The Role of Cgrp-Receptor Component Protein (Rcp) in Cgrp-Mediated Signal Transduction
The calcitonin gene-related peptide (CGRP)-receptor component protein (RCP) is a 17-kDa intracellular peripheral membrane protein required for signal transduction at CGRP receptors. To determine the role of RCP in CGRP-mediated signal transduction, RCP was depleted from NIH3T3 cells using antisense...
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| Format: | Article |
| Language: | English |
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Wiley
2001-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2001.435 |
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| author | M. A. Prado B. Evans-Bain S. L. Santi I. M. Dickerson |
| author_facet | M. A. Prado B. Evans-Bain S. L. Santi I. M. Dickerson |
| author_sort | M. A. Prado |
| collection | DOAJ |
| description | The calcitonin gene-related peptide (CGRP)-receptor component protein (RCP) is a 17-kDa intracellular peripheral membrane protein required for signal transduction at CGRP receptors. To determine the role of RCP in CGRP-mediated signal transduction, RCP was depleted from NIH3T3 cells using antisense strategy. Loss of RCP protein correlated with loss of cAMP production by CGRP in the antisense cells. In contrast, loss of RCP had no effect on CGRP-mediated binding; therefore RCP is not acting as a chaperone for the CGRP receptor. Instead, RCP is a novel signal transduction molecule that couples the CGRP receptor to the cellular signal transduction machinery. RCP thus represents a prototype for a new class of signal transduction proteins that are required for regulation of G protein-coupled receptors. |
| format | Article |
| id | doaj-art-230ed4fa6daf4a7497f145bebbdaeebc |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2001-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-230ed4fa6daf4a7497f145bebbdaeebc2025-08-20T02:04:34ZengWileyThe Scientific World Journal1537-744X2001-01-011121210.1100/tsw.2001.435The Role of Cgrp-Receptor Component Protein (Rcp) in Cgrp-Mediated Signal TransductionM. A. Prado0B. Evans-Bain1S. L. Santi2I. M. Dickerson3 Department of Physiology and Biophysics, The University of Miami School of Medicine, Miami, FL, USA Department of Physiology and Biophysics, The University of Miami School of Medicine, Miami, FL, USA Department of Physiology and Biophysics, The University of Miami School of Medicine, Miami, FL, USA Department of Physiology and Biophysics, The University of Miami School of Medicine, Miami, FL, USAThe calcitonin gene-related peptide (CGRP)-receptor component protein (RCP) is a 17-kDa intracellular peripheral membrane protein required for signal transduction at CGRP receptors. To determine the role of RCP in CGRP-mediated signal transduction, RCP was depleted from NIH3T3 cells using antisense strategy. Loss of RCP protein correlated with loss of cAMP production by CGRP in the antisense cells. In contrast, loss of RCP had no effect on CGRP-mediated binding; therefore RCP is not acting as a chaperone for the CGRP receptor. Instead, RCP is a novel signal transduction molecule that couples the CGRP receptor to the cellular signal transduction machinery. RCP thus represents a prototype for a new class of signal transduction proteins that are required for regulation of G protein-coupled receptors.http://dx.doi.org/10.1100/tsw.2001.435 |
| spellingShingle | M. A. Prado B. Evans-Bain S. L. Santi I. M. Dickerson The Role of Cgrp-Receptor Component Protein (Rcp) in Cgrp-Mediated Signal Transduction The Scientific World Journal |
| title | The Role of Cgrp-Receptor Component Protein (Rcp) in Cgrp-Mediated Signal Transduction |
| title_full | The Role of Cgrp-Receptor Component Protein (Rcp) in Cgrp-Mediated Signal Transduction |
| title_fullStr | The Role of Cgrp-Receptor Component Protein (Rcp) in Cgrp-Mediated Signal Transduction |
| title_full_unstemmed | The Role of Cgrp-Receptor Component Protein (Rcp) in Cgrp-Mediated Signal Transduction |
| title_short | The Role of Cgrp-Receptor Component Protein (Rcp) in Cgrp-Mediated Signal Transduction |
| title_sort | role of cgrp receptor component protein rcp in cgrp mediated signal transduction |
| url | http://dx.doi.org/10.1100/tsw.2001.435 |
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