Streptomyces griseiviridis sp. nov., a Novel “Modern Actinobacteria” isolated from Malaysia Mangrove Soil

A novel strain, Streptomyces griseiviridis MUM 136JT was recovered from a mangrove forest soil in Malaysia. The Gram-positive bacterium forms strong yellow aerial mycelium and moderate yellow substrate mycelium on ISP 2 agar. A polyphasic approach was used to determine the taxonomy status of strain...

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Main Authors: Jodi Woan Fei Law, Loh Teng-Hern Tan, Vengadesh Letchumanan, Kar-Wai Hong, Hooi-Leng Ser, Bey-Hing Goh, Nurul Syakima Ab Mutalib, Kok-Gan Chan, Learn-Han Lee
Format: Article
Language:English
Published: HH Publisher 2023-01-01
Series:Progress in Microbes and Molecular Biology
Online Access:https://journals.hh-publisher.com/index.php/pmmb/article/view/688
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author Jodi Woan Fei Law
Loh Teng-Hern Tan
Vengadesh Letchumanan
Kar-Wai Hong
Hooi-Leng Ser
Bey-Hing Goh
Nurul Syakima Ab Mutalib
Kok-Gan Chan
Learn-Han Lee
author_facet Jodi Woan Fei Law
Loh Teng-Hern Tan
Vengadesh Letchumanan
Kar-Wai Hong
Hooi-Leng Ser
Bey-Hing Goh
Nurul Syakima Ab Mutalib
Kok-Gan Chan
Learn-Han Lee
author_sort Jodi Woan Fei Law
collection DOAJ
description A novel strain, Streptomyces griseiviridis MUM 136JT was recovered from a mangrove forest soil in Malaysia. The Gram-positive bacterium forms strong yellow aerial mycelium and moderate yellow substrate mycelium on ISP 2 agar. A polyphasic approach was used to determine the taxonomy status of strain MUM 136JT. The strain showed a spectrum of phylogenetic and chemotaxonomic properties consistent with those of the members of the genus Streptomyces. The cell wall peptidoglycan was determined to contain LL-diaminopimelic acid. The predominant menaquinones were identified as MK-9(H8) and MK-9(H6), while the identified polar lipids consisted of lipid, aminolipid, phospholipid, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylinositolmannoside. The cell wall sugars consist of ribose, mannose, and galactose. The predominant cellular fatty acids (>10.0 %) were identified as iso-C16:0 (31.6 %), anteiso-C15:0 (14.8 %), iso-C15:0 (12.0 %), and anteiso-C17:0 (11.1 %). Phylogenetic analysis identified that closely related strains for MUM 136JT are Streptomyces leeuwenhoekii DSM 42122T (98.9 %), Streptomyces erythrogriseus JCM 9650T (98.4 %), Streptomyces griseoincarnatus JCM 4381T (98.5 %). The DNA-DNA relatedness values between MUM 136 JT and closely related type strains ranged from 13.3 ± 1.5 % to 17.4 ± 2.0 %. The name Streptomyces griseiviridis sp. nov. is proposed, and the type strain is MUM 136JT (= NBRC 114249T = MCCC 1K04199T).
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spelling doaj-art-22f69585b39046c8a42c9125fff000702025-02-04T08:39:59ZengHH PublisherProgress in Microbes and Molecular Biology2637-10492023-01-016110.36877/pmmb.a0000270Streptomyces griseiviridis sp. nov., a Novel “Modern Actinobacteria” isolated from Malaysia Mangrove SoilJodi Woan Fei LawLoh Teng-Hern TanVengadesh LetchumananKar-Wai HongHooi-Leng SerBey-Hing GohNurul Syakima Ab MutalibKok-Gan ChanLearn-Han Lee A novel strain, Streptomyces griseiviridis MUM 136JT was recovered from a mangrove forest soil in Malaysia. The Gram-positive bacterium forms strong yellow aerial mycelium and moderate yellow substrate mycelium on ISP 2 agar. A polyphasic approach was used to determine the taxonomy status of strain MUM 136JT. The strain showed a spectrum of phylogenetic and chemotaxonomic properties consistent with those of the members of the genus Streptomyces. The cell wall peptidoglycan was determined to contain LL-diaminopimelic acid. The predominant menaquinones were identified as MK-9(H8) and MK-9(H6), while the identified polar lipids consisted of lipid, aminolipid, phospholipid, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylinositolmannoside. The cell wall sugars consist of ribose, mannose, and galactose. The predominant cellular fatty acids (>10.0 %) were identified as iso-C16:0 (31.6 %), anteiso-C15:0 (14.8 %), iso-C15:0 (12.0 %), and anteiso-C17:0 (11.1 %). Phylogenetic analysis identified that closely related strains for MUM 136JT are Streptomyces leeuwenhoekii DSM 42122T (98.9 %), Streptomyces erythrogriseus JCM 9650T (98.4 %), Streptomyces griseoincarnatus JCM 4381T (98.5 %). The DNA-DNA relatedness values between MUM 136 JT and closely related type strains ranged from 13.3 ± 1.5 % to 17.4 ± 2.0 %. The name Streptomyces griseiviridis sp. nov. is proposed, and the type strain is MUM 136JT (= NBRC 114249T = MCCC 1K04199T). https://journals.hh-publisher.com/index.php/pmmb/article/view/688
spellingShingle Jodi Woan Fei Law
Loh Teng-Hern Tan
Vengadesh Letchumanan
Kar-Wai Hong
Hooi-Leng Ser
Bey-Hing Goh
Nurul Syakima Ab Mutalib
Kok-Gan Chan
Learn-Han Lee
Streptomyces griseiviridis sp. nov., a Novel “Modern Actinobacteria” isolated from Malaysia Mangrove Soil
Progress in Microbes and Molecular Biology
title Streptomyces griseiviridis sp. nov., a Novel “Modern Actinobacteria” isolated from Malaysia Mangrove Soil
title_full Streptomyces griseiviridis sp. nov., a Novel “Modern Actinobacteria” isolated from Malaysia Mangrove Soil
title_fullStr Streptomyces griseiviridis sp. nov., a Novel “Modern Actinobacteria” isolated from Malaysia Mangrove Soil
title_full_unstemmed Streptomyces griseiviridis sp. nov., a Novel “Modern Actinobacteria” isolated from Malaysia Mangrove Soil
title_short Streptomyces griseiviridis sp. nov., a Novel “Modern Actinobacteria” isolated from Malaysia Mangrove Soil
title_sort streptomyces griseiviridis sp nov a novel modern actinobacteria isolated from malaysia mangrove soil
url https://journals.hh-publisher.com/index.php/pmmb/article/view/688
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