Multiplex polymerase chain reaction (PCR) with Nanopore sequencing for sequence-based detection of four tilapia pathogens

Multiplex polymerase chain reaction (PCR) with Nanopore sequencing for sequence-based detection of four tilapia pathogens

Background Tilapia aquaculture faces significant threats posed by four prominent pathogens: tilapia lake virus (TiLV), infectious spleen and kidney necrosis virus (ISKNV), Francisella orientalis, and Streptococcus agalactiae. Currently, employed molecular diagnostic methods for these pathogens rely...

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Bibliographic Details
Main Authors: Jérôme Delamare-Deboutteville, Watcharachai Meemetta, Khaettareeya Pimsannil, Han Ming Gan, Laura Khor, Mohan Chadag, Ha Thanh Dong, Saengchan Senapin
Format: Article
Language:English
Published: PeerJ Inc. 2025-05-01
Series:PeerJ
Subjects:
Nanopore
Multiplex PCR
Tilapia
TiLV
ISKNV
Francisella orientalis
Online Access:https://peerj.com/articles/19425.pdf
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