Characteristics and Clinical Significance of Gut Microbiota in Patients with Invasive Pulmonary Aspergillosis
Gut microbiota acts on the lungs through the gut-lung axis and play an important role in lung diseases. However, there are no reports on the gut microbiota characteristics in patients with invasive pulmonary aspergillosis (IPA). We aimed to analyze changes in gut microbiota in IPA patients, correlat...
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2025-06-01
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| Series: | Polish Journal of Microbiology |
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| Online Access: | https://doi.org/10.33073/pjm-2025-011 |
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| author | CAO JIAQI HE QIAN ZHANG MING ZHOU RONG FENG CHUNLAI |
| author_facet | CAO JIAQI HE QIAN ZHANG MING ZHOU RONG FENG CHUNLAI |
| author_sort | CAO JIAQI |
| collection | DOAJ |
| description | Gut microbiota acts on the lungs through the gut-lung axis and play an important role in lung diseases. However, there are no reports on the gut microbiota characteristics in patients with invasive pulmonary aspergillosis (IPA). We aimed to analyze changes in gut microbiota in IPA patients, correlate these changes with clinical indicators and disease prognosis, and explore the application value of these characteristic changes in diagnosing IPA. The objective was to provide a theoretical basis for preventing and treating individual immunity. We conducted metagenomic next-generation sequencing of fecal samples from 43 patients with IPA and 31 healthy controls to analyze changes in the gut microbiota of these patients. We also built a random forest model for diagnosing IPA based on the gut microbiota. Compared to healthy controls, IPA patients showed a decrease in gut microbiota diversity and metabolic levels. Changes in the microbiota were characterized by a significant reduction in anti-inflammatory species that produce short-chain fatty acids, such as Faecalibacterium, Blautia, Roseburia, Phocaeicola, and Bacteroides. In contrast, opportunistic pathogens, such as Enterococcus, Corynebacterium, Escherichia, Staphylococcus, Haemophilus, and Finegoldia, were significantly enriched. The classification model based on Clostridium fessum, Blautia wexlerae, Streptococcus pseudopneumoniae, Corynebacterium striatum, and Faecalibacterium prausnitzii showed high value in distinguishing patients with IPA from healthy controls. Patients with IPA exhibit gut microbiota imbalance. The gut microbiota can serve as a biomarker that helps in diagnosing IPA. Our findings support the potential use of gut microbiota as a target for IPA prevention and treatment. |
| format | Article |
| id | doaj-art-22f4aff182cc41bcb4363ed13dcc32ff |
| institution | DOAJ |
| issn | 2544-4646 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj-art-22f4aff182cc41bcb4363ed13dcc32ff2025-08-20T03:22:33ZengSciendoPolish Journal of Microbiology2544-46462025-06-0174213114210.33073/pjm-2025-011Characteristics and Clinical Significance of Gut Microbiota in Patients with Invasive Pulmonary AspergillosisCAO JIAQI0HE QIAN1ZHANG MING2ZHOU RONG3FENG CHUNLAI41Department of Respiratory and Critical Care Medicine, The Third Affiliated Hospital of Soochow University, Jiangsu Changzhou, China1Department of Respiratory and Critical Care Medicine, The Third Affiliated Hospital of Soochow University, Jiangsu Changzhou, China1Department of Respiratory and Critical Care Medicine, The Third Affiliated Hospital of Soochow University, Jiangsu Changzhou, China1Department of Respiratory and Critical Care Medicine, The Third Affiliated Hospital of Soochow University, Jiangsu Changzhou, China1Department of Respiratory and Critical Care Medicine, The Third Affiliated Hospital of Soochow University, Jiangsu Changzhou, ChinaGut microbiota acts on the lungs through the gut-lung axis and play an important role in lung diseases. However, there are no reports on the gut microbiota characteristics in patients with invasive pulmonary aspergillosis (IPA). We aimed to analyze changes in gut microbiota in IPA patients, correlate these changes with clinical indicators and disease prognosis, and explore the application value of these characteristic changes in diagnosing IPA. The objective was to provide a theoretical basis for preventing and treating individual immunity. We conducted metagenomic next-generation sequencing of fecal samples from 43 patients with IPA and 31 healthy controls to analyze changes in the gut microbiota of these patients. We also built a random forest model for diagnosing IPA based on the gut microbiota. Compared to healthy controls, IPA patients showed a decrease in gut microbiota diversity and metabolic levels. Changes in the microbiota were characterized by a significant reduction in anti-inflammatory species that produce short-chain fatty acids, such as Faecalibacterium, Blautia, Roseburia, Phocaeicola, and Bacteroides. In contrast, opportunistic pathogens, such as Enterococcus, Corynebacterium, Escherichia, Staphylococcus, Haemophilus, and Finegoldia, were significantly enriched. The classification model based on Clostridium fessum, Blautia wexlerae, Streptococcus pseudopneumoniae, Corynebacterium striatum, and Faecalibacterium prausnitzii showed high value in distinguishing patients with IPA from healthy controls. Patients with IPA exhibit gut microbiota imbalance. The gut microbiota can serve as a biomarker that helps in diagnosing IPA. Our findings support the potential use of gut microbiota as a target for IPA prevention and treatment.https://doi.org/10.33073/pjm-2025-011invasive pulmonary aspergillosisgut microbiotamicrobial diversitymetagenomic next-generation sequencingrandom forest |
| spellingShingle | CAO JIAQI HE QIAN ZHANG MING ZHOU RONG FENG CHUNLAI Characteristics and Clinical Significance of Gut Microbiota in Patients with Invasive Pulmonary Aspergillosis Polish Journal of Microbiology invasive pulmonary aspergillosis gut microbiota microbial diversity metagenomic next-generation sequencing random forest |
| title | Characteristics and Clinical Significance of Gut Microbiota in Patients with Invasive Pulmonary Aspergillosis |
| title_full | Characteristics and Clinical Significance of Gut Microbiota in Patients with Invasive Pulmonary Aspergillosis |
| title_fullStr | Characteristics and Clinical Significance of Gut Microbiota in Patients with Invasive Pulmonary Aspergillosis |
| title_full_unstemmed | Characteristics and Clinical Significance of Gut Microbiota in Patients with Invasive Pulmonary Aspergillosis |
| title_short | Characteristics and Clinical Significance of Gut Microbiota in Patients with Invasive Pulmonary Aspergillosis |
| title_sort | characteristics and clinical significance of gut microbiota in patients with invasive pulmonary aspergillosis |
| topic | invasive pulmonary aspergillosis gut microbiota microbial diversity metagenomic next-generation sequencing random forest |
| url | https://doi.org/10.33073/pjm-2025-011 |
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