Fecal metabolomics reveals the positive effect of ethanol extract of propolis on T2DM mice
A large number of studies have shown that propolis has positive effects in the treatment of type 2 diabetes mellitus (T2DM). However, there are have only been a few reports that are based on an ultra-high performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) a...
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Tsinghua University Press
2023-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001410 |
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| author | Chunmei Wang Huiting Zhao Kai Xu Yali Du Jinjia Liu Jinfei Wang Yusuo Jiang |
| author_facet | Chunmei Wang Huiting Zhao Kai Xu Yali Du Jinjia Liu Jinfei Wang Yusuo Jiang |
| author_sort | Chunmei Wang |
| collection | DOAJ |
| description | A large number of studies have shown that propolis has positive effects in the treatment of type 2 diabetes mellitus (T2DM). However, there are have only been a few reports that are based on an ultra-high performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) analysis of the fecal metabolomics of ethanol extract of propolis (EEP) in the treatment of T2DM. The present investigation was designed to screen potential biomarkers of T2DM by the metabonomic method and to explain the possible anti-diabetes mechanism of EEP according to the changes in the biomarkers. The results showed that EEP improved the body weight (BW) of T2DM mice, lowered blood sugar levels, and significantly restored blood biochemical indicators related to T2DM, such as fasting insulin (FINS), homeostasis model assessment of insulin resistance (HOMA-IR), aspartate transaminase (AST), and alanine aminotransferase (ALT). Liver pathology showed that EEP reversed liver damage caused by T2DM. Metabolomics data identified 27 potential biomarkers in fecal samples. EEP effectively regulated the dysfunction in the metabolic pathways of glycerophospholipids, sphingolipids, riboflavins, and sterol lipids caused by T2DM. In summary, our research results revealed positive effects of EEP in the treatment of T2DM and provided potential candidate markers for further research and in the clinical treatment of T2DM. |
| format | Article |
| id | doaj-art-233a03c049ec4f1cb91314522328523a |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-233a03c049ec4f1cb91314522328523a2025-02-03T05:15:36ZengTsinghua University PressFood Science and Human Wellness2213-45302023-01-01121161172Fecal metabolomics reveals the positive effect of ethanol extract of propolis on T2DM miceChunmei Wang0Huiting Zhao1Kai Xu2Yali Du3Jinjia Liu4Jinfei Wang5Yusuo Jiang6College of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Life Sciences, Shanxi Agricultural University, Taigu 030801, ChinaJilin Province Institute of Apicultural Science, Jilin 132108, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, China; Corresponding author at: College of Animal Science, Shanxi Agricultural University, Taigu 030801.A large number of studies have shown that propolis has positive effects in the treatment of type 2 diabetes mellitus (T2DM). However, there are have only been a few reports that are based on an ultra-high performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) analysis of the fecal metabolomics of ethanol extract of propolis (EEP) in the treatment of T2DM. The present investigation was designed to screen potential biomarkers of T2DM by the metabonomic method and to explain the possible anti-diabetes mechanism of EEP according to the changes in the biomarkers. The results showed that EEP improved the body weight (BW) of T2DM mice, lowered blood sugar levels, and significantly restored blood biochemical indicators related to T2DM, such as fasting insulin (FINS), homeostasis model assessment of insulin resistance (HOMA-IR), aspartate transaminase (AST), and alanine aminotransferase (ALT). Liver pathology showed that EEP reversed liver damage caused by T2DM. Metabolomics data identified 27 potential biomarkers in fecal samples. EEP effectively regulated the dysfunction in the metabolic pathways of glycerophospholipids, sphingolipids, riboflavins, and sterol lipids caused by T2DM. In summary, our research results revealed positive effects of EEP in the treatment of T2DM and provided potential candidate markers for further research and in the clinical treatment of T2DM.http://www.sciencedirect.com/science/article/pii/S2213453022001410ethanol extract of propolistype 2 diabetes mellitusfecal metabolitesUPLC-Q-TOF-MSmetabolomics |
| spellingShingle | Chunmei Wang Huiting Zhao Kai Xu Yali Du Jinjia Liu Jinfei Wang Yusuo Jiang Fecal metabolomics reveals the positive effect of ethanol extract of propolis on T2DM mice Food Science and Human Wellness ethanol extract of propolis type 2 diabetes mellitus fecal metabolites UPLC-Q-TOF-MS metabolomics |
| title | Fecal metabolomics reveals the positive effect of ethanol extract of propolis on T2DM mice |
| title_full | Fecal metabolomics reveals the positive effect of ethanol extract of propolis on T2DM mice |
| title_fullStr | Fecal metabolomics reveals the positive effect of ethanol extract of propolis on T2DM mice |
| title_full_unstemmed | Fecal metabolomics reveals the positive effect of ethanol extract of propolis on T2DM mice |
| title_short | Fecal metabolomics reveals the positive effect of ethanol extract of propolis on T2DM mice |
| title_sort | fecal metabolomics reveals the positive effect of ethanol extract of propolis on t2dm mice |
| topic | ethanol extract of propolis type 2 diabetes mellitus fecal metabolites UPLC-Q-TOF-MS metabolomics |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001410 |
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