Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA Profiling
Small extracellular vesicles (sEVs) derived from mesenchymal stem cells have emerged as promising therapeutic agents in regenerative dermatology. This study evaluated the safety and efficacy of Bio-Pulsed avian mesenchymal stem cell-derived sEVs (AMSC-sEVs), topically applied for hair follicle stimu...
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MDPI AG
2025-07-01
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| Series: | Current Issues in Molecular Biology |
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| author | Ju-Sheng Shieh Yu-Tang Chin Tsu-Te Yeh Jiong Jiong Guo Fung-Wei Chang Hui-Rong Cheng Hung-Han Hsu Wei-Lun Huang Han-Hsiang Huang Ya-Yu Hsieh Chien-Ping Chiang Shih-Ching Wang |
| author_facet | Ju-Sheng Shieh Yu-Tang Chin Tsu-Te Yeh Jiong Jiong Guo Fung-Wei Chang Hui-Rong Cheng Hung-Han Hsu Wei-Lun Huang Han-Hsiang Huang Ya-Yu Hsieh Chien-Ping Chiang Shih-Ching Wang |
| author_sort | Ju-Sheng Shieh |
| collection | DOAJ |
| description | Small extracellular vesicles (sEVs) derived from mesenchymal stem cells have emerged as promising therapeutic agents in regenerative dermatology. This study evaluated the safety and efficacy of Bio-Pulsed avian mesenchymal stem cell-derived sEVs (AMSC-sEVs), topically applied for hair follicle stimulation and skin rejuvenation. Two prospective, single-arm clinical trials were conducted: one involving 30 participants using a hair ampoule over 60 days, and the other involving 30 participants applying a facial essence for 28 days. Objective measurements demonstrated significant improvements in the anagen/telogen hair ratio, reduced shedding, increased collagen density, and reduced wrinkle depth and pigmentation. Small RNA sequencing and qPCR profiling confirmed that Bio-Pulsed AMSC-sEVs were enriched with regenerative microRNAs, such as miR-21-5p and miR-199a-5p, associated with anti-inflammatory and anti-aging effects. No adverse events were reported. These findings suggest that Bio-Pulsed AMSC-sEVs may offer a safe, non-invasive, and cell-free approach to enhance skin and hair regeneration in human subjects. |
| format | Article |
| id | doaj-art-2356c8b3fd184afb86207048e855ab79 |
| institution | DOAJ |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-2356c8b3fd184afb86207048e855ab792025-08-20T02:45:34ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-07-0147753910.3390/cimb47070539Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA ProfilingJu-Sheng Shieh0Yu-Tang Chin1Tsu-Te Yeh2Jiong Jiong Guo3Fung-Wei Chang4Hui-Rong Cheng5Hung-Han Hsu6Wei-Lun Huang7Han-Hsiang Huang8Ya-Yu Hsieh9Chien-Ping Chiang10Shih-Ching Wang11Department of Periodontology, School of Dentistry, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, TaiwanDepartment of Periodontology, School of Dentistry, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, TaiwanDepartment of Orthopaedic Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, TaiwanDepartment of Orthopedics and Sports Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, ChinaDepartment of Obstetrics and Gynecology, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, TaiwanDepartment of Periodontology, School of Dentistry, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, TaiwanDepartment of Orthopedics and Sports Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, ChinaDepartment of Research and Development, Ascension Medical Biotechnology Co., Ltd., Taipei 114731, TaiwanDepartment of Research and Development, Ascension Medical Biotechnology Co., Ltd., Taipei 114731, TaiwanDepartment and Graduate Institute of Pharmacology, College of Medicine, National Taiwan University, Taipei 110225, TaiwanDepartment of Dermatology, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, TaiwanDepartment of Dermatology, Saint Paul’s Hospital, Taoyuan 330049, TaiwanSmall extracellular vesicles (sEVs) derived from mesenchymal stem cells have emerged as promising therapeutic agents in regenerative dermatology. This study evaluated the safety and efficacy of Bio-Pulsed avian mesenchymal stem cell-derived sEVs (AMSC-sEVs), topically applied for hair follicle stimulation and skin rejuvenation. Two prospective, single-arm clinical trials were conducted: one involving 30 participants using a hair ampoule over 60 days, and the other involving 30 participants applying a facial essence for 28 days. Objective measurements demonstrated significant improvements in the anagen/telogen hair ratio, reduced shedding, increased collagen density, and reduced wrinkle depth and pigmentation. Small RNA sequencing and qPCR profiling confirmed that Bio-Pulsed AMSC-sEVs were enriched with regenerative microRNAs, such as miR-21-5p and miR-199a-5p, associated with anti-inflammatory and anti-aging effects. No adverse events were reported. These findings suggest that Bio-Pulsed AMSC-sEVs may offer a safe, non-invasive, and cell-free approach to enhance skin and hair regeneration in human subjects.https://www.mdpi.com/1467-3045/47/7/539extracellular vesiclesBio-Pulsed primingavian mesenchymal stem cellsregenerative dermatologyhair follicle activationskin rejuvenation |
| spellingShingle | Ju-Sheng Shieh Yu-Tang Chin Tsu-Te Yeh Jiong Jiong Guo Fung-Wei Chang Hui-Rong Cheng Hung-Han Hsu Wei-Lun Huang Han-Hsiang Huang Ya-Yu Hsieh Chien-Ping Chiang Shih-Ching Wang Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA Profiling Current Issues in Molecular Biology extracellular vesicles Bio-Pulsed priming avian mesenchymal stem cells regenerative dermatology hair follicle activation skin rejuvenation |
| title | Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA Profiling |
| title_full | Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA Profiling |
| title_fullStr | Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA Profiling |
| title_full_unstemmed | Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA Profiling |
| title_short | Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA Profiling |
| title_sort | topical application of bio pulsed avian msc derived extracellular vesicles enhances hair regrowth and skin rejuvenation evidence from clinical evaluation and mirna profiling |
| topic | extracellular vesicles Bio-Pulsed priming avian mesenchymal stem cells regenerative dermatology hair follicle activation skin rejuvenation |
| url | https://www.mdpi.com/1467-3045/47/7/539 |
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