofiber type ratios and associated changes in IMF in pigs. The MYH3 variant can play an important role in improving pork quality in current breeding programs.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1008279&type=printable |
| spellingShingle | In-Cheol Cho Hee-Bok Park Jin Seop Ahn Sang-Hyun Han Jae-Bong Lee Hyun-Tae Lim Chae-Kyoung Yoo Eun-Ji Jung Dong-Hwan Kim Wu-Sheng Sun Yuliaxis Ramayo-Caldas Sang-Geum Kim Yong-Jun Kang Yoo-Kyung Kim Hyun-Sook Shin Pil-Nam Seong In-Sul Hwang Beom-Young Park Seongsoo Hwang Sung-Soo Lee Youn-Chul Ryu Jun-Heon Lee Moon-Suck Ko Kichoon Lee Göran Andersson Miguel Pérez-Enciso Jeong-Woong Lee A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs. PLoS Genetics |
| title | A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs. |
| title_full | A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs. |
| title_fullStr | A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs. |
| title_full_unstemmed | A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs. |
| title_short | A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs. |
| title_sort | functional regulatory variant of myh3 influences muscle fiber type composition and intramuscular fat content in pigs |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1008279&type=printable |
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