Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans
The age-associated alterations in microbiomes vary across populations due to the influence of genetics and lifestyles. To the best of our knowledge, the microbial changes associated with aging have not yet been investigated in Singapore adults. We conducted shotgun metagenomic sequencing of fecal an...
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Taylor & Francis Group
2022-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2022.2070392 |
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| author | Liwei Chen Tingting Zheng Yifan Yang Prem Prashant Chaudhary Jean Pui Yi Teh Bobby K. Cheon Daniela Moses Stephan C. Schuster Joergen Schlundt Jun Li Patricia L. Conway |
| author_facet | Liwei Chen Tingting Zheng Yifan Yang Prem Prashant Chaudhary Jean Pui Yi Teh Bobby K. Cheon Daniela Moses Stephan C. Schuster Joergen Schlundt Jun Li Patricia L. Conway |
| author_sort | Liwei Chen |
| collection | DOAJ |
| description | The age-associated alterations in microbiomes vary across populations due to the influence of genetics and lifestyles. To the best of our knowledge, the microbial changes associated with aging have not yet been investigated in Singapore adults. We conducted shotgun metagenomic sequencing of fecal and saliva samples, as well as fecal metabolomics to characterize the gut and oral microbial communities of 62 healthy adult male Singaporeans, including 32 young subjects (age, 23.1 ± 1.4 years) and 30 elderly subjects (age, 69.0 ± 3.5 years). We identified 8 gut and 13 oral species that were differentially abundant in elderly compared to young subjects. By combining the gut and oral microbiomes, 25 age-associated oral-gut species connections were identified. Moreover, oral bacteria Acidaminococcus intestine and Flavonifractor plautii were less prevalent/abundant in elderly gut samples than in young gut samples, whereas Collinsella aerofaciens and Roseburia hominis showed the opposite trends. These results indicate the varied gut-oral communications with aging. Subsequently, we expanded the association studies on microbiome, metabolome and host phenotypic parameters. In particular, Eubacterium eligens increased in elderly compared to young subjects, and was positively correlated with triglycerides, which implies that the potential role of E. eligens in lipid metabolism is altered during the aging process. Our results demonstrated aging-associated changes in the gut and oral microbiomes, as well as the connections between metabolites and host-microbe interactions, thereby deepening the understanding of alterations in the human microbiome during the aging process in a Singapore population. |
| format | Article |
| id | doaj-art-2375e3fb19e64dcbbc9a92ffe175f37a |
| institution | DOAJ |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-2375e3fb19e64dcbbc9a92ffe175f37a2025-08-20T03:05:34ZengTaylor & Francis GroupGut Microbes1949-09761949-09842022-12-0114110.1080/19490976.2022.2070392Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in SingaporeansLiwei Chen0Tingting Zheng1Yifan Yang2Prem Prashant Chaudhary3Jean Pui Yi Teh4Bobby K. Cheon5Daniela Moses6Stephan C. Schuster7Joergen Schlundt8Jun Li9Patricia L. Conway10School of Chemical and Biomedical Engineering, College of Engineering, Nanyang Technological University, SingaporeDepartment of Infectious Diseases and Public Health, The Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong, ChinaOffice of Education Research, and Physical Education and Sports Science, National Institute of Education, Nanyang Technological University, SingaporeSchool of Chemical and Biomedical Engineering, College of Engineering, Nanyang Technological University, SingaporeSchool of Chemical and Biomedical Engineering, College of Engineering, Nanyang Technological University, SingaporeSchool of Social Sciences, Nanyang Technological University, SingaporeSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, SingaporeSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, SingaporeSchool of Chemical and Biomedical Engineering, College of Engineering, Nanyang Technological University, SingaporeDepartment of Infectious Diseases and Public Health, The Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong, ChinaSchool of Chemical and Biomedical Engineering, College of Engineering, Nanyang Technological University, SingaporeThe age-associated alterations in microbiomes vary across populations due to the influence of genetics and lifestyles. To the best of our knowledge, the microbial changes associated with aging have not yet been investigated in Singapore adults. We conducted shotgun metagenomic sequencing of fecal and saliva samples, as well as fecal metabolomics to characterize the gut and oral microbial communities of 62 healthy adult male Singaporeans, including 32 young subjects (age, 23.1 ± 1.4 years) and 30 elderly subjects (age, 69.0 ± 3.5 years). We identified 8 gut and 13 oral species that were differentially abundant in elderly compared to young subjects. By combining the gut and oral microbiomes, 25 age-associated oral-gut species connections were identified. Moreover, oral bacteria Acidaminococcus intestine and Flavonifractor plautii were less prevalent/abundant in elderly gut samples than in young gut samples, whereas Collinsella aerofaciens and Roseburia hominis showed the opposite trends. These results indicate the varied gut-oral communications with aging. Subsequently, we expanded the association studies on microbiome, metabolome and host phenotypic parameters. In particular, Eubacterium eligens increased in elderly compared to young subjects, and was positively correlated with triglycerides, which implies that the potential role of E. eligens in lipid metabolism is altered during the aging process. Our results demonstrated aging-associated changes in the gut and oral microbiomes, as well as the connections between metabolites and host-microbe interactions, thereby deepening the understanding of alterations in the human microbiome during the aging process in a Singapore population.https://www.tandfonline.com/doi/10.1080/19490976.2022.2070392Microbiomefecal metabolomemulti-omicsintegrative analysisaging |
| spellingShingle | Liwei Chen Tingting Zheng Yifan Yang Prem Prashant Chaudhary Jean Pui Yi Teh Bobby K. Cheon Daniela Moses Stephan C. Schuster Joergen Schlundt Jun Li Patricia L. Conway Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans Gut Microbes Microbiome fecal metabolome multi-omics integrative analysis aging |
| title | Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans |
| title_full | Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans |
| title_fullStr | Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans |
| title_full_unstemmed | Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans |
| title_short | Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans |
| title_sort | integrative multiomics analysis reveals host microbe metabolite interplays associated with the aging process in singaporeans |
| topic | Microbiome fecal metabolome multi-omics integrative analysis aging |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2022.2070392 |
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