Influence of the Angle of Periodontal Intrabony Defects on Blood Clots: A Confocal Microscopy Study
Intrabony defects can be the result of parodontitis. In this study we aimed to examine how clot stability is affected by different infrabony defect angles and superficial treatments in regenerative surgery. Methods: Thirty single-root extracted teeth were cut to obtain a section from each tooth. Thi...
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MDPI AG
2024-11-01
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| author | Lorenzo Bevilacqua Sofia Patatti Andrea Macrì Veronica Del Lupo Davide Porrelli Michele Maglione |
| author_facet | Lorenzo Bevilacqua Sofia Patatti Andrea Macrì Veronica Del Lupo Davide Porrelli Michele Maglione |
| author_sort | Lorenzo Bevilacqua |
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| description | Intrabony defects can be the result of parodontitis. In this study we aimed to examine how clot stability is affected by different infrabony defect angles and superficial treatments in regenerative surgery. Methods: Thirty single-root extracted teeth were cut to obtain a section from each tooth. This section was placed in an artificial model containing an infrabony defect with three walls in order to achieve 10 models at angles of 25°, 37°, and 50°. Five root samples for every angle were not subjected any treatment (NT) and five were subjected to root conditioning with a neutral pH solution of ethylenediaminetetraacetic acid (EDTA) at 24%, applied for 2 min. Venous blood was put into the artificial models containing the root sections, and these were placed in an incubator at a constant temperature of 37 °C for 2 h. Samples were analyzed by laser confocal microscopy. Results: All samples exhibited signs of retraction. The EDTA group exhibited a plateau trend for infrabony defects, while in the NT group, there was a statistically significant difference between clot retraction and the increase in defect amplitude. Conclusion: The greater the angle of the infraosseous defect, the higher the rate of clot retraction. This showed that EDTA could reduce the influence of the angle of the infraosseous defect. |
| format | Article |
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| issn | 2076-3417 |
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| publishDate | 2024-11-01 |
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| spelling | doaj-art-2386c706e95442738aed8ca84a3d12ca2025-08-20T02:07:57ZengMDPI AGApplied Sciences2076-34172024-11-0114221030610.3390/app142210306Influence of the Angle of Periodontal Intrabony Defects on Blood Clots: A Confocal Microscopy StudyLorenzo Bevilacqua0Sofia Patatti1Andrea Macrì2Veronica Del Lupo3Davide Porrelli4Michele Maglione5Department of Medical, Surgical and Health Sciences, University of Trieste, Piazza dell’Ospitale 1, 34125 Trieste, ItalyIndependent Researcher, Via Sopra Fontana 1, Tolmezzo, 33080 Udine, ItalyPrivate Practice, Via San Lazzaro 3, 34121 Trieste, ItalyPrivate Practice, Via Jacopo Marinoni 16, 33100 Udine, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Medical, Surgical and Health Sciences, University of Trieste, Piazza dell’Ospitale 1, 34125 Trieste, ItalyIntrabony defects can be the result of parodontitis. In this study we aimed to examine how clot stability is affected by different infrabony defect angles and superficial treatments in regenerative surgery. Methods: Thirty single-root extracted teeth were cut to obtain a section from each tooth. This section was placed in an artificial model containing an infrabony defect with three walls in order to achieve 10 models at angles of 25°, 37°, and 50°. Five root samples for every angle were not subjected any treatment (NT) and five were subjected to root conditioning with a neutral pH solution of ethylenediaminetetraacetic acid (EDTA) at 24%, applied for 2 min. Venous blood was put into the artificial models containing the root sections, and these were placed in an incubator at a constant temperature of 37 °C for 2 h. Samples were analyzed by laser confocal microscopy. Results: All samples exhibited signs of retraction. The EDTA group exhibited a plateau trend for infrabony defects, while in the NT group, there was a statistically significant difference between clot retraction and the increase in defect amplitude. Conclusion: The greater the angle of the infraosseous defect, the higher the rate of clot retraction. This showed that EDTA could reduce the influence of the angle of the infraosseous defect.https://www.mdpi.com/2076-3417/14/22/10306intrabony defectblood clotEDTAconfocal microscopy |
| spellingShingle | Lorenzo Bevilacqua Sofia Patatti Andrea Macrì Veronica Del Lupo Davide Porrelli Michele Maglione Influence of the Angle of Periodontal Intrabony Defects on Blood Clots: A Confocal Microscopy Study Applied Sciences intrabony defect blood clot EDTA confocal microscopy |
| title | Influence of the Angle of Periodontal Intrabony Defects on Blood Clots: A Confocal Microscopy Study |
| title_full | Influence of the Angle of Periodontal Intrabony Defects on Blood Clots: A Confocal Microscopy Study |
| title_fullStr | Influence of the Angle of Periodontal Intrabony Defects on Blood Clots: A Confocal Microscopy Study |
| title_full_unstemmed | Influence of the Angle of Periodontal Intrabony Defects on Blood Clots: A Confocal Microscopy Study |
| title_short | Influence of the Angle of Periodontal Intrabony Defects on Blood Clots: A Confocal Microscopy Study |
| title_sort | influence of the angle of periodontal intrabony defects on blood clots a confocal microscopy study |
| topic | intrabony defect blood clot EDTA confocal microscopy |
| url | https://www.mdpi.com/2076-3417/14/22/10306 |
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