The phytochemical and antibacterial activity of ethanolic extract of Laportea decumana and Laportea aestuans
Plants have been an important medicinal resource for centuries, offering a rich source of bioactive compounds used in traditional and modern medicine. This study aims to analyze the comparison between Laportea decumana and Laportea aestuans, focusing on the two main aspects, phytochemical screening...
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| Format: | Article |
| Language: | English |
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Universitas Ahmad Dahlan
2025-03-01
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| Series: | Pharmaciana |
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| Online Access: | https://journal.uad.ac.id/index.php/PHARMACIANA/article/view/30099 |
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| author | Esty Vicariat Siahaya Elizabeth Betty Elok Kristiani Sri Kasmiyati |
| author_facet | Esty Vicariat Siahaya Elizabeth Betty Elok Kristiani Sri Kasmiyati |
| author_sort | Esty Vicariat Siahaya |
| collection | DOAJ |
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Plants have been an important medicinal resource for centuries, offering a rich source of bioactive compounds used in traditional and modern medicine. This study aims to analyze the comparison between Laportea decumana and Laportea aestuans, focusing on the two main aspects, phytochemical screening and antibacterial activity. The extraction of the maceration process uses ethanol 96% as the solvent at room temperature for 72 hours. The qualitative phytochemical analysis uses HCl and FeCl3 0.1% reagents to determine flavonoids and phenolic compounds, respectively. The quantitative analysis of total flavonoid content is measured using colorimetric AlCl₃ method, while the determination of phenolic compounds is performed by the Folin-Ciocalteu. The diffusion method is used to determine the antibacterial activity. The 96% ethanol extract of L. decumana leaves has higher flavonoids (69.4 mg GAE/g extract) than phenolics (55.1 mg QE/g extract). However, the L. aestuans has higher phenolic content (55.3 mg QE/g extract) than flavonoids (35.3 mg GAE/g extract). The antibacterial test using agar diffusion method shows that both extracts inhibited the growth of pure strains of E. coli ATCC and S. aureus ATCC, with different potencies. The L. decumana extract showed 1.5 times stronger activity than the L. aestuans extract on the growth inhibitory activity against E. coli bacteria. The results show that the relationship between the antibacterial activity and the phytochemical profile against E. coli is strong but weak against S. aureus.
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| format | Article |
| id | doaj-art-237eac9f7e2a4ef787d1c9d68f45c84b |
| institution | Kabale University |
| issn | 2088-4559 2477-0256 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Universitas Ahmad Dahlan |
| record_format | Article |
| series | Pharmaciana |
| spelling | doaj-art-237eac9f7e2a4ef787d1c9d68f45c84b2025-08-20T03:40:00ZengUniversitas Ahmad DahlanPharmaciana2088-45592477-02562025-03-0115110.12928/pharmaciana.v15i1.30099The phytochemical and antibacterial activity of ethanolic extract of Laportea decumana and Laportea aestuansEsty Vicariat Siahaya0Elizabeth Betty Elok Kristiani1Sri Kasmiyati2Faculty of Biologi, Satya Wacana Christian UniversityFaculty of BiologySatya Wacana Christian UniversityFaculty of BiologySatya Wacana Christian University Plants have been an important medicinal resource for centuries, offering a rich source of bioactive compounds used in traditional and modern medicine. This study aims to analyze the comparison between Laportea decumana and Laportea aestuans, focusing on the two main aspects, phytochemical screening and antibacterial activity. The extraction of the maceration process uses ethanol 96% as the solvent at room temperature for 72 hours. The qualitative phytochemical analysis uses HCl and FeCl3 0.1% reagents to determine flavonoids and phenolic compounds, respectively. The quantitative analysis of total flavonoid content is measured using colorimetric AlCl₃ method, while the determination of phenolic compounds is performed by the Folin-Ciocalteu. The diffusion method is used to determine the antibacterial activity. The 96% ethanol extract of L. decumana leaves has higher flavonoids (69.4 mg GAE/g extract) than phenolics (55.1 mg QE/g extract). However, the L. aestuans has higher phenolic content (55.3 mg QE/g extract) than flavonoids (35.3 mg GAE/g extract). The antibacterial test using agar diffusion method shows that both extracts inhibited the growth of pure strains of E. coli ATCC and S. aureus ATCC, with different potencies. The L. decumana extract showed 1.5 times stronger activity than the L. aestuans extract on the growth inhibitory activity against E. coli bacteria. The results show that the relationship between the antibacterial activity and the phytochemical profile against E. coli is strong but weak against S. aureus. https://journal.uad.ac.id/index.php/PHARMACIANA/article/view/30099Phytochemistry, antibacterial, Laportea decumana, Laportea aestuans, |
| spellingShingle | Esty Vicariat Siahaya Elizabeth Betty Elok Kristiani Sri Kasmiyati The phytochemical and antibacterial activity of ethanolic extract of Laportea decumana and Laportea aestuans Pharmaciana Phytochemistry, antibacterial, Laportea decumana, Laportea aestuans, |
| title | The phytochemical and antibacterial activity of ethanolic extract of Laportea decumana and Laportea aestuans |
| title_full | The phytochemical and antibacterial activity of ethanolic extract of Laportea decumana and Laportea aestuans |
| title_fullStr | The phytochemical and antibacterial activity of ethanolic extract of Laportea decumana and Laportea aestuans |
| title_full_unstemmed | The phytochemical and antibacterial activity of ethanolic extract of Laportea decumana and Laportea aestuans |
| title_short | The phytochemical and antibacterial activity of ethanolic extract of Laportea decumana and Laportea aestuans |
| title_sort | phytochemical and antibacterial activity of ethanolic extract of laportea decumana and laportea aestuans |
| topic | Phytochemistry, antibacterial, Laportea decumana, Laportea aestuans, |
| url | https://journal.uad.ac.id/index.php/PHARMACIANA/article/view/30099 |
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