Evaluation of Creatine Monohydrate Supplementation on the Gastrocnemius Muscle of Mice with Muscular Dystrophy: A Preliminary Study
<b>Background/Objectives:</b> Duchenne muscular dystrophy (DMD) is a genetic disease characterized by a lack of dystrophin caused by mutations in the DMD gene, and some minor cases are due to decreased levels of dystrophin, leading to muscle weakness and motor impairment. Creatine supple...
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2025-01-01
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| author | Victor Augusto Ramos Fernandes Gabriela Pereira dos Santos Amilton Iatecola Daniela Vieira Buchaim Ionaly Judith Faria Garcia Carlos Henrique Bertoni Reis Lívia Maluf Menegazzo Bueno Bruna Trazzi Pagani Rogerio Leone Buchaim Marcelo Rodrigues da Cunha |
| author_facet | Victor Augusto Ramos Fernandes Gabriela Pereira dos Santos Amilton Iatecola Daniela Vieira Buchaim Ionaly Judith Faria Garcia Carlos Henrique Bertoni Reis Lívia Maluf Menegazzo Bueno Bruna Trazzi Pagani Rogerio Leone Buchaim Marcelo Rodrigues da Cunha |
| author_sort | Victor Augusto Ramos Fernandes |
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| description | <b>Background/Objectives:</b> Duchenne muscular dystrophy (DMD) is a genetic disease characterized by a lack of dystrophin caused by mutations in the DMD gene, and some minor cases are due to decreased levels of dystrophin, leading to muscle weakness and motor impairment. Creatine supplementation has demonstrated several benefits for the muscle, such as increased strength, enhanced tissue repair, and improved ATP resynthesis. This preliminary study aimed to investigate the effects of creatine on the gastrocnemius muscle in dystrophy muscle (MDX) and healthy C57BL/10 mice. <b>Methods:</b> Twenty MDX and C57Bl/10 mice were organized into groups and supplemented or not with creatine in a dosage of 0.3 mg for 8 weeks. Gastrocnemius tissue was analyzed using histomorphology and histomorphometric techniques. <b>Results:</b> The results demonstrated potential anti-inflammatory effects of creatine, with less observation of inflammatory infiltrates, the preservation of intramuscular glycogen, and reduction in tissue fibrosis in supplemented animals. <b>Conclusions:</b> These findings suggest that creatine may enhance tissue function and slow the progression of DMD. However, further research, with more analysis, is needed to elucidate molecular mechanisms underlying creatine’s effects on reducing mononuclear leukocytes and its role in mitigating tissue fibrosis. |
| format | Article |
| id | doaj-art-239593b647734e05826f0d6cb206bb29 |
| institution | OA Journals |
| issn | 1873-149X |
| language | English |
| publishDate | 2025-01-01 |
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| series | Pathophysiology |
| spelling | doaj-art-239593b647734e05826f0d6cb206bb292025-08-20T01:48:53ZengMDPI AGPathophysiology1873-149X2025-01-01321210.3390/pathophysiology32010002Evaluation of Creatine Monohydrate Supplementation on the Gastrocnemius Muscle of Mice with Muscular Dystrophy: A Preliminary StudyVictor Augusto Ramos Fernandes0Gabriela Pereira dos Santos1Amilton Iatecola2Daniela Vieira Buchaim3Ionaly Judith Faria Garcia4Carlos Henrique Bertoni Reis5Lívia Maluf Menegazzo Bueno6Bruna Trazzi Pagani7Rogerio Leone Buchaim8Marcelo Rodrigues da Cunha9Postgraduate Program in Health Sciences, Faculty of Medicine of Jundiaí (FMJ), Jundiaí 13202-550, BrazilNeurobiology Study Group, Nossa Senhora do Patrocínio University Center (CEUNSP), Itu 13300-200, BrazilNeurobiology Study Group, Nossa Senhora do Patrocínio University Center (CEUNSP), Itu 13300-200, BrazilGraduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of Sao Paulo (FMVZ/USP), Sao Paulo 05508-270, BrazilMedical and Dentistry School, University Center of Adamantina (FAI), Adamantina 17800-000, BrazilBeneficent Hospital (HBU), University of Marilia (UNIMAR), Marilia 17525-160, BrazilMedical and Dentistry School, University Center of Adamantina (FAI), Adamantina 17800-000, BrazilDentistry School, University of Marilia (UNIMAR), Marilia 17525-902, BrazilGraduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of Sao Paulo (FMVZ/USP), Sao Paulo 05508-270, BrazilPostgraduate Program in Health Sciences, Faculty of Medicine of Jundiaí (FMJ), Jundiaí 13202-550, Brazil<b>Background/Objectives:</b> Duchenne muscular dystrophy (DMD) is a genetic disease characterized by a lack of dystrophin caused by mutations in the DMD gene, and some minor cases are due to decreased levels of dystrophin, leading to muscle weakness and motor impairment. Creatine supplementation has demonstrated several benefits for the muscle, such as increased strength, enhanced tissue repair, and improved ATP resynthesis. This preliminary study aimed to investigate the effects of creatine on the gastrocnemius muscle in dystrophy muscle (MDX) and healthy C57BL/10 mice. <b>Methods:</b> Twenty MDX and C57Bl/10 mice were organized into groups and supplemented or not with creatine in a dosage of 0.3 mg for 8 weeks. Gastrocnemius tissue was analyzed using histomorphology and histomorphometric techniques. <b>Results:</b> The results demonstrated potential anti-inflammatory effects of creatine, with less observation of inflammatory infiltrates, the preservation of intramuscular glycogen, and reduction in tissue fibrosis in supplemented animals. <b>Conclusions:</b> These findings suggest that creatine may enhance tissue function and slow the progression of DMD. However, further research, with more analysis, is needed to elucidate molecular mechanisms underlying creatine’s effects on reducing mononuclear leukocytes and its role in mitigating tissue fibrosis.https://www.mdpi.com/1873-149X/32/1/2Duchenne muscular dystrophycreatine supplementationcreatine monohydrateskeletal striated musclemyopathy |
| spellingShingle | Victor Augusto Ramos Fernandes Gabriela Pereira dos Santos Amilton Iatecola Daniela Vieira Buchaim Ionaly Judith Faria Garcia Carlos Henrique Bertoni Reis Lívia Maluf Menegazzo Bueno Bruna Trazzi Pagani Rogerio Leone Buchaim Marcelo Rodrigues da Cunha Evaluation of Creatine Monohydrate Supplementation on the Gastrocnemius Muscle of Mice with Muscular Dystrophy: A Preliminary Study Pathophysiology Duchenne muscular dystrophy creatine supplementation creatine monohydrate skeletal striated muscle myopathy |
| title | Evaluation of Creatine Monohydrate Supplementation on the Gastrocnemius Muscle of Mice with Muscular Dystrophy: A Preliminary Study |
| title_full | Evaluation of Creatine Monohydrate Supplementation on the Gastrocnemius Muscle of Mice with Muscular Dystrophy: A Preliminary Study |
| title_fullStr | Evaluation of Creatine Monohydrate Supplementation on the Gastrocnemius Muscle of Mice with Muscular Dystrophy: A Preliminary Study |
| title_full_unstemmed | Evaluation of Creatine Monohydrate Supplementation on the Gastrocnemius Muscle of Mice with Muscular Dystrophy: A Preliminary Study |
| title_short | Evaluation of Creatine Monohydrate Supplementation on the Gastrocnemius Muscle of Mice with Muscular Dystrophy: A Preliminary Study |
| title_sort | evaluation of creatine monohydrate supplementation on the gastrocnemius muscle of mice with muscular dystrophy a preliminary study |
| topic | Duchenne muscular dystrophy creatine supplementation creatine monohydrate skeletal striated muscle myopathy |
| url | https://www.mdpi.com/1873-149X/32/1/2 |
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