The Influence of Genotypes and Culture Medium on In Vitro Proliferation in Heuchera spp.
The genus Heuchera includes perennials prized worldwide in gardens for their ornamental foliage and flowers. This study aimed to assess the in vitro proliferation efficiency of two Heuchera genotypes under different culture media conditions of two Heuchera genotypes, Heuchera villosa 'Silver Gu...
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AcademicPres
2025-05-01
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| Series: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture |
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| Online Access: | https://journals.usamvcluj.ro/index.php/horticulture/article/view/15147 |
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| author | Paula OROS Corina CĂTANĂ Cristian Cătălin CIOBANU |
| author_facet | Paula OROS Corina CĂTANĂ Cristian Cătălin CIOBANU |
| author_sort | Paula OROS |
| collection | DOAJ |
| description | The genus Heuchera includes perennials prized worldwide in gardens for their ornamental foliage and flowers. This study aimed to assess the in vitro proliferation efficiency of two Heuchera genotypes under different culture media conditions of two Heuchera genotypes, Heuchera villosa 'Silver Gumdrop' (SG) and Heuchera x hybrida 'Timeless Orange' (TO) by testing different culture media. The in vitro culture was initiated on two culture medium compositions: Murashige & Skoog, 1962 (MS) and Murashige & Skoog, 1962 with Gamborg B5 vitamins (MSB5). The regeneration rate of explants was evaluated. In the multiplication stage, in addition to the two culture medium compositions, the influence of cytokinin benzylaminopurine (BAP) on the plantlets proliferation was tested. Among the two culture media used at initiation, MSB5 resulted in regeneration rates of 96.67% for SG and 93.33% for TO. Similar situation was observed for shoot proliferation. By using MSB5 the highest shoot proliferation was obtained for both genotypes: 5.92 shoots for SG and 4.13 for TO respectively. However, supplementing the culture medium with BAP resulted in high vitrification rates. The results demonstrate the protocol's efficiency in producing large quantities of healthy, uniform plants within a reduced cultivation period. Both culture media led to high production efficiencies, which recommends multiplication in Heuchera spp. by micropropagation. |
| format | Article |
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| institution | DOAJ |
| issn | 1843-5254 1843-5394 |
| language | English |
| publishDate | 2025-05-01 |
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| series | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture |
| spelling | doaj-art-234f8a30a56b499ebf088545dc089cf72025-08-20T03:12:12ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture1843-52541843-53942025-05-01821556210.15835/buasvmcn-hort:2024.003315147The Influence of Genotypes and Culture Medium on In Vitro Proliferation in Heuchera spp.Paula OROS0Corina CĂTANĂ1Cristian Cătălin CIOBANU2Faculty of Horticulture and Business in Rural Development, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăștur St., 400374, Cluj-NapocaFaculty of Horticulture and Business in Rural Development, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăștur St., 400374, Cluj-NapocaFaculty of Horticulture and Business in Rural Development, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăștur St., 400374, Cluj-NapocaThe genus Heuchera includes perennials prized worldwide in gardens for their ornamental foliage and flowers. This study aimed to assess the in vitro proliferation efficiency of two Heuchera genotypes under different culture media conditions of two Heuchera genotypes, Heuchera villosa 'Silver Gumdrop' (SG) and Heuchera x hybrida 'Timeless Orange' (TO) by testing different culture media. The in vitro culture was initiated on two culture medium compositions: Murashige & Skoog, 1962 (MS) and Murashige & Skoog, 1962 with Gamborg B5 vitamins (MSB5). The regeneration rate of explants was evaluated. In the multiplication stage, in addition to the two culture medium compositions, the influence of cytokinin benzylaminopurine (BAP) on the plantlets proliferation was tested. Among the two culture media used at initiation, MSB5 resulted in regeneration rates of 96.67% for SG and 93.33% for TO. Similar situation was observed for shoot proliferation. By using MSB5 the highest shoot proliferation was obtained for both genotypes: 5.92 shoots for SG and 4.13 for TO respectively. However, supplementing the culture medium with BAP resulted in high vitrification rates. The results demonstrate the protocol's efficiency in producing large quantities of healthy, uniform plants within a reduced cultivation period. Both culture media led to high production efficiencies, which recommends multiplication in Heuchera spp. by micropropagation.https://journals.usamvcluj.ro/index.php/horticulture/article/view/15147coral bells; ornamental plants micropropagation; murashige & skoog; gamborg b5 vitamins; organogenesis. |
| spellingShingle | Paula OROS Corina CĂTANĂ Cristian Cătălin CIOBANU The Influence of Genotypes and Culture Medium on In Vitro Proliferation in Heuchera spp. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture coral bells; ornamental plants micropropagation; murashige & skoog; gamborg b5 vitamins; organogenesis. |
| title | The Influence of Genotypes and Culture Medium on In Vitro Proliferation in Heuchera spp. |
| title_full | The Influence of Genotypes and Culture Medium on In Vitro Proliferation in Heuchera spp. |
| title_fullStr | The Influence of Genotypes and Culture Medium on In Vitro Proliferation in Heuchera spp. |
| title_full_unstemmed | The Influence of Genotypes and Culture Medium on In Vitro Proliferation in Heuchera spp. |
| title_short | The Influence of Genotypes and Culture Medium on In Vitro Proliferation in Heuchera spp. |
| title_sort | influence of genotypes and culture medium on in vitro proliferation in heuchera spp |
| topic | coral bells; ornamental plants micropropagation; murashige & skoog; gamborg b5 vitamins; organogenesis. |
| url | https://journals.usamvcluj.ro/index.php/horticulture/article/view/15147 |
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